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• W1950488331 endingPage "10382" @default.
• W1950488331 startingPage "10371" @default.
• W1950488331 abstract "Previously, no retroviral Gag protein has been highly purified in milligram quantities and in a biologically relevant and active form. We have purified Rous sarcoma virus (RSV) Gag protein and in parallel several truncation mutants of Gag and have studied their biophysical properties and membrane interactions in vitro. RSV Gag is unusual in that it is not naturally myristoylated. From its ability to assemble into virus-like particles in vitro, we infer that RSV Gag is biologically active. By size exclusion chromatography and small-angle X-ray scattering, Gag in solution appears extended and flexible, in contrast to previous reports on unmyristoylated HIV-1 Gag, which is compact. However, by neutron reflectometry measurements of RSV Gag bound to a supported bilayer, the protein appears to adopt a more compact, folded-over conformation. At physiological ionic strength, purified Gag binds strongly to liposomes containing acidic lipids. This interaction is stimulated by physiological levels of phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2] and by cholesterol. However, unlike HIV-1 Gag, RSV Gag shows no sensitivity to acyl chain saturation. In contrast with full-length RSV Gag, the purified MA domain of Gag binds to liposomes only weakly. Similarly, both an N-terminally truncated version of Gag that is missing the MA domain and a C-terminally truncated version that is missing the NC domain bind only weakly. These results imply that NC contributes to membrane interaction in vitro, either by directly contacting acidic lipids or by promoting Gag multimerization.Retroviruses like HIV assemble at and bud from the plasma membrane of cells. Assembly requires the interaction between thousands of Gag molecules to form a lattice. Previous work indicated that lattice formation at the plasma membrane is influenced by the conformation of monomeric HIV. We have extended this work to the more tractable RSV Gag. Our results show that RSV Gag is highly flexible and can adopt a folded-over conformation on a lipid bilayer, implicating both the N and C termini in membrane binding. In addition, binding of Gag to membranes is diminished when either terminal domain is truncated. RSV Gag membrane association is significantly less sensitive than HIV Gag membrane association to lipid acyl chain saturation. These findings shed light on Gag assembly and membrane binding, critical steps in the viral life cycle and an untapped target for antiretroviral drugs." @default.
• W1950488331 created "2016-06-24" @default.
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• W1950488331 date "2015-10-15" @default.
• W1950488331 modified "2023-10-01" @default.
• W1950488331 title "Hydrodynamic and Membrane Binding Properties of Purified Rous Sarcoma Virus Gag Protein" @default.
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• W1950488331 cites W1894623846 @default.
• W1950488331 cites W1933776452 @default.
• W1950488331 cites W1967986789 @default.
• W1950488331 cites W1972717662 @default.
• W1950488331 cites W1973475612 @default.
• W1950488331 cites W1973807130 @default.
• W1950488331 cites W1977038370 @default.
• W1950488331 cites W1980268459 @default.
• W1950488331 cites W1982126789 @default.
• W1950488331 cites W1985219150 @default.
• W1950488331 cites W1986142812 @default.
• W1950488331 cites W1989054182 @default.
• W1950488331 cites W1991531197 @default.
• W1950488331 cites W1991879937 @default.
• W1950488331 cites W1994899765 @default.
• W1950488331 cites W1995334678 @default.
• W1950488331 cites W1995633927 @default.
• W1950488331 cites W1997860232 @default.
• W1950488331 cites W1997940831 @default.
• W1950488331 cites W2000569926 @default.
• W1950488331 cites W2007311551 @default.
• W1950488331 cites W2007527881 @default.
• W1950488331 cites W2008304861 @default.
• W1950488331 cites W2017522157 @default.
• W1950488331 cites W2028059774 @default.
• W1950488331 cites W2041728208 @default.
• W1950488331 cites W2052686426 @default.
• W1950488331 cites W2055782613 @default.
• W1950488331 cites W2057291641 @default.
• W1950488331 cites W2057712924 @default.
• W1950488331 cites W2057723413 @default.
• W1950488331 cites W2057932255 @default.
• W1950488331 cites W2059957397 @default.
• W1950488331 cites W2066158357 @default.
• W1950488331 cites W2067054157 @default.
• W1950488331 cites W2068446921 @default.
• W1950488331 cites W2072435127 @default.
• W1950488331 cites W2074560169 @default.
• W1950488331 cites W2077697876 @default.
• W1950488331 cites W2087978946 @default.
• W1950488331 cites W2092363046 @default.
• W1950488331 cites W2096621401 @default.
• W1950488331 cites W2099416002 @default.
• W1950488331 cites W2105093369 @default.
• W1950488331 cites W2107120241 @default.
• W1950488331 cites W2113227718 @default.
• W1950488331 cites W2115644744 @default.
• W1950488331 cites W2117867057 @default.
• W1950488331 cites W2120106543 @default.
• W1950488331 cites W2125872855 @default.
• W1950488331 cites W2134283668 @default.
• W1950488331 cites W2134444484 @default.
• W1950488331 cites W2134798467 @default.
• W1950488331 cites W2137401529 @default.
• W1950488331 cites W2138720208 @default.
• W1950488331 cites W2139457276 @default.
• W1950488331 cites W2142654956 @default.
• W1950488331 cites W2147792469 @default.
• W1950488331 cites W2148956213 @default.
• W1950488331 cites W2152534499 @default.
• W1950488331 cites W2153337961 @default.
• W1950488331 cites W2156651686 @default.
• W1950488331 cites W2157103983 @default.
• W1950488331 cites W2159148283 @default.
• W1950488331 cites W2167615773 @default.
• W1950488331 cites W2167680425 @default.
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• W1950488331 doi "https://doi.org/10.1128/jvi.01628-15" @default.
• W1950488331 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4580166" @default.
• W1950488331 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26246573" @default.
• W1950488331 hasPublicationYear "2015" @default.
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