FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1951321216> ?p ?o ?g. }

• W1951321216 endingPage "171" @default.
• W1951321216 startingPage "162" @default.
• W1951321216 abstract "Dissimilatory nitrate reductase was purified to homogeneity from anaerobic cultures of the denitrifying bacterium Pseudomonas aeruginosa. The following procedures were used in the rapid isolation of this unstable enzyme: induction by nitrate in semianaerobic cell suspension, heat-stimulated activation and solubilization from the membrane fraction, and purification by hydrophobic interaction chromatography. The molecular weight of the purified enzyme was estimated by nondenaturing polyacrylamide gel electrophoresis, sucrose density gradient sedimentation, and gel filtration chromatography. Subunit molecular weights were estimated by electrophoresis in sodium dodecyl sulfate-polyacrylamide gels. The active enzyme monomer, with a molecular weight of 176,000 to 260,000 (depending upon the method of determination), was composed of subunits with molecular weights of approximately 64,000 and 118,000. The monomer aggregated to form an inactive tetramer of about 800,000 molecular weight. Purified enzyme exhibited a broad pH optimum, between 6.5 and 7.5. Kinetic studies showed that the apparent Km was 0.30 mM for nitrate, and 2.2 to 2.9 microM for dithionite-reduced benzyl viologen. Azide was an effective inhibitor: the concentration required for half-maximal inhibition was 21 to 24 microM. Azide inhibition was competitive with nitrate (Ki = 2.0 microM) but uncompetitive with reduced benzyl viologen (Ki = 25 microM). Based upon spectral evidence, the purified molybdo-enzyme had no associated cytochromes but did contain nonhaem iron that responded to dithionite reduction and nitrate oxidation. The enzyme that was purified after being heat solubilized from membranes had properties essentially identical to those of the enzyme that was purified after deoxycholate solubilization." @default.
• W1951321216 created "2016-06-24" @default.
• W1951321216 creator A5029805615 @default.
• W1951321216 creator A5047663307 @default.
• W1951321216 creator A5077239388 @default.
• W1951321216 date "1982-07-01" @default.
• W1951321216 modified "2023-09-25" @default.
• W1951321216 title "Properties of dissimilatory nitrate reductase purified from the denitrifier Pseudomonas aeruginosa" @default.
• W1951321216 cites W1011526260 @default.
• W1951321216 cites W129959766 @default.
• W1951321216 cites W1504528101 @default.
• W1951321216 cites W1506393506 @default.
• W1951321216 cites W1515430115 @default.
• W1951321216 cites W1529512777 @default.
• W1951321216 cites W1561046738 @default.
• W1951321216 cites W1566949129 @default.
• W1951321216 cites W1571130748 @default.
• W1951321216 cites W1572342911 @default.
• W1951321216 cites W15769450 @default.
• W1951321216 cites W1588135440 @default.
• W1951321216 cites W1600013467 @default.
• W1951321216 cites W1611482599 @default.
• W1951321216 cites W166506190 @default.
• W1951321216 cites W1775749144 @default.
• W1951321216 cites W1800618698 @default.
• W1951321216 cites W1860270028 @default.
• W1951321216 cites W1937415696 @default.
• W1951321216 cites W1959882277 @default.
• W1951321216 cites W1969591033 @default.
• W1951321216 cites W1977068968 @default.
• W1951321216 cites W1989744185 @default.
• W1951321216 cites W2000068302 @default.
• W1951321216 cites W2016471798 @default.
• W1951321216 cites W2030428485 @default.
• W1951321216 cites W2031643551 @default.
• W1951321216 cites W2034571172 @default.
• W1951321216 cites W2036326050 @default.
• W1951321216 cites W2038345597 @default.
• W1951321216 cites W2045799781 @default.
• W1951321216 cites W2050643839 @default.
• W1951321216 cites W2054979333 @default.
• W1951321216 cites W2071981508 @default.
• W1951321216 cites W2088402240 @default.
• W1951321216 cites W2101083328 @default.
• W1951321216 cites W2153201116 @default.
• W1951321216 cites W2210650111 @default.
• W1951321216 cites W2917777361 @default.
• W1951321216 cites W294831262 @default.
• W1951321216 cites W4248885647 @default.
• W1951321216 cites W56251836 @default.
• W1951321216 doi "https://doi.org/10.1128/jb.151.1.162-171.1982" @default.
• W1951321216 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/220222" @default.
• W1951321216 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6806238" @default.
• W1951321216 hasPublicationYear "1982" @default.
• W1951321216 type Work @default.
• W1951321216 sameAs 1951321216 @default.
• W1951321216 citedByCount "55" @default.
• W1951321216 countsByYear W19513212162012 @default.
• W1951321216 countsByYear W19513212162015 @default.
• W1951321216 countsByYear W19513212162017 @default.
• W1951321216 countsByYear W19513212162018 @default.
• W1951321216 countsByYear W19513212162019 @default.
• W1951321216 countsByYear W19513212162020 @default.
• W1951321216 countsByYear W19513212162023 @default.
• W1951321216 crossrefType "journal-article" @default.
• W1951321216 hasAuthorship W1951321216A5029805615 @default.
• W1951321216 hasAuthorship W1951321216A5047663307 @default.
• W1951321216 hasAuthorship W1951321216A5077239388 @default.
• W1951321216 hasBestOaLocation W19513212161 @default.
• W1951321216 hasConcept C101660505 @default.
• W1951321216 hasConcept C116084860 @default.
• W1951321216 hasConcept C14728290 @default.
• W1951321216 hasConcept C150757390 @default.
• W1951321216 hasConcept C169490005 @default.
• W1951321216 hasConcept C178790620 @default.
• W1951321216 hasConcept C181199279 @default.
• W1951321216 hasConcept C184651966 @default.
• W1951321216 hasConcept C185592680 @default.
• W1951321216 hasConcept C193363816 @default.
• W1951321216 hasConcept C2777315085 @default.
• W1951321216 hasConcept C2778710519 @default.
• W1951321216 hasConcept C2778913249 @default.
• W1951321216 hasConcept C2781417787 @default.
• W1951321216 hasConcept C28212737 @default.
• W1951321216 hasConcept C43617362 @default.
• W1951321216 hasConcept C55493867 @default.
• W1951321216 hasConcept C58121356 @default.
• W1951321216 hasConcept C86803240 @default.
• W1951321216 hasConceptScore W1951321216C101660505 @default.
• W1951321216 hasConceptScore W1951321216C116084860 @default.
• W1951321216 hasConceptScore W1951321216C14728290 @default.
• W1951321216 hasConceptScore W1951321216C150757390 @default.
• W1951321216 hasConceptScore W1951321216C169490005 @default.
• W1951321216 hasConceptScore W1951321216C178790620 @default.
• W1951321216 hasConceptScore W1951321216C181199279 @default.
• W1951321216 hasConceptScore W1951321216C184651966 @default.
• W1951321216 hasConceptScore W1951321216C185592680 @default.
• W1951321216 hasConceptScore W1951321216C193363816 @default.

• next