FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W195739996> ?p ?o ?g. }

• W195739996 abstract "Caveolae (little caves) are 50 -100 nm invaginations seen on the plasma membrane of most cells. Caveolin-1, the marker protein of caveolae in most tissues, is the structural and functional unit of caveolae. Previous studies have demonstrated the tumor suppressor capability of caveolin-1. Our lab has already shown that expression of caveolin-1 induces premature senescence in cells, possibly including those with malignant potential. We proposed that this might explain the tumor suppressor function of caveolin-1. We have further shown that oxidative stress induces premature senescence through up regulation of caveolin-1. To further elucidate the molecular mechanisms underlying this process, we used H2O2 as a model to generate stress induced premature senescence (SIPS) and examined the response of caveolin-1 promoter under these conditions. Constructs with serially truncated segments of the mouse caveolin-1 gene promoter linked to a luciferase reporter gene were made and luciferase assays were carried out. These experiments demonstrated that the critical regions lay in two segments: -222/-372 and -91/-150 of the promoter region. A consensus Sp1 binding sequence was identified within each deleted segment. Gel shift analysis of protein binding from nuclear extracts to these caveolin promoter DNA sequences confirmed that transcription factors were binding to the Sp1 consensus elements as part of the transcriptional response to H2O2 induced senescence. Further deletion mutagenesis of the individual Sp1 consensus sites confirmed the identity of the transcription factor to be Sp1. These findings suggest that Sp1 mediates oxidant induced up regulation of caveolin-1 expression. In subsequent experiments, we examined the effect of inhibitors of p38 Mitogen activated protein kinase pathway on the levels of caveolin-1 expression during SIPS by western blot and luciferase assay. We found that this pathway plays a direct role in the up regulation of caveolin-1 during SIPS, possibly through modification of Sp1 to increase its activity.Public Health Importance: Cancer is a leading public health concern. Cav-1 has been shown to be a tumor suppressor gene involved in a large number of human tumors. Induction of premature senescence in cells with malignant potential is thought to be a vital tumor suppressor function. Our study aims to define the tumor suppressor capacity of Cav-1 by elucidating the pathway by which it induces premature senescence." @default.
• W195739996 created "2016-06-24" @default.
• W195739996 creator A5037769082 @default.
• W195739996 date "2005-07-08" @default.
• W195739996 modified "2023-09-27" @default.
• W195739996 title "Up regulation of caveolin-1 during H2O2 induced oxidative stress" @default.
• W195739996 cites W1549194476 @default.
• W195739996 cites W1982647833 @default.
• W195739996 cites W1987653805 @default.
• W195739996 cites W1991655565 @default.
• W195739996 cites W1995150314 @default.
• W195739996 cites W2003625629 @default.
• W195739996 cites W2023179617 @default.
• W195739996 cites W2032731042 @default.
• W195739996 cites W2034184306 @default.
• W195739996 cites W2044053783 @default.
• W195739996 cites W2053628284 @default.
• W195739996 cites W2056564646 @default.
• W195739996 cites W2059895279 @default.
• W195739996 cites W2061656219 @default.
• W195739996 cites W2068418997 @default.
• W195739996 cites W2072088194 @default.
• W195739996 cites W2080238905 @default.
• W195739996 cites W2094162221 @default.
• W195739996 cites W2097794242 @default.
• W195739996 cites W2110453766 @default.
• W195739996 cites W2111833940 @default.
• W195739996 cites W2144099695 @default.
• W195739996 cites W2144760773 @default.
• W195739996 cites W2170036297 @default.
• W195739996 hasPublicationYear "2005" @default.
• W195739996 type Work @default.
• W195739996 sameAs 195739996 @default.
• W195739996 citedByCount "0" @default.
• W195739996 crossrefType "journal-article" @default.
• W195739996 hasAuthorship W195739996A5037769082 @default.
• W195739996 hasConcept C101762097 @default.
• W195739996 hasConcept C104317684 @default.
• W195739996 hasConcept C111335760 @default.
• W195739996 hasConcept C138885662 @default.
• W195739996 hasConcept C150194340 @default.
• W195739996 hasConcept C153911025 @default.
• W195739996 hasConcept C179926584 @default.
• W195739996 hasConcept C24284526 @default.
• W195739996 hasConcept C27153228 @default.
• W195739996 hasConcept C2776410257 @default.
• W195739996 hasConcept C2779403975 @default.
• W195739996 hasConcept C2780870201 @default.
• W195739996 hasConcept C41895202 @default.
• W195739996 hasConcept C49866891 @default.
• W195739996 hasConcept C522857546 @default.
• W195739996 hasConcept C54009773 @default.
• W195739996 hasConcept C54355233 @default.
• W195739996 hasConcept C57467964 @default.
• W195739996 hasConcept C62478195 @default.
• W195739996 hasConcept C86339819 @default.
• W195739996 hasConcept C86803240 @default.
• W195739996 hasConcept C95444343 @default.
• W195739996 hasConceptScore W195739996C101762097 @default.
• W195739996 hasConceptScore W195739996C104317684 @default.
• W195739996 hasConceptScore W195739996C111335760 @default.
• W195739996 hasConceptScore W195739996C138885662 @default.
• W195739996 hasConceptScore W195739996C150194340 @default.
• W195739996 hasConceptScore W195739996C153911025 @default.
• W195739996 hasConceptScore W195739996C179926584 @default.
• W195739996 hasConceptScore W195739996C24284526 @default.
• W195739996 hasConceptScore W195739996C27153228 @default.
• W195739996 hasConceptScore W195739996C2776410257 @default.
• W195739996 hasConceptScore W195739996C2779403975 @default.
• W195739996 hasConceptScore W195739996C2780870201 @default.
• W195739996 hasConceptScore W195739996C41895202 @default.
• W195739996 hasConceptScore W195739996C49866891 @default.
• W195739996 hasConceptScore W195739996C522857546 @default.
• W195739996 hasConceptScore W195739996C54009773 @default.
• W195739996 hasConceptScore W195739996C54355233 @default.
• W195739996 hasConceptScore W195739996C57467964 @default.
• W195739996 hasConceptScore W195739996C62478195 @default.
• W195739996 hasConceptScore W195739996C86339819 @default.
• W195739996 hasConceptScore W195739996C86803240 @default.
• W195739996 hasConceptScore W195739996C95444343 @default.
• W195739996 hasLocation W1957399961 @default.
• W195739996 hasOpenAccess W195739996 @default.
• W195739996 hasPrimaryLocation W1957399961 @default.
• W195739996 hasRelatedWork W1964224010 @default.
• W195739996 hasRelatedWork W2027011895 @default.
• W195739996 hasRelatedWork W2029475270 @default.
• W195739996 hasRelatedWork W2041213381 @default.
• W195739996 hasRelatedWork W2050381846 @default.
• W195739996 hasRelatedWork W2058720888 @default.
• W195739996 hasRelatedWork W2062354480 @default.
• W195739996 hasRelatedWork W2063148885 @default.
• W195739996 hasRelatedWork W2063882402 @default.
• W195739996 hasRelatedWork W2065467435 @default.
• W195739996 hasRelatedWork W2086844275 @default.
• W195739996 hasRelatedWork W2086984521 @default.
• W195739996 hasRelatedWork W2106092051 @default.
• W195739996 hasRelatedWork W2127936483 @default.
• W195739996 hasRelatedWork W2131193695 @default.
• W195739996 hasRelatedWork W2131613604 @default.
• W195739996 hasRelatedWork W2150945637 @default.

• next