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• W1955900143 abstract "Consumption of ochratoxin A (OTA) contaminated diets by broilers results in economic losses to the poultry industry. This experiment evaluated the effects of quantity and time of exposure to dietary OTA on performance, organ weights, serum biochemistry, and renal gene expression of chicks. Determination of genes expressed in response to OTA will allow for the identification of pathways that are influenced by OTA. 180-day old male broiler chicks were randomly assigned to a 3×3 factorial arrangement of treatments (3 levels of OTA; 0, 1 and 2 mg OTA/kg diet and 3 time periods; 7, 14 and 21 days) with 4 replicate pens of 5 birds each per treatment. For RNA-sequencing analysis (RNA-Seq), kidney samples were collected weekly from 3 controls and 3 chicks fed 1 mg OTA/kg. NextGENe software was used for read alignment and transcript quantification. Birds fed 2 mg OTA/kg diet had decreased feed intake and body weight gain, and increased serum uric acid on days 14 and 21. Compared to controls, birds fed 2 mg OTA/kg diet also had poorer feed conversion and increased kidney weights. On day 21, birds fed 1 mg OTA/kg diet had decreased albumin, and aspartate aminotransferase concentrations. Genes associated with carbohydrate and amino acid metabolism were downregulated, and genes associated with the immune system were upregulated at days 7 and 14. Genes associated with lipid metabolism and xenobiotic biodegradation were also downregulated on day 14. These changes disappeared on day 21 suggesting that the kidney and other related organs were repaired or the damage was contained. In conclusion, decreased performance and increased kidney weight and serum uric acid in birds fed 2 mg OTA/kg confirmed the effects of OTA. Supplementation of 1 mg OTA/kg diet caused time-dependent alterations in renal gene expression in chicks." @default.
• W1955900143 created "2016-06-24" @default.
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• W1955900143 date "2016-03-11" @default.
• W1955900143 modified "2023-09-30" @default.
• W1955900143 title "Gene expression in the kidneys of broilers fed ochratoxin A for different time periods" @default.
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• W1955900143 doi "https://doi.org/10.3920/wmj2014.1873" @default.
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