FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1951301958> ?p ?o ?g. }

• W1951301958 endingPage "851" @default.
• W1951301958 startingPage "844" @default.
• W1951301958 abstract "Abstract Background: Molecular detection methods for HER2/neu gene amplification include fluorescence in situ hybridization (FISH) and competitive PCR. We designed a quantitative PCR system utilizing fluorescent hybridization probes and a competitor that differed from the HER2/neu sequence by a single base change. Methods: Increasing twofold concentrations of competitor were coamplified with DNA from cell lines with various HER2/neu copy numbers at the HER2/neu locus. Competitor DNA was distinguished from the HER2/neu sequence by a fluorescent hybridization probe and melting curve analysis on a fluorescence-monitoring thermal cycler. The percentages of competitor to target peak areas on derivative fluorescence vs temperature curves were used to calculate copy number. Results: Real-time monitoring of the PCR reaction showed comparable relative areas throughout the log phase and during the PCR plateau, indicating that only end-point detection is necessary. The dynamic range was over two logs (2000–250 000 competitor copies) with CVs &lt;20%. Three cell lines (MRC-5, T-47D, and SK-BR-3) were determined to have gene doses of 1, 3, and 11, respectively. Gene amplification was detected in 3 of 13 tumor samples and was correlated with conventional real-time PCR and FISH analysis. Conclusion: Use of relative peak areas allows gene copy numbers to be quantified against an internal competitive control in &lt;1 h." @default.
• W1951301958 created "2016-06-24" @default.
• W1951301958 creator A5014144008 @default.
• W1951301958 creator A5026306864 @default.
• W1951301958 creator A5051507868 @default.
• W1951301958 creator A5053831167 @default.
• W1951301958 creator A5082018169 @default.
• W1951301958 date "2001-05-01" @default.
• W1951301958 modified "2023-10-16" @default.
• W1951301958 title "Quantification of HER2/neu Gene Amplification by Competitive PCR Using Fluorescent Melting Curve Analysis" @default.
• W1951301958 cites W1964023306 @default.
• W1951301958 cites W1964931822 @default.
• W1951301958 cites W1991267989 @default.
• W1951301958 cites W1991416943 @default.
• W1951301958 cites W1991847152 @default.
• W1951301958 cites W1995301858 @default.
• W1951301958 cites W2017953079 @default.
• W1951301958 cites W2022133483 @default.
• W1951301958 cites W2032366074 @default.
• W1951301958 cites W2050717506 @default.
• W1951301958 cites W2056324634 @default.
• W1951301958 cites W2060531515 @default.
• W1951301958 cites W2064499954 @default.
• W1951301958 cites W2070522604 @default.
• W1951301958 cites W2071164914 @default.
• W1951301958 cites W2085819983 @default.
• W1951301958 cites W2093009120 @default.
• W1951301958 cites W2095156669 @default.
• W1951301958 cites W2110612892 @default.
• W1951301958 cites W2113001043 @default.
• W1951301958 cites W2124268967 @default.
• W1951301958 cites W2143540259 @default.
• W1951301958 cites W2154027407 @default.
• W1951301958 cites W2171678787 @default.
• W1951301958 cites W4254533614 @default.
• W1951301958 doi "https://doi.org/10.1093/clinchem/47.5.844" @default.
• W1951301958 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11325887" @default.
• W1951301958 hasPublicationYear "2001" @default.
• W1951301958 type Work @default.
• W1951301958 sameAs 1951301958 @default.
• W1951301958 citedByCount "52" @default.
• W1951301958 countsByYear W19513019582012 @default.
• W1951301958 countsByYear W19513019582013 @default.
• W1951301958 countsByYear W19513019582014 @default.
• W1951301958 countsByYear W19513019582015 @default.
• W1951301958 countsByYear W19513019582017 @default.
• W1951301958 countsByYear W19513019582020 @default.
• W1951301958 countsByYear W19513019582022 @default.
• W1951301958 crossrefType "journal-article" @default.
• W1951301958 hasAuthorship W1951301958A5014144008 @default.
• W1951301958 hasAuthorship W1951301958A5026306864 @default.
• W1951301958 hasAuthorship W1951301958A5051507868 @default.
• W1951301958 hasAuthorship W1951301958A5053831167 @default.
• W1951301958 hasAuthorship W1951301958A5082018169 @default.
• W1951301958 hasBestOaLocation W19513019581 @default.
• W1951301958 hasConcept C104317684 @default.
• W1951301958 hasConcept C121332964 @default.
• W1951301958 hasConcept C153911025 @default.
• W1951301958 hasConcept C185592680 @default.
• W1951301958 hasConcept C2777542201 @default.
• W1951301958 hasConcept C2780968160 @default.
• W1951301958 hasConcept C30481170 @default.
• W1951301958 hasConcept C48023723 @default.
• W1951301958 hasConcept C49105822 @default.
• W1951301958 hasConcept C54355233 @default.
• W1951301958 hasConcept C552990157 @default.
• W1951301958 hasConcept C62520636 @default.
• W1951301958 hasConcept C7602840 @default.
• W1951301958 hasConcept C84133368 @default.
• W1951301958 hasConcept C84597430 @default.
• W1951301958 hasConcept C86803240 @default.
• W1951301958 hasConcept C91881484 @default.
• W1951301958 hasConceptScore W1951301958C104317684 @default.
• W1951301958 hasConceptScore W1951301958C121332964 @default.
• W1951301958 hasConceptScore W1951301958C153911025 @default.
• W1951301958 hasConceptScore W1951301958C185592680 @default.
• W1951301958 hasConceptScore W1951301958C2777542201 @default.
• W1951301958 hasConceptScore W1951301958C2780968160 @default.
• W1951301958 hasConceptScore W1951301958C30481170 @default.
• W1951301958 hasConceptScore W1951301958C48023723 @default.
• W1951301958 hasConceptScore W1951301958C49105822 @default.
• W1951301958 hasConceptScore W1951301958C54355233 @default.
• W1951301958 hasConceptScore W1951301958C552990157 @default.
• W1951301958 hasConceptScore W1951301958C62520636 @default.
• W1951301958 hasConceptScore W1951301958C7602840 @default.
• W1951301958 hasConceptScore W1951301958C84133368 @default.
• W1951301958 hasConceptScore W1951301958C84597430 @default.
• W1951301958 hasConceptScore W1951301958C86803240 @default.
• W1951301958 hasConceptScore W1951301958C91881484 @default.
• W1951301958 hasIssue "5" @default.
• W1951301958 hasLocation W19513019581 @default.
• W1951301958 hasLocation W19513019582 @default.
• W1951301958 hasOpenAccess W1951301958 @default.
• W1951301958 hasPrimaryLocation W19513019581 @default.
• W1951301958 hasRelatedWork W183055358 @default.
• W1951301958 hasRelatedWork W2020090364 @default.
• W1951301958 hasRelatedWork W2021067281 @default.
• W1951301958 hasRelatedWork W2055484162 @default.

• next