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- W1950634725 abstract "Exposure of ribose-grown Hydrogenomonas facilis to 14 CO 2 for 6 to 12 sec during ribose oxidation resulted in labeling of a number of compounds, three of which were glutamate, phosphoglycerate, and pyruvate. Phosphoglycerate and pyruvate were labeled almost exclusively in C 1 , suggesting operation of the reductive pentose phosphate cycle. Glutamate was labeled initially to the extent of 90% in C 1 and 10% in C 5 , and this was followed by a concentration of radioisotope in C 5 . All of the enzymes of the tricarboxylic acid cycle were detectable in ribose-grown cells, and, in general, specific activities were similar to those found in yeast extract-grown cells. Reduced nicotinamide adenine dinucleotide oxidase, aconitase, and the dehydrogenases for pyruvate, α-ketoglutarate, and succinate appeared to be of particulate origin. In addition to enzymes of the tricarboxylic acid cycle, an acetyl coenzyme A-stimulated phosphoenolpyruvate carboxylase was found, as was isocitrate lyase. Possible participation of these catalysts in glutamate synthesis is discussed." @default.
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- W1950634725 date "1967-03-01" @default.
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- W1950634725 title "14 CO 2 Fixation, Glutamate Labeling, and the Krebs Cycle in Ribose-grown Hydrogenomonas facilis" @default.
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- W1950634725 doi "https://doi.org/10.1128/jb.93.3.879-885.1967" @default.
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