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- W1957234801 abstract "Summary The objective of the current study was to identify an optimal time period for donor cell transplantation after irradiation in sheep. The testes of recipient rams were treated with a single dose of 15 Gray (Gy) irradiation followed by germ cell transplantation either 3 or 6 weeks later. Transplantation of donor cells at 6 weeks after irradiation resulted in production of donor sperm by all five recipient rams compared with 4 of 11 rams transplanted at 3 weeks. Rams transplanted 3 weeks post‐irradiation appeared to show reduced libido and fertility. Two rams produced sperm with low motility (<20%) and two other rams were azoospermic. More than 1 year after cell transfer, there were heavy infiltrates of CD45‐positive cells and more fibrous tissue in 9 of 14 recipient testes (seven rams) that received cells 3 weeks after irradiation. Taken together, these results suggest that the interval between irradiation of recipients and germ cell transplantation affects the success rate of the procedure, with a 6‐week interval preferable. The elevated inflammatory/immune reaction may be responsible, at least in part, for the reduced fertility and low libido observed in the rams that received cells 3 weeks post‐irradiation." @default.
- W1957234801 created "2016-06-24" @default.
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- W1957234801 date "2010-09-21" @default.
- W1957234801 modified "2023-10-16" @default.
- W1957234801 title "A shorter interval between irradiation of recipient testis and germ cell transplantation is detrimental to recovery of fertility in rams" @default.
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- W1957234801 doi "https://doi.org/10.1111/j.1365-2605.2010.01113.x" @default.
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