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- W1948692008 abstract "Abstract We describe the determination of ethosuximide and desmethylmethsuximide, simultaneously or separately, in 50 to 100 microliter of plasma or serum. Derivatives of ethosuximide and desmethylmethsuximide formed by reaction with pentafluorobenzoyl chloride are extremely sensitive to the electron-capture detector of a gas-liquid chromatograph. The sample, with added internal standard and ammonium sulfate as a pH-adjusting and salting-out agent, is extracted with ethyl acetate/benzene (20/80 by vol). The extract is evaporated and the derivatives are formed. Analytical recoveries of ethosuximide and desmethylmethsuximide exceed 99%, and the relative standard deviation (CV) between analyses is usually less than 4.0%. alpha-Methyl-alpha-propylsuccinimide is used as the internal standard for ethosuximide, 2-phenylsuccinimide as the internal standard for desmethylmethsuximide." @default.
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- W1948692008 date "1979-02-01" @default.
- W1948692008 modified "2023-10-14" @default.
- W1948692008 title "Electron-capture gas-liquid chromatographic determination of ethosuximide and desmethylmethsuximide in plasma or serum." @default.
- W1948692008 doi "https://doi.org/10.1093/clinchem/25.2.252" @default.
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