FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1957238986> ?p ?o ?g. }

• W1957238986 abstract "Increased activity of B-Raf has been identified in approximately 7% of human cancers. Treatment of Eker rats (Tsc-2(EK/+) ), bearing a mutation in one allele of the tuberous sclerosis-2 (Tsc-2) gene, with the nephrocarcinogen 2,3,5-tris-(glutathion-S-yl) hydroquinone (TGHQ) results in loss of the wild-type allele of Tsc-2 in renal preneoplastic lesions and tumors. These tumors have increased protein expression of B-Raf, C-Raf (Raf-1), and increased expression and activity of ERK kinase. Similar changes are observed in Raf kinases following TGHQ-mediated transformation of primary renal epithelial cells derived from Tsc-2(EK/+) rats (QTRRE cells), cells that are also null for tuberin. Herein, we utilized LC-MS/MS to identify constitutive phosphorylation of S345 and S483 in both 100- and 95-kDa forms of B-Raf in QTRRE cells. Using microRotofor liquid-phase isoelectric focusing, we identified four fractions of B-Raf that contain different post-translational modification profiles in QTRRE cells. Amplification of the kinase domain of B-Raf from QTRRE cells, outer-stripe of the outer medulla of 8-month TGHQ- or vehicle-treated Tsc-2(+/+) and Tsc-2(EK/+) rats, as well as tumors excised from 8-month TGHQ-treated Tsc-2(EK/+) rats revealed three splice variants of B-Raf within the kinase domain. These splice variants differed by approximately 340, 544, and 600 bp; confirmed by sequencing. No point mutations within the kinase domain of B-Raf were identified. In addition, B-Raf/Raf-1/14-3-3 complex formation in the QTRRE cells was decreased by sorafenib, with concomitant selective decreases in p-ERK levels. Transcriptional and post-translational characterization of critical kinases, such as B-Raf, may contribute to the progression of tuberous sclerosis RCC. (246/250) © 2015 Wiley Periodicals, Inc." @default.
• W1957238986 created "2016-06-24" @default.
• W1957238986 creator A5027773175 @default.
• W1957238986 creator A5028417452 @default.
• W1957238986 creator A5059967974 @default.
• W1957238986 creator A5060909286 @default.
• W1957238986 creator A5077421484 @default.
• W1957238986 creator A5086522629 @default.
• W1957238986 date "2015-08-31" @default.
• W1957238986 modified "2023-10-17" @default.
• W1957238986 title "Transcriptional and post-translational modifications of B-Raf in quinol-thioether induced tuberous sclerosis renal cell carcinoma" @default.
• W1957238986 cites W134223655 @default.
• W1957238986 cites W1514408827 @default.
• W1957238986 cites W1516437155 @default.
• W1957238986 cites W1578042005 @default.
• W1957238986 cites W1815358876 @default.
• W1957238986 cites W1975131304 @default.
• W1957238986 cites W1978168320 @default.
• W1957238986 cites W1978654607 @default.
• W1957238986 cites W1979013732 @default.
• W1957238986 cites W2005254421 @default.
• W1957238986 cites W2007764966 @default.
• W1957238986 cites W2009713859 @default.
• W1957238986 cites W2017254121 @default.
• W1957238986 cites W2021208403 @default.
• W1957238986 cites W2038733728 @default.
• W1957238986 cites W2047227933 @default.
• W1957238986 cites W2050689883 @default.
• W1957238986 cites W2066278140 @default.
• W1957238986 cites W2074089133 @default.
• W1957238986 cites W2075082022 @default.
• W1957238986 cites W2096043174 @default.
• W1957238986 cites W2102646127 @default.
• W1957238986 cites W2105027798 @default.
• W1957238986 cites W2110487056 @default.
• W1957238986 cites W2123551753 @default.
• W1957238986 cites W2124662474 @default.
• W1957238986 cites W2125103653 @default.
• W1957238986 cites W2129202013 @default.
• W1957238986 cites W2139550352 @default.
• W1957238986 cites W2146946054 @default.
• W1957238986 cites W2160304102 @default.
• W1957238986 cites W2163188200 @default.
• W1957238986 cites W2328854759 @default.
• W1957238986 cites W2398899419 @default.
• W1957238986 cites W2413006958 @default.
• W1957238986 cites W4249817086 @default.
• W1957238986 cites W4290200018 @default.
• W1957238986 cites W80055403 @default.
• W1957238986 doi "https://doi.org/10.1002/mc.22366" @default.
• W1957238986 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4775466" @default.
• W1957238986 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26333016" @default.
• W1957238986 hasPublicationYear "2015" @default.
• W1957238986 type Work @default.
• W1957238986 sameAs 1957238986 @default.
• W1957238986 citedByCount "0" @default.
• W1957238986 crossrefType "journal-article" @default.
• W1957238986 hasAuthorship W1957238986A5027773175 @default.
• W1957238986 hasAuthorship W1957238986A5028417452 @default.
• W1957238986 hasAuthorship W1957238986A5059967974 @default.
• W1957238986 hasAuthorship W1957238986A5060909286 @default.
• W1957238986 hasAuthorship W1957238986A5077421484 @default.
• W1957238986 hasAuthorship W1957238986A5086522629 @default.
• W1957238986 hasBestOaLocation W19572389862 @default.
• W1957238986 hasConcept C104317684 @default.
• W1957238986 hasConcept C142724271 @default.
• W1957238986 hasConcept C143065580 @default.
• W1957238986 hasConcept C153911025 @default.
• W1957238986 hasConcept C176944494 @default.
• W1957238986 hasConcept C184235292 @default.
• W1957238986 hasConcept C2778980267 @default.
• W1957238986 hasConcept C32619005 @default.
• W1957238986 hasConcept C501734568 @default.
• W1957238986 hasConcept C502942594 @default.
• W1957238986 hasConcept C53345823 @default.
• W1957238986 hasConcept C55493867 @default.
• W1957238986 hasConcept C71924100 @default.
• W1957238986 hasConcept C86803240 @default.
• W1957238986 hasConcept C95444343 @default.
• W1957238986 hasConcept C97029542 @default.
• W1957238986 hasConceptScore W1957238986C104317684 @default.
• W1957238986 hasConceptScore W1957238986C142724271 @default.
• W1957238986 hasConceptScore W1957238986C143065580 @default.
• W1957238986 hasConceptScore W1957238986C153911025 @default.
• W1957238986 hasConceptScore W1957238986C176944494 @default.
• W1957238986 hasConceptScore W1957238986C184235292 @default.
• W1957238986 hasConceptScore W1957238986C2778980267 @default.
• W1957238986 hasConceptScore W1957238986C32619005 @default.
• W1957238986 hasConceptScore W1957238986C501734568 @default.
• W1957238986 hasConceptScore W1957238986C502942594 @default.
• W1957238986 hasConceptScore W1957238986C53345823 @default.
• W1957238986 hasConceptScore W1957238986C55493867 @default.
• W1957238986 hasConceptScore W1957238986C71924100 @default.
• W1957238986 hasConceptScore W1957238986C86803240 @default.
• W1957238986 hasConceptScore W1957238986C95444343 @default.
• W1957238986 hasConceptScore W1957238986C97029542 @default.
• W1957238986 hasFunder F4320332161 @default.
• W1957238986 hasFunder F4320337361 @default.
• W1957238986 hasLocation W19572389861 @default.
• W1957238986 hasLocation W19572389862 @default.

• next