FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1949429952> ?p ?o ?g. }

• W1949429952 endingPage "3142" @default.
• W1949429952 startingPage "3133" @default.
• W1949429952 abstract "The recombinant polymerase protein L of vesicular stomatitis virus (VSV) expressed in COS cells is able to transcribe and replicate the viral genome, resulting in complementation of temperature-sensitive polymerase mutants of VSV at the restrictive temperature (M. Schubert, G. G. Harmison, C. D. Richardson, and E. Meier, Proc. Natl. Acad. Sci. USA 82:7984-7988, 1985). Here we report that the efficiency of complementation is dependent on the level of L protein expression. Unexpectedly, only cells expressing low levels of recombinant L protein efficiently complemented tsL gene mutants, whereas cells with high levels of L protein did not. In fact, in all cells with high levels of L protein expression, which at 40 h posttransfection represented almost the total number of transfected cells, viral replication not only of the temperature-sensitive mutant but also of wild-type VSV was excluded. The inhibition of VSV appeared to occur at an early stage of the infectious cycle, and wild-type virus of the same serotype (Indiana) as the recombinant L protein as well as wild-type virus of a different serotype (New Jersey) was affected. Measles virus, on the other hand, was not arrested in cells with high levels of recombinant L protein, demonstrating that these cells were still capable of supporting a viral infection. The expression of high levels of only the amino-terminal half of the L protein from a recombinant mutant L gene that contains a small out-of-frame deletion in the middle of the L gene did not inhibit a VSV infection. Since the level of amplification for both L- and truncated L-encoding vectors is similar, we conclude that the arrest of VSV was caused by high levels of functional full-length L protein itself and not by high levels of vector-encoded L mRNA or other vector products or by side effects of vector amplification. These data strongly support the idea that the highly conserved gene order of nonsegmented negative-strand viruses and the sequential and attenuated mode of transcription are important regulatory elements which balance the intracellular concentration of viral proteins. They both assure that the L gene is the last and the least frequently transcribed gene, giving rise to low levels of L protein necessary for efficient replication." @default.
• W1949429952 created "2016-06-24" @default.
• W1949429952 creator A5027413431 @default.
• W1949429952 creator A5058547446 @default.
• W1949429952 creator A5078618860 @default.
• W1949429952 date "1987-10-01" @default.
• W1949429952 modified "2023-09-27" @default.
• W1949429952 title "Homotypic and heterotypic exclusion of vesicular stomatitis virus replication by high levels of recombinant polymerase protein L" @default.
• W1949429952 cites W1524112058 @default.
• W1949429952 cites W1541571765 @default.
• W1949429952 cites W1550101617 @default.
• W1949429952 cites W1556589224 @default.
• W1949429952 cites W1603264084 @default.
• W1949429952 cites W1640479649 @default.
• W1949429952 cites W1665121191 @default.
• W1949429952 cites W1812914533 @default.
• W1949429952 cites W1830441222 @default.
• W1949429952 cites W1967446284 @default.
• W1949429952 cites W1969542352 @default.
• W1949429952 cites W2008268465 @default.
• W1949429952 cites W2033341519 @default.
• W1949429952 cites W2041662176 @default.
• W1949429952 cites W2042741115 @default.
• W1949429952 cites W2063025555 @default.
• W1949429952 cites W2063929683 @default.
• W1949429952 cites W2088167128 @default.
• W1949429952 cites W2109030204 @default.
• W1949429952 cites W2113208052 @default.
• W1949429952 cites W2115083799 @default.
• W1949429952 cites W2118541996 @default.
• W1949429952 cites W2131706430 @default.
• W1949429952 cites W2147703582 @default.
• W1949429952 cites W2166239756 @default.
• W1949429952 doi "https://doi.org/10.1128/jvi.61.10.3133-3142.1987" @default.
• W1949429952 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/255890" @default.
• W1949429952 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3041035" @default.
• W1949429952 hasPublicationYear "1987" @default.
• W1949429952 type Work @default.
• W1949429952 sameAs 1949429952 @default.
• W1949429952 citedByCount "32" @default.
• W1949429952 crossrefType "journal-article" @default.
• W1949429952 hasAuthorship W1949429952A5027413431 @default.
• W1949429952 hasAuthorship W1949429952A5058547446 @default.
• W1949429952 hasAuthorship W1949429952A5078618860 @default.
• W1949429952 hasBestOaLocation W19494299521 @default.
• W1949429952 hasConcept C104317684 @default.
• W1949429952 hasConcept C140704245 @default.
• W1949429952 hasConcept C143065580 @default.
• W1949429952 hasConcept C153911025 @default.
• W1949429952 hasConcept C159047783 @default.
• W1949429952 hasConcept C188082640 @default.
• W1949429952 hasConcept C2522874641 @default.
• W1949429952 hasConcept C2777974785 @default.
• W1949429952 hasConcept C2779057330 @default.
• W1949429952 hasConcept C39060996 @default.
• W1949429952 hasConcept C40767141 @default.
• W1949429952 hasConcept C54009773 @default.
• W1949429952 hasConcept C54355233 @default.
• W1949429952 hasConcept C86803240 @default.
• W1949429952 hasConceptScore W1949429952C104317684 @default.
• W1949429952 hasConceptScore W1949429952C140704245 @default.
• W1949429952 hasConceptScore W1949429952C143065580 @default.
• W1949429952 hasConceptScore W1949429952C153911025 @default.
• W1949429952 hasConceptScore W1949429952C159047783 @default.
• W1949429952 hasConceptScore W1949429952C188082640 @default.
• W1949429952 hasConceptScore W1949429952C2522874641 @default.
• W1949429952 hasConceptScore W1949429952C2777974785 @default.
• W1949429952 hasConceptScore W1949429952C2779057330 @default.
• W1949429952 hasConceptScore W1949429952C39060996 @default.
• W1949429952 hasConceptScore W1949429952C40767141 @default.
• W1949429952 hasConceptScore W1949429952C54009773 @default.
• W1949429952 hasConceptScore W1949429952C54355233 @default.
• W1949429952 hasConceptScore W1949429952C86803240 @default.
• W1949429952 hasIssue "10" @default.
• W1949429952 hasLocation W19494299521 @default.
• W1949429952 hasLocation W19494299522 @default.
• W1949429952 hasLocation W19494299523 @default.
• W1949429952 hasLocation W19494299524 @default.
• W1949429952 hasOpenAccess W1949429952 @default.
• W1949429952 hasPrimaryLocation W19494299521 @default.
• W1949429952 hasRelatedWork W1554885052 @default.
• W1949429952 hasRelatedWork W1949429952 @default.
• W1949429952 hasRelatedWork W1989357722 @default.
• W1949429952 hasRelatedWork W2028992925 @default.
• W1949429952 hasRelatedWork W2035774172 @default.
• W1949429952 hasRelatedWork W2048683885 @default.
• W1949429952 hasRelatedWork W2124595898 @default.
• W1949429952 hasRelatedWork W2151611155 @default.
• W1949429952 hasRelatedWork W2312143864 @default.
• W1949429952 hasRelatedWork W4319756017 @default.
• W1949429952 hasVolume "61" @default.
• W1949429952 isParatext "false" @default.
• W1949429952 isRetracted "false" @default.
• W1949429952 magId "1949429952" @default.
• W1949429952 workType "article" @default.