Matches in SemOpenAlex for { <https://semopenalex.org/work/W1950481374> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W1950481374 endingPage "3784" @default.
- W1950481374 startingPage "3775" @default.
- W1950481374 abstract "Abstract It is well established that mast cell proliferation and maturation are regulated by two principle cytokines, IL-3 and the c-kit ligand stem cell factor (SCF). Little is known, however, how these two processes are negatively regulated and thus, how mast cell number is controlled in normal or pathologic processes. In this study we hypothesized that IL-3-dependent mast cells would undergo programmed cell death (apoptosis) on removal of IL-3 as was shown with other growth factor-dependent hemopoietic cells. Apoptotic changes were analyzed using light microscopy, fluorescent staining with acridine orange, flow cytometric analysis, and DNA electrophoresis. We could demonstrate that elimination of IL-3 from either primary bone marrow-derived cultured mast cell cultures (BMCMC) or from the growth factor-dependent mast cell line MCP5 resulted in the characteristic changes of apoptosis including condensed chromatin, fragmented nuclei, cellular vacuolization, typical pattern of propidium iodide or Hoechst 33342 uptake by flow cytometry, and the characteristic 200 bp ladder pattern of DNA cleavage. These events were prevented by SCF, an action that was in part mediated by tyrosine kinases, in that the tyrosine kinase inhibitor herbimycin inhibited the action of SCF in preventing apoptosis in IL-3-deprived cells. By using anti c-kit mAb and IL-3-dependent BMCMC obtained from W/Wv mice homozygous for mutation at the w locus that encodes the c-kit receptor, we could also show that SCF exerted its effect via c-kit. Neither dexamethasone nor cyclosporin A inhibited the rescue effect of SCF, suggesting that rescue was mediated by SCF and not through the induction of other cytokines. Thus, IL-3-dependent mast cells undergo apoptosis on removal of IL-3, an event that is prevented by the addition of SCF through its ligand c-kit, thus demonstrating how these principle mast cell growth factors may act in concert to regulate mast cell number under physiologic conditions." @default.
- W1950481374 created "2016-06-24" @default.
- W1950481374 creator A5004937142 @default.
- W1950481374 creator A5036928204 @default.
- W1950481374 creator A5038329460 @default.
- W1950481374 date "1993-10-01" @default.
- W1950481374 modified "2023-10-16" @default.
- W1950481374 title "IL-3-dependent murine mast cells undergo apoptosis on removal of IL-3. Prevention of apoptosis by c-kit ligand." @default.
- W1950481374 doi "https://doi.org/10.4049/jimmunol.151.7.3775" @default.
- W1950481374 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7690814" @default.
- W1950481374 hasPublicationYear "1993" @default.
- W1950481374 type Work @default.
- W1950481374 sameAs 1950481374 @default.
- W1950481374 citedByCount "146" @default.
- W1950481374 countsByYear W19504813742012 @default.
- W1950481374 countsByYear W19504813742013 @default.
- W1950481374 countsByYear W19504813742014 @default.
- W1950481374 countsByYear W19504813742018 @default.
- W1950481374 countsByYear W19504813742019 @default.
- W1950481374 countsByYear W19504813742021 @default.
- W1950481374 countsByYear W19504813742022 @default.
- W1950481374 countsByYear W19504813742023 @default.
- W1950481374 crossrefType "journal-article" @default.
- W1950481374 hasAuthorship W1950481374A5004937142 @default.
- W1950481374 hasAuthorship W1950481374A5036928204 @default.
- W1950481374 hasAuthorship W1950481374A5038329460 @default.
- W1950481374 hasBestOaLocation W19504813741 @default.
- W1950481374 hasConcept C109159458 @default.
- W1950481374 hasConcept C153911025 @default.
- W1950481374 hasConcept C185592680 @default.
- W1950481374 hasConcept C190283241 @default.
- W1950481374 hasConcept C2022786 @default.
- W1950481374 hasConcept C203014093 @default.
- W1950481374 hasConcept C2775934118 @default.
- W1950481374 hasConcept C2779726688 @default.
- W1950481374 hasConcept C28328180 @default.
- W1950481374 hasConcept C31573885 @default.
- W1950481374 hasConcept C43759708 @default.
- W1950481374 hasConcept C502942594 @default.
- W1950481374 hasConcept C55493867 @default.
- W1950481374 hasConcept C86803240 @default.
- W1950481374 hasConcept C95444343 @default.
- W1950481374 hasConceptScore W1950481374C109159458 @default.
- W1950481374 hasConceptScore W1950481374C153911025 @default.
- W1950481374 hasConceptScore W1950481374C185592680 @default.
- W1950481374 hasConceptScore W1950481374C190283241 @default.
- W1950481374 hasConceptScore W1950481374C2022786 @default.
- W1950481374 hasConceptScore W1950481374C203014093 @default.
- W1950481374 hasConceptScore W1950481374C2775934118 @default.
- W1950481374 hasConceptScore W1950481374C2779726688 @default.
- W1950481374 hasConceptScore W1950481374C28328180 @default.
- W1950481374 hasConceptScore W1950481374C31573885 @default.
- W1950481374 hasConceptScore W1950481374C43759708 @default.
- W1950481374 hasConceptScore W1950481374C502942594 @default.
- W1950481374 hasConceptScore W1950481374C55493867 @default.
- W1950481374 hasConceptScore W1950481374C86803240 @default.
- W1950481374 hasConceptScore W1950481374C95444343 @default.
- W1950481374 hasIssue "7" @default.
- W1950481374 hasLocation W19504813741 @default.
- W1950481374 hasLocation W19504813742 @default.
- W1950481374 hasOpenAccess W1950481374 @default.
- W1950481374 hasPrimaryLocation W19504813741 @default.
- W1950481374 hasRelatedWork W1968505653 @default.
- W1950481374 hasRelatedWork W19916217 @default.
- W1950481374 hasRelatedWork W2025297671 @default.
- W1950481374 hasRelatedWork W2056744132 @default.
- W1950481374 hasRelatedWork W2094270522 @default.
- W1950481374 hasRelatedWork W2169503878 @default.
- W1950481374 hasRelatedWork W2370467556 @default.
- W1950481374 hasRelatedWork W2618206755 @default.
- W1950481374 hasRelatedWork W4220681557 @default.
- W1950481374 hasRelatedWork W4238227746 @default.
- W1950481374 hasVolume "151" @default.
- W1950481374 isParatext "false" @default.
- W1950481374 isRetracted "false" @default.
- W1950481374 magId "1950481374" @default.
- W1950481374 workType "article" @default.