FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1957057114> ?p ?o ?g. }

• W1957057114 endingPage "1172" @default.
• W1957057114 startingPage "1163" @default.
• W1957057114 abstract "We have studied the organization of clathrin during its transport in axons. Using immunoprecipitation techniques we have confirmed earlier findings that clathrin is transported as part of slow component b, but we also detect small amounts of clathrin in fast component. As fast component is known to correspond to the transport of membraneous material, including coated vesicle membrane components, our findings suggest that some clathrin in axons undergoes transport in the form of coated membranes and that a portion of the clathrin delivered to axons and axon terminals arrives by way of fast component. The organizational form of clathrin in slow component b (SCb) was examined in more detail, as it is thought to represent a non-membrane-associated species, is relatively long-lived, and at any instant represents the major transport species in axons. We used nondenaturing immunoprecipitation methods with stringent wash procedures to identify other SCb proteins that interact with clathrin. The immunoprecipitates contained major labeled bands that corresponded to clathrin heavy and light chains, along with a prominent 70-kDa band and several minor bands that ranged in apparent Mr from 70,000 to 150,000; the 70-kDa band was shown to be the ATP-dependent uncoating protein by two-dimensional gel electrophoresis. A very similar profile of polypeptides was also immunoprecipitated from extracts of cultured neurons. The results from a variety of control immunoprecipitations, including the use of antisera preadsorbed with purified clathrin trimers or clathrin light chains, indicate that coprecipitation of clathrin and uncoating protein with the other 70,000-150,000-Da polypeptides from SCb reflects specific interactions. Including exogenous uncoating protein in the lysis buffer had no detectable effect on the levels of endogenous uncoating protein recovered in the immunoprecipitates, indicating that complexes of clathrin, uncoating protein, and the other coimmunoprecipitating SCb protein existed in the intact neurons prior to lysis. Finally, a specific and functional association is further supported by the release of uncoating protein, but not the other 70,000-150,000-Da polypeptides, from the immunoprecipitated complexes on the addition of ATP. Collectively, these observations provide the first direct evidence of interaction between clathrin and uncoating protein in intact cells, lend strong support to the concept that uncoating protein plays an intimate role in clathrin dynamics within cells, and reveal a family of 70,000-150,000-Da polypeptides that form a stable nonmembranous association with clathrin in intact cells." @default.
• W1957057114 created "2016-06-24" @default.
• W1957057114 creator A5046104849 @default.
• W1957057114 creator A5086707499 @default.
• W1957057114 creator A5090661120 @default.
• W1957057114 creator A5091458019 @default.
• W1957057114 date "1991-05-01" @default.
• W1957057114 modified "2023-10-10" @default.
• W1957057114 title "Stable clathrin: uncoating protein (hsc70) complexes in intact neurons and their axonal transport" @default.
• W1957057114 cites W1231809930 @default.
• W1957057114 cites W1492987806 @default.
• W1957057114 cites W1499469216 @default.
• W1957057114 cites W1508471581 @default.
• W1957057114 cites W1539678880 @default.
• W1957057114 cites W1562843589 @default.
• W1957057114 cites W1583927287 @default.
• W1957057114 cites W1591219300 @default.
• W1957057114 cites W1593791512 @default.
• W1957057114 cites W1710465380 @default.
• W1957057114 cites W1774185125 @default.
• W1957057114 cites W1958996074 @default.
• W1957057114 cites W1964238319 @default.
• W1957057114 cites W1968155837 @default.
• W1957057114 cites W1971800526 @default.
• W1957057114 cites W1974039139 @default.
• W1957057114 cites W1977388184 @default.
• W1957057114 cites W1981767347 @default.
• W1957057114 cites W1985820174 @default.
• W1957057114 cites W1989526931 @default.
• W1957057114 cites W1992888811 @default.
• W1957057114 cites W1992968802 @default.
• W1957057114 cites W2005383788 @default.
• W1957057114 cites W2017777380 @default.
• W1957057114 cites W2022125828 @default.
• W1957057114 cites W2023966699 @default.
• W1957057114 cites W2028724393 @default.
• W1957057114 cites W2034277598 @default.
• W1957057114 cites W2035198218 @default.
• W1957057114 cites W2050222635 @default.
• W1957057114 cites W2051643639 @default.
• W1957057114 cites W2051811506 @default.
• W1957057114 cites W2061226862 @default.
• W1957057114 cites W2061313613 @default.
• W1957057114 cites W2063270168 @default.
• W1957057114 cites W2067612885 @default.
• W1957057114 cites W2067708969 @default.
• W1957057114 cites W2069177009 @default.
• W1957057114 cites W2070616723 @default.
• W1957057114 cites W2070692986 @default.
• W1957057114 cites W2076258647 @default.
• W1957057114 cites W2076768997 @default.
• W1957057114 cites W2079442537 @default.
• W1957057114 cites W2079563569 @default.
• W1957057114 cites W2092198909 @default.
• W1957057114 cites W2098912888 @default.
• W1957057114 cites W2100285115 @default.
• W1957057114 cites W2102054712 @default.
• W1957057114 cites W2107245671 @default.
• W1957057114 cites W2107853353 @default.
• W1957057114 cites W2115397055 @default.
• W1957057114 cites W2121921106 @default.
• W1957057114 cites W2123359809 @default.
• W1957057114 cites W2129896183 @default.
• W1957057114 cites W2138466097 @default.
• W1957057114 cites W2154922728 @default.
• W1957057114 cites W2172095199 @default.
• W1957057114 cites W2217609893 @default.
• W1957057114 cites W237413193 @default.
• W1957057114 doi "https://doi.org/10.1523/jneurosci.11-05-01163.1991" @default.
• W1957057114 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6575316" @default.
• W1957057114 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1709204" @default.
• W1957057114 hasPublicationYear "1991" @default.
• W1957057114 type Work @default.
• W1957057114 sameAs 1957057114 @default.
• W1957057114 citedByCount "54" @default.
• W1957057114 countsByYear W19570571142012 @default.
• W1957057114 countsByYear W19570571142013 @default.
• W1957057114 countsByYear W19570571142016 @default.
• W1957057114 countsByYear W19570571142017 @default.
• W1957057114 countsByYear W19570571142020 @default.
• W1957057114 countsByYear W19570571142021 @default.
• W1957057114 crossrefType "journal-article" @default.
• W1957057114 hasAuthorship W1957057114A5046104849 @default.
• W1957057114 hasAuthorship W1957057114A5086707499 @default.
• W1957057114 hasAuthorship W1957057114A5090661120 @default.
• W1957057114 hasAuthorship W1957057114A5091458019 @default.
• W1957057114 hasBestOaLocation W19570571141 @default.
• W1957057114 hasConcept C104317684 @default.
• W1957057114 hasConcept C12554922 @default.
• W1957057114 hasConcept C130316041 @default.
• W1957057114 hasConcept C185592680 @default.
• W1957057114 hasConcept C41625074 @default.
• W1957057114 hasConcept C55493867 @default.
• W1957057114 hasConcept C63162447 @default.
• W1957057114 hasConcept C70192387 @default.
• W1957057114 hasConcept C71829478 @default.
• W1957057114 hasConcept C86803240 @default.
• W1957057114 hasConcept C95444343 @default.

• next