FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1948806874> ?p ?o ?g. }

• W1948806874 endingPage "330" @default.
• W1948806874 startingPage "320" @default.
• W1948806874 abstract "A major tripartite multidrug efflux pump of Escherichia coli, AcrAB-TolC, confers resistance to a wide variety of compounds. The drug molecule is captured by AcrB probably from the periplasm or the periplasm/inner membrane interface, and is passed through AcrB and then TolC to the medium. Currently, there exist numerous crystallographic and mutation data concerning the regions of AcrB and its homologues that may interact with substrates. Starting with these data, we devised fluorescence assays in whole cells to determine the entire substrate path through AcrB. We tested 48 residues in AcrB along the predicted substrate path and 25 gave positive results, based on the covalent labelling of cysteine residues by a lipophilic dye-maleimide and the blocking of Nile red efflux by covalent labelling with bulky maleimide reagents. These residues are all located in the periplasmic domain, in regions we designate as the lower part of the large external cleft, the cleft itself, the crystallographically defined binding pocket, and the gate between the pocket and the funnel. Our observations suggest that the substrate is captured in the lower cleft region of AcrB, then transported through the binding pocket, the gate and finally to the AcrB funnel that connects AcrB to TolC." @default.
• W1948806874 created "2016-06-24" @default.
• W1948806874 creator A5052582801 @default.
• W1948806874 creator A5086380170 @default.
• W1948806874 date "2010-08-20" @default.
• W1948806874 modified "2023-10-15" @default.
• W1948806874 title "Substrate path in the AcrB multidrug efflux pump of Escherichia coli" @default.
• W1948806874 cites W1573080626 @default.
• W1948806874 cites W1892148740 @default.
• W1948806874 cites W1908114653 @default.
• W1948806874 cites W1924074075 @default.
• W1948806874 cites W1934127833 @default.
• W1948806874 cites W1942332768 @default.
• W1948806874 cites W1977645333 @default.
• W1948806874 cites W1985400607 @default.
• W1948806874 cites W1987009053 @default.
• W1948806874 cites W1999099977 @default.
• W1948806874 cites W2004522996 @default.
• W1948806874 cites W2010438788 @default.
• W1948806874 cites W2018396099 @default.
• W1948806874 cites W2038684541 @default.
• W1948806874 cites W2047908973 @default.
• W1948806874 cites W2051405707 @default.
• W1948806874 cites W2061528271 @default.
• W1948806874 cites W2061804805 @default.
• W1948806874 cites W2070196678 @default.
• W1948806874 cites W2077534797 @default.
• W1948806874 cites W2077815188 @default.
• W1948806874 cites W2084778413 @default.
• W1948806874 cites W2096172849 @default.
• W1948806874 cites W2102743211 @default.
• W1948806874 cites W2109731964 @default.
• W1948806874 cites W2111464826 @default.
• W1948806874 cites W2112903691 @default.
• W1948806874 cites W2118528768 @default.
• W1948806874 cites W2129762213 @default.
• W1948806874 cites W2131080603 @default.
• W1948806874 cites W2132067013 @default.
• W1948806874 cites W2138354421 @default.
• W1948806874 cites W2142318169 @default.
• W1948806874 cites W2143095073 @default.
• W1948806874 cites W2149485676 @default.
• W1948806874 cites W2150015383 @default.
• W1948806874 cites W2150093310 @default.
• W1948806874 cites W2152087737 @default.
• W1948806874 cites W2158530003 @default.
• W1948806874 doi "https://doi.org/10.1111/j.1365-2958.2010.07330.x" @default.
• W1948806874 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3008161" @default.
• W1948806874 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20804453" @default.
• W1948806874 hasPublicationYear "2010" @default.
• W1948806874 type Work @default.
• W1948806874 sameAs 1948806874 @default.
• W1948806874 citedByCount "81" @default.
• W1948806874 countsByYear W19488068742012 @default.
• W1948806874 countsByYear W19488068742013 @default.
• W1948806874 countsByYear W19488068742014 @default.
• W1948806874 countsByYear W19488068742015 @default.
• W1948806874 countsByYear W19488068742016 @default.
• W1948806874 countsByYear W19488068742017 @default.
• W1948806874 countsByYear W19488068742018 @default.
• W1948806874 countsByYear W19488068742019 @default.
• W1948806874 countsByYear W19488068742020 @default.
• W1948806874 countsByYear W19488068742021 @default.
• W1948806874 countsByYear W19488068742022 @default.
• W1948806874 countsByYear W19488068742023 @default.
• W1948806874 crossrefType "journal-article" @default.
• W1948806874 hasAuthorship W1948806874A5052582801 @default.
• W1948806874 hasAuthorship W1948806874A5086380170 @default.
• W1948806874 hasBestOaLocation W19488068741 @default.
• W1948806874 hasConcept C104317684 @default.
• W1948806874 hasConcept C115335371 @default.
• W1948806874 hasConcept C12554922 @default.
• W1948806874 hasConcept C144647389 @default.
• W1948806874 hasConcept C149011108 @default.
• W1948806874 hasConcept C181199279 @default.
• W1948806874 hasConcept C200082930 @default.
• W1948806874 hasConcept C201663137 @default.
• W1948806874 hasConcept C2779201268 @default.
• W1948806874 hasConcept C2910769707 @default.
• W1948806874 hasConcept C41625074 @default.
• W1948806874 hasConcept C44312359 @default.
• W1948806874 hasConcept C547475151 @default.
• W1948806874 hasConcept C55493867 @default.
• W1948806874 hasConcept C65871279 @default.
• W1948806874 hasConcept C86803240 @default.
• W1948806874 hasConceptScore W1948806874C104317684 @default.
• W1948806874 hasConceptScore W1948806874C115335371 @default.
• W1948806874 hasConceptScore W1948806874C12554922 @default.
• W1948806874 hasConceptScore W1948806874C144647389 @default.
• W1948806874 hasConceptScore W1948806874C149011108 @default.
• W1948806874 hasConceptScore W1948806874C181199279 @default.
• W1948806874 hasConceptScore W1948806874C200082930 @default.
• W1948806874 hasConceptScore W1948806874C201663137 @default.
• W1948806874 hasConceptScore W1948806874C2779201268 @default.
• W1948806874 hasConceptScore W1948806874C2910769707 @default.
• W1948806874 hasConceptScore W1948806874C41625074 @default.
• W1948806874 hasConceptScore W1948806874C44312359 @default.
• W1948806874 hasConceptScore W1948806874C547475151 @default.

• next