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• W1950635443 endingPage "8259" @default.
• W1950635443 startingPage "8255" @default.
• W1950635443 abstract "Chimeric cDNAs encoding a sarcoplasmic/endoplasmic reticulum Ca-ATPase (SERCA1) and regions of the Na,K-ATPase alpha-subunit were constructed to seek the minimal region of the alpha-subunit sufficient for assembly with the Na,K-ATPase beta-subunit. cDNAs encoding a chimera and the chicken beta-subunit were coexpressed in mammalian cells and assembly was assayed by immune precipitation of the chimeric subunit with a monoclonal antibody to the chicken beta-subunit. A chimera containing 26 amino acyl residues of the Na,K-ATPase alpha 1-subunit (NDVEDSYGQQWTFEQRKIVEFTCHTA) (Asn894 to Ala919) that replaced the corresponding avian SERCA1 Ca-ATPase amino acyl residues (Thr871 to Thr898) was able to assemble with the chicken beta-subunit. This alpha-subunit region is predicted to be extracellular, located between membrane-spanning domains 7 and 8 (H7-H8). Chimeras that assembled with full-length beta-subunit also assembled with a beta-subunit chimera that retained only the ectodomain of the chicken beta 1-subunit. These results suggest that the Na,K-ATPase alpha-subunit has the same topology in the membrane as the sarcoplasmic reticulum Ca-ATPase, probably with 10 membrane-spanning domains, and that the aminoacyl residues between membrane domains H7 and H8 are involved in assembly with the beta-subunit in the extracellular/lumenal space." @default.
• W1950635443 created "2016-06-24" @default.
• W1950635443 creator A5034416633 @default.
• W1950635443 creator A5046748617 @default.
• W1950635443 creator A5058105195 @default.
• W1950635443 creator A5082722882 @default.
• W1950635443 date "1994-03-01" @default.
• W1950635443 modified "2023-10-13" @default.
• W1950635443 title "26 amino acids of an extracellular domain of the Na,K-ATPase alpha-subunit are sufficient for assembly with the Na,K-ATPase beta-subunit." @default.
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• W1950635443 doi "https://doi.org/10.1016/s0021-9258(17)37187-9" @default.
• W1950635443 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7907590" @default.
• W1950635443 hasPublicationYear "1994" @default.
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