Matches in SemOpenAlex for { <https://semopenalex.org/work/W1952484212> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W1952484212 endingPage "789" @default.
- W1952484212 startingPage "776" @default.
- W1952484212 abstract "The NADPH-mediated metabolism of aflatoxin B1 by rat hepatic microsomes was studied by means of difference spectroscopy. A decrease in absorbance at 360 nm, due to the disappearance of aflatoxin B1 during its metabolism, was caused by the formation of a metabolite having a spectral peak at 398 nm. From the solubility, spectral, and microsomal binding characteristics, this metabolite appears to be aflatoxin B2a (aflatoxin hemiacetal). Difference spectroscopic studies on microsomes reisolated from incubation mixtures containing aflatoxin B1 with or without NADPH showed that aflatoxin B1 was bound to microsomes, giving a complex having a spectral peak at 360 nm; this binding did not require NADPH and the complex could be separated by gel filtration chromatography. A metabolite of aflatoxin B1, the formation of which was NADPH-dependent, was bound to microsomes, yielding a complex having a spectral peak at 405 nm; this complex was not dissociated by gel chromatography on by treatment with trichloracetic acid or extraction with chloroform and acetone. SKF 525-A and L-cysteine inhibited the formation of this complex, the former by inhibiting the metabolic conversion of aflatoxin B1 to aflatoxin B2a and the latter by blocking the binding of the metabolite to microsomes. Aflatoxin B1 and aflatoxin B2a appeared to bind to different sites on the microsomes. A change in pH caused an alteration in the structure of aflatoxin B2a and its extraction with chloroform. pH equilibria for this phenomenon were estimated to be 3.00 and 7.35. Based on these studies, it is proposed that aflatoxin B2a, under alkaline conditions, cleaves to yield a dialdehyde derivative(s) which binds to microsomes, forming Schiff bases with free amino groups. These studies also indicate that, under certain conditions, rat hepatic microsome-mediated conversion of aflatoxin B1 to a water-soluble metabolite, which appears to be aflatoxin B2a, represents a major metabolic pathway." @default.
- W1952484212 created "2016-06-24" @default.
- W1952484212 creator A5018257393 @default.
- W1952484212 creator A5059002976 @default.
- W1952484212 date "1974-09-01" @default.
- W1952484212 modified "2023-09-28" @default.
- W1952484212 title "Metabolism of Aflatoxin B1 and Its Metabolism-Dependent and Independent Binding to Rat Hepatic Microsomes" @default.
- W1952484212 hasPublicationYear "1974" @default.
- W1952484212 type Work @default.
- W1952484212 sameAs 1952484212 @default.
- W1952484212 citedByCount "9" @default.
- W1952484212 countsByYear W19524842122013 @default.
- W1952484212 crossrefType "journal-article" @default.
- W1952484212 hasAuthorship W1952484212A5018257393 @default.
- W1952484212 hasAuthorship W1952484212A5059002976 @default.
- W1952484212 hasConcept C181199279 @default.
- W1952484212 hasConcept C185592680 @default.
- W1952484212 hasConcept C2777477808 @default.
- W1952484212 hasConcept C2781144210 @default.
- W1952484212 hasConcept C31903555 @default.
- W1952484212 hasConcept C40758303 @default.
- W1952484212 hasConcept C43617362 @default.
- W1952484212 hasConcept C55493867 @default.
- W1952484212 hasConcept C62231903 @default.
- W1952484212 hasConcept C87644729 @default.
- W1952484212 hasConcept C98015330 @default.
- W1952484212 hasConceptScore W1952484212C181199279 @default.
- W1952484212 hasConceptScore W1952484212C185592680 @default.
- W1952484212 hasConceptScore W1952484212C2777477808 @default.
- W1952484212 hasConceptScore W1952484212C2781144210 @default.
- W1952484212 hasConceptScore W1952484212C31903555 @default.
- W1952484212 hasConceptScore W1952484212C40758303 @default.
- W1952484212 hasConceptScore W1952484212C43617362 @default.
- W1952484212 hasConceptScore W1952484212C55493867 @default.
- W1952484212 hasConceptScore W1952484212C62231903 @default.
- W1952484212 hasConceptScore W1952484212C87644729 @default.
- W1952484212 hasConceptScore W1952484212C98015330 @default.
- W1952484212 hasIssue "5" @default.
- W1952484212 hasLocation W19524842121 @default.
- W1952484212 hasOpenAccess W1952484212 @default.
- W1952484212 hasPrimaryLocation W19524842121 @default.
- W1952484212 hasRelatedWork W1775749144 @default.
- W1952484212 hasRelatedWork W1775964807 @default.
- W1952484212 hasRelatedWork W1831495162 @default.
- W1952484212 hasRelatedWork W1976798042 @default.
- W1952484212 hasRelatedWork W1976835752 @default.
- W1952484212 hasRelatedWork W1985281171 @default.
- W1952484212 hasRelatedWork W2001001513 @default.
- W1952484212 hasRelatedWork W2004241245 @default.
- W1952484212 hasRelatedWork W2031112211 @default.
- W1952484212 hasRelatedWork W2048770618 @default.
- W1952484212 hasRelatedWork W2053904061 @default.
- W1952484212 hasRelatedWork W2056326460 @default.
- W1952484212 hasRelatedWork W2059688422 @default.
- W1952484212 hasRelatedWork W2094102886 @default.
- W1952484212 hasRelatedWork W2108197722 @default.
- W1952484212 hasRelatedWork W2116164984 @default.
- W1952484212 hasRelatedWork W2120603617 @default.
- W1952484212 hasRelatedWork W2127361222 @default.
- W1952484212 hasRelatedWork W2135469641 @default.
- W1952484212 hasRelatedWork W2232615845 @default.
- W1952484212 hasVolume "10" @default.
- W1952484212 isParatext "false" @default.
- W1952484212 isRetracted "false" @default.
- W1952484212 magId "1952484212" @default.
- W1952484212 workType "article" @default.