FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1950929708> ?p ?o ?g. }

• W1950929708 endingPage "142" @default.
• W1950929708 startingPage "131" @default.
• W1950929708 abstract "Förster resonance energy transfer (FRET) microscopy at the single molecule level has the potential to yield information on intra and intermolecular distances within the 2–10 nm range of molecules or molecular complexes that undergo frequent conformation changes. A pre-requirement for obtaining accurate distance information is to determine quantitative instrument independent FRET efficiency values. Here, we applied and evaluated a procedure to determine quantitative FRET efficiencies directly from individual fluorescence time traces of surface immobilized DNA molecules without the need for external calibrants. To probe the robustness of the approach over a wide range of FRET efficiencies we used a set of doubly labelled double stranded DNA samples, where the acceptor position was varied systematically. Interestingly, we found that fluorescence contributions arising from direct acceptor excitation following donor excitation are intrinsically taken into account in these conditions as other correction factors can compensate for inaccurate values of these parameters. We give here guidelines, that can be used through tools within the iSMS software (http://www.isms.au.dk), for determining quantitative FRET and assess uncertainties linked with the procedure. Our results provide insights into the experimental parameters governing quantitative FRET determination, which is essential for obtaining accurate structural information from a wide range of biomolecules." @default.
• W1950929708 created "2016-06-24" @default.
• W1950929708 creator A5027564599 @default.
• W1950929708 creator A5047096246 @default.
• W1950929708 creator A5089938188 @default.
• W1950929708 date "2015-01-01" @default.
• W1950929708 modified "2023-09-27" @default.
• W1950929708 title "Quantitative single molecule FRET efficiencies using TIRF microscopy" @default.
• W1950929708 cites W1516180781 @default.
• W1950929708 cites W1957082099 @default.
• W1950929708 cites W1971172146 @default.
• W1950929708 cites W1972739518 @default.
• W1950929708 cites W1973699439 @default.
• W1950929708 cites W1981014781 @default.
• W1950929708 cites W1988741357 @default.
• W1950929708 cites W1996150709 @default.
• W1950929708 cites W2011940688 @default.
• W1950929708 cites W2045110889 @default.
• W1950929708 cites W2045155739 @default.
• W1950929708 cites W2051023684 @default.
• W1950929708 cites W2061334607 @default.
• W1950929708 cites W2105571876 @default.
• W1950929708 cites W2107834897 @default.
• W1950929708 cites W2113936160 @default.
• W1950929708 cites W2132480368 @default.
• W1950929708 cites W2132677630 @default.
• W1950929708 cites W2137410262 @default.
• W1950929708 cites W2142844453 @default.
• W1950929708 cites W2145325210 @default.
• W1950929708 cites W2150453750 @default.
• W1950929708 cites W2155581697 @default.
• W1950929708 cites W2162435350 @default.
• W1950929708 cites W2317301136 @default.
• W1950929708 cites W2329504830 @default.
• W1950929708 doi "https://doi.org/10.1039/c5fd00100e" @default.
• W1950929708 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26416760" @default.
• W1950929708 hasPublicationYear "2015" @default.
• W1950929708 type Work @default.
• W1950929708 sameAs 1950929708 @default.
• W1950929708 citedByCount "14" @default.
• W1950929708 countsByYear W19509297082016 @default.
• W1950929708 countsByYear W19509297082017 @default.
• W1950929708 countsByYear W19509297082018 @default.
• W1950929708 countsByYear W19509297082019 @default.
• W1950929708 countsByYear W19509297082021 @default.
• W1950929708 countsByYear W19509297082022 @default.
• W1950929708 crossrefType "journal-article" @default.
• W1950929708 hasAuthorship W1950929708A5027564599 @default.
• W1950929708 hasAuthorship W1950929708A5047096246 @default.
• W1950929708 hasAuthorship W1950929708A5089938188 @default.
• W1950929708 hasConcept C113196181 @default.
• W1950929708 hasConcept C120665830 @default.
• W1950929708 hasConcept C121332964 @default.
• W1950929708 hasConcept C121381206 @default.
• W1950929708 hasConcept C147080431 @default.
• W1950929708 hasConcept C159467904 @default.
• W1950929708 hasConcept C169274487 @default.
• W1950929708 hasConcept C171250308 @default.
• W1950929708 hasConcept C178790620 @default.
• W1950929708 hasConcept C185592680 @default.
• W1950929708 hasConcept C186060115 @default.
• W1950929708 hasConcept C192562407 @default.
• W1950929708 hasConcept C26873012 @default.
• W1950929708 hasConcept C2779892579 @default.
• W1950929708 hasConcept C32909587 @default.
• W1950929708 hasConcept C43617362 @default.
• W1950929708 hasConcept C49853544 @default.
• W1950929708 hasConcept C86803240 @default.
• W1950929708 hasConcept C91881484 @default.
• W1950929708 hasConcept C96305047 @default.
• W1950929708 hasConceptScore W1950929708C113196181 @default.
• W1950929708 hasConceptScore W1950929708C120665830 @default.
• W1950929708 hasConceptScore W1950929708C121332964 @default.
• W1950929708 hasConceptScore W1950929708C121381206 @default.
• W1950929708 hasConceptScore W1950929708C147080431 @default.
• W1950929708 hasConceptScore W1950929708C159467904 @default.
• W1950929708 hasConceptScore W1950929708C169274487 @default.
• W1950929708 hasConceptScore W1950929708C171250308 @default.
• W1950929708 hasConceptScore W1950929708C178790620 @default.
• W1950929708 hasConceptScore W1950929708C185592680 @default.
• W1950929708 hasConceptScore W1950929708C186060115 @default.
• W1950929708 hasConceptScore W1950929708C192562407 @default.
• W1950929708 hasConceptScore W1950929708C26873012 @default.
• W1950929708 hasConceptScore W1950929708C2779892579 @default.
• W1950929708 hasConceptScore W1950929708C32909587 @default.
• W1950929708 hasConceptScore W1950929708C43617362 @default.
• W1950929708 hasConceptScore W1950929708C49853544 @default.
• W1950929708 hasConceptScore W1950929708C86803240 @default.
• W1950929708 hasConceptScore W1950929708C91881484 @default.
• W1950929708 hasConceptScore W1950929708C96305047 @default.
• W1950929708 hasFunder F4320320943 @default.
• W1950929708 hasFunder F4320321446 @default.
• W1950929708 hasLocation W19509297081 @default.
• W1950929708 hasLocation W19509297082 @default.
• W1950929708 hasOpenAccess W1950929708 @default.
• W1950929708 hasPrimaryLocation W19509297081 @default.
• W1950929708 hasRelatedWork W1537873589 @default.
• W1950929708 hasRelatedWork W1554269112 @default.

• next