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• W1955369670 startingPage "4389" @default.
• W1955369670 abstract "We have studied transcription elongation and termination in the human c-myc gene. Transcription of c-myc gene sequences with purified mammalian RNA polymerase II revealed several sites of transcription termination and pausing in the vicinity of the exon 1-intron 1 junction. This region previously has been shown to block transcription elongation in vivo by nuclear run-on analysis (D. Bentley and M. Groudine, Nature [London] 321:702-706, 1986). These sites were recognized by purified RNA polymerase II, and we therefore designated them intrinsic sites of termination and pausing. Two of these sites cause termination of RNA polymerase III transcription as well. RNA polymerase II terminated transcription in a cluster of seven consecutive T residues in the nontranscribed strand and paused during transcription at three additional sites in this region. The intrinsic sites of transcription termination and pausing described here correspond closely to the 3' ends of transcripts synthesized in Xenopus oocytes injected with plasmids containing the c-myc termination region (D. Bentley and M. Groudine, Cell 53:245-256, 1988). This correspondence suggests that the intrinsic recognition of these termination and pause sites by purified RNA polymerase II may play a role in the transcription elongation block observed in vivo." @default.
• W1955369670 created "2016-06-24" @default.
• W1955369670 creator A5015185317 @default.
• W1955369670 creator A5047184482 @default.
• W1955369670 date "1988-10-01" @default.
• W1955369670 modified "2023-10-13" @default.
• W1955369670 title "Intrinsic sites of transcription termination and pausing in the c-myc gene." @default.
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• W1955369670 doi "https://doi.org/10.1128/mcb.8.10.4389" @default.
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