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- W1954728901 abstract "Abstract An optimized assay for alkaline phosphatase (EC 3.1.3.1) is reported. A discrete analyzer, the DuPont Automatic Clinical Analyzer (aca), was used for this study. The assay is based on results of response-surface experimental co-optimization techniques, and response is enhanced over the present aca assay. A key feature is the incorporation of zinc ions, both to fully optimize the assay and to reduce the sensitivity of measured activity to zinc-binding impurities in the buffer, 2-amino-2-methyl-1-propanol. In addition, a simple technique is described for measuring relative concentrations of zinc-binding impurities in this buffer. These features should be considered in the design of any assay for alkaline phosphatase that is based on p-nitrophenyl phosphate as substrate and 2-amino-2-methyl-1-propanol as buffer." @default.
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- W1954728901 date "1981-07-01" @default.
- W1954728901 modified "2023-09-24" @default.
- W1954728901 title "Response-surface-optimized, zinc-enhanced assay for serum alkaline phosphatase." @default.
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- W1954728901 doi "https://doi.org/10.1093/clinchem/27.7.1171" @default.
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