FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1954397675> ?p ?o ?g. }

• W1954397675 endingPage "3222" @default.
• W1954397675 startingPage "3208" @default.
• W1954397675 abstract "Phosphorylation of the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2 alpha) impairs translation initiation by inhibiting the guanine nucleotide exchange factor for eIF-2, known as eIF-2B. In Saccharomyces cerevisiae, phosphorylation of eIF-2 alpha by the protein kinase GCN2 specifically stimulates translation of GCN4 mRNA in addition to reducing general protein synthesis. We isolated mutations in several unlinked genes that suppress the growth-inhibitory effect of eIF-2 alpha phosphorylation catalyzed by mutationally activated forms of GCN2. These suppressor mutations, affecting eIF-2 alpha and the essential subunits of eIF-2B encoded by GCD7 and GCD2, do not reduce the level of eIF-2 alpha phosphorylation in cells expressing the activated GCN2c kinase. Four GCD7 suppressors were shown to reduce the derepression of GCN4 translation in cells containing wild-type GCN2 under starvation conditions or in GCN2c strains. A fifth GCD7 allele, constructed in vitro by combining two of the GCD7 suppressors mutations, completely impaired the derepression of GCN4 translation, a phenotype characteristic of deletions in GCN1, GCN2, or GCN3. This double GCD7 mutation also completely suppressed the lethal effect of expressing the mammalian eIF-2 alpha kinase dsRNA-PK in yeast cells, showing that the translational machinery had been rendered completely insensitive to phosphorylated eIF-2. None of the GCD7 mutations had any detrimental effect on cell growth under nonstarvation conditions, suggesting that recycling of eIF-2 occurs efficiently in the suppressor strains. We propose that GCD7 and GCD2 play important roles in the regulatory interaction between eIF-2 and eIF-2B and that the suppressor mutations we isolated in these genes decrease the susceptibility of eIF-2B to the inhibitory effects of phosphorylated eIF-2 without impairing the essential catalytic function of eIF-2B in translation initiation." @default.
• W1954397675 created "2016-06-24" @default.
• W1954397675 creator A5019704848 @default.
• W1954397675 creator A5087079106 @default.
• W1954397675 date "1994-05-01" @default.
• W1954397675 modified "2023-10-14" @default.
• W1954397675 title "Mutations in the GCD7 subunit of yeast guanine nucleotide exchange factor eIF-2B overcome the inhibitory effects of phosphorylated eIF-2 on translation initiation." @default.
• W1954397675 cites W1488906799 @default.
• W1954397675 cites W1555701100 @default.
• W1954397675 cites W1677962389 @default.
• W1954397675 cites W1758999400 @default.
• W1954397675 cites W1769175751 @default.
• W1954397675 cites W179070074 @default.
• W1954397675 cites W1792328490 @default.
• W1954397675 cites W1817433838 @default.
• W1954397675 cites W1820624545 @default.
• W1954397675 cites W1856116921 @default.
• W1954397675 cites W1882558703 @default.
• W1954397675 cites W1886701353 @default.
• W1954397675 cites W1896726814 @default.
• W1954397675 cites W1899601083 @default.
• W1954397675 cites W1902596355 @default.
• W1954397675 cites W1908579120 @default.
• W1954397675 cites W1965017130 @default.
• W1954397675 cites W1965177762 @default.
• W1954397675 cites W1968727721 @default.
• W1954397675 cites W1969107860 @default.
• W1954397675 cites W1973782344 @default.
• W1954397675 cites W1974129290 @default.
• W1954397675 cites W1979202944 @default.
• W1954397675 cites W1990344643 @default.
• W1954397675 cites W2036853011 @default.
• W1954397675 cites W2037724550 @default.
• W1954397675 cites W2047662834 @default.
• W1954397675 cites W2050611086 @default.
• W1954397675 cites W2055323371 @default.
• W1954397675 cites W2097687129 @default.
• W1954397675 cites W2097864205 @default.
• W1954397675 cites W2110786863 @default.
• W1954397675 cites W2112836983 @default.
• W1954397675 cites W2117348012 @default.
• W1954397675 cites W2138270253 @default.
• W1954397675 cites W2141606719 @default.
• W1954397675 cites W2144879264 @default.
• W1954397675 cites W2165055107 @default.
• W1954397675 cites W2166661434 @default.
• W1954397675 cites W2169333620 @default.
• W1954397675 cites W2169465564 @default.
• W1954397675 cites W218318767 @default.
• W1954397675 cites W2254141979 @default.
• W1954397675 cites W2337385015 @default.
• W1954397675 cites W2596796917 @default.
• W1954397675 cites W72118176 @default.
• W1954397675 cites W955649814 @default.
• W1954397675 doi "https://doi.org/10.1128/mcb.14.5.3208" @default.
• W1954397675 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/358688" @default.
• W1954397675 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8164676" @default.
• W1954397675 hasPublicationYear "1994" @default.
• W1954397675 type Work @default.
• W1954397675 sameAs 1954397675 @default.
• W1954397675 citedByCount "48" @default.
• W1954397675 countsByYear W19543976752013 @default.
• W1954397675 countsByYear W19543976752015 @default.
• W1954397675 countsByYear W19543976752016 @default.
• W1954397675 countsByYear W19543976752017 @default.
• W1954397675 countsByYear W19543976752018 @default.
• W1954397675 countsByYear W19543976752019 @default.
• W1954397675 countsByYear W19543976752020 @default.
• W1954397675 countsByYear W19543976752021 @default.
• W1954397675 crossrefType "journal-article" @default.
• W1954397675 hasAuthorship W1954397675A5019704848 @default.
• W1954397675 hasAuthorship W1954397675A5087079106 @default.
• W1954397675 hasBestOaLocation W19543976751 @default.
• W1954397675 hasConcept C104292427 @default.
• W1954397675 hasConcept C104317684 @default.
• W1954397675 hasConcept C105580179 @default.
• W1954397675 hasConcept C11960822 @default.
• W1954397675 hasConcept C126619667 @default.
• W1954397675 hasConcept C149364088 @default.
• W1954397675 hasConcept C150194340 @default.
• W1954397675 hasConcept C153911025 @default.
• W1954397675 hasConcept C186310378 @default.
• W1954397675 hasConcept C2777576037 @default.
• W1954397675 hasConcept C4718897 @default.
• W1954397675 hasConcept C55493867 @default.
• W1954397675 hasConcept C62478195 @default.
• W1954397675 hasConcept C62493599 @default.
• W1954397675 hasConcept C73766848 @default.
• W1954397675 hasConcept C83730767 @default.
• W1954397675 hasConcept C86803240 @default.
• W1954397675 hasConcept C93734116 @default.
• W1954397675 hasConcept C95444343 @default.
• W1954397675 hasConcept C97029542 @default.
• W1954397675 hasConceptScore W1954397675C104292427 @default.
• W1954397675 hasConceptScore W1954397675C104317684 @default.
• W1954397675 hasConceptScore W1954397675C105580179 @default.
• W1954397675 hasConceptScore W1954397675C11960822 @default.
• W1954397675 hasConceptScore W1954397675C126619667 @default.

• next