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- W194955255 abstract "This chapter provides an overview of the properties of the vaccinia capping enzyme, detailed protocols for enzyme assay and purification, and examples of how the purified enzyme may be of use to biochemists and molecular biologists. As the vaccinia capping enzyme exists as a complex of RNA triphosphatase, RNA guanylyltransferase, and RNA (guanine-7)-methyltransferase activities, any one of the three functions may be used to monitor enzyme activity during purification. Polyadenylic acid with a 5′-triphosphate terminus is generally used as the cap acceptor for assay purposes, and it may be synthesized by Escherichia coli RNA polymerase using the following protocol. Electrophoresis must be carried out until the dye front is well out of the bottom of the gel. The samples may be precipitated with TCA after denaturation with SDS and the acid-insoluble radioactivity determined by collection on glass fiber filters. Vaccinia is stable at ambient temperature even when dried, and parenteral inoculation and droplet or aerosol exposure of mucous membranes or broken skin with infectious virus are the primary hazards to laboratory personnel." @default.
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- W194955255 date "1990-01-01" @default.
- W194955255 modified "2023-09-23" @default.
- W194955255 title "Purification and use of vaccinia virus messenger RNA capping enzyme" @default.
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- W194955255 doi "https://doi.org/10.1016/0076-6879(90)81119-f" @default.
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