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• W1950038354 endingPage "850" @default.
• W1950038354 startingPage "837" @default.
• W1950038354 abstract "Abstract Insertions of the yeast element Ty3 resulting from induced retrotransposition were characterized in order to identify the genomic targets of transposition. The DNA sequences of the junctions between Ty3 and flanking DNA were determined for two insertions of an unmarked element. Each insertion was at position -17 from the 5' end of a tRNA-coding sequence. Ninety-one independent insertions of a marked Ty3 element were studied by Southern blot analysis. Pairs of independent insertions into seven genomic loci accounted for 14 of these insertions. The DNA sequence flanking the insertion site was determined for at least one member of each pair of integrated elements. In each case, insertion was at position -16 or -17 relative to the 5' end of one of seven different tRNA genes. This proportion of genomic loci used twice for Ty3 integration is consistent with that predicted by a Poisson distribution for a number of genomic targets roughly equivalent to the estimated number of yeast tRNA genes. In addition, insertions upstream of the same tRNA gene in one case were at different positions, but in all cases were in the same orientation. Thus, genomic insertions of Ty3 in a particular orientation are apparently specified by the target, while the actual position of the insertion relative to the tRNA-coding sequence can vary slightly." @default.
• W1950038354 created "2016-06-24" @default.
• W1950038354 creator A5029367275 @default.
• W1950038354 creator A5075351356 @default.
• W1950038354 date "1990-12-01" @default.
• W1950038354 modified "2023-10-17" @default.
• W1950038354 title "Transfer RNA genes are genomic targets for de Novo transposition of the yeast retrotransposon Ty3." @default.
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• W1950038354 doi "https://doi.org/10.1093/genetics/126.4.837" @default.
• W1950038354 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1204282" @default.
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