FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1957496223> ?p ?o ?g. }

• W1957496223 endingPage "442" @default.
• W1957496223 startingPage "433" @default.
• W1957496223 abstract "As photoautotrophic prokaryotes, cyanobacteria are promising platforms for producing value-added bioproducts. However, few regulatory genetic parts and devices (e.g., inducible promoters and regulatory circuits) have been developed for these potential hosts. Furthermore, the devices that have been created respond only to a single input. To address these issues, we developed an inducible genetic circuit that generates heterologous proteins in response to oxygen, an environmental signal. To test its performance and utility in Synechocystis sp. PCC 6803, a model cyanobacterial strain, we connected this circuit to either heterologous nifHDK genes, which encode oxygen-sensitive nitrogenase's structural proteins, or a fluorescent protein gene. The circuit was transcriptionally activated to generate nifHDK transcripts or fluorescent output only in low oxygen conditions. We expanded the oxygen-responsive circuit into a more complex circuit by building a two-input AND gate, which allows Synechocystis to specifically control expression of the fluorescent reporter in response to two signals, low oxygen and high anhydrotetracycline. To our knowledge, the AND gate is the first complex logic circuit built in a cyanobacterial strain. This work expands the synthetic biology tools available for complex gene expression in cyanobacteria, increasing their potential as biotechnology platforms." @default.
• W1957496223 created "2016-06-24" @default.
• W1957496223 creator A5023772503 @default.
• W1957496223 creator A5053005768 @default.
• W1957496223 creator A5058762818 @default.
• W1957496223 creator A5061963434 @default.
• W1957496223 creator A5069573028 @default.
• W1957496223 creator A5084701895 @default.
• W1957496223 date "2015-09-04" @default.
• W1957496223 modified "2023-09-27" @default.
• W1957496223 title "Oxygen‐responsive genetic circuits constructed in <i>Synechocystis</i> sp. PCC 6803" @default.
• W1957496223 cites W1020346317 @default.
• W1957496223 cites W1528872009 @default.
• W1957496223 cites W1543999230 @default.
• W1957496223 cites W1967024448 @default.
• W1957496223 cites W1969309283 @default.
• W1957496223 cites W1991123854 @default.
• W1957496223 cites W1991383594 @default.
• W1957496223 cites W1993506358 @default.
• W1957496223 cites W2002669907 @default.
• W1957496223 cites W2012010696 @default.
• W1957496223 cites W2015914231 @default.
• W1957496223 cites W2016097940 @default.
• W1957496223 cites W2016667239 @default.
• W1957496223 cites W2020044960 @default.
• W1957496223 cites W2021214021 @default.
• W1957496223 cites W2030695915 @default.
• W1957496223 cites W2038470803 @default.
• W1957496223 cites W2039278376 @default.
• W1957496223 cites W2045095831 @default.
• W1957496223 cites W2047360542 @default.
• W1957496223 cites W2049562870 @default.
• W1957496223 cites W2052593484 @default.
• W1957496223 cites W2053512440 @default.
• W1957496223 cites W2054522148 @default.
• W1957496223 cites W2057911693 @default.
• W1957496223 cites W2060964933 @default.
• W1957496223 cites W2068219766 @default.
• W1957496223 cites W2069820597 @default.
• W1957496223 cites W2083456183 @default.
• W1957496223 cites W2084470446 @default.
• W1957496223 cites W2087503372 @default.
• W1957496223 cites W2093574909 @default.
• W1957496223 cites W2098146748 @default.
• W1957496223 cites W2101406687 @default.
• W1957496223 cites W2105248706 @default.
• W1957496223 cites W2110927948 @default.
• W1957496223 cites W2111869546 @default.
• W1957496223 cites W2123467717 @default.
• W1957496223 cites W2127647062 @default.
• W1957496223 cites W2128556638 @default.
• W1957496223 cites W2132759650 @default.
• W1957496223 cites W2133248262 @default.
• W1957496223 cites W2136118838 @default.
• W1957496223 cites W2144876629 @default.
• W1957496223 cites W2148681712 @default.
• W1957496223 cites W2149711523 @default.
• W1957496223 cites W2150618302 @default.
• W1957496223 cites W2151104145 @default.
• W1957496223 cites W2162956402 @default.
• W1957496223 cites W2164957727 @default.
• W1957496223 cites W2165952036 @default.
• W1957496223 cites W2168420558 @default.
• W1957496223 cites W2170627796 @default.
• W1957496223 cites W316668102 @default.
• W1957496223 doi "https://doi.org/10.1002/bit.25722" @default.
• W1957496223 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26264210" @default.
• W1957496223 hasPublicationYear "2015" @default.
• W1957496223 type Work @default.
• W1957496223 sameAs 1957496223 @default.
• W1957496223 citedByCount "45" @default.
• W1957496223 countsByYear W19574962232016 @default.
• W1957496223 countsByYear W19574962232017 @default.
• W1957496223 countsByYear W19574962232018 @default.
• W1957496223 countsByYear W19574962232019 @default.
• W1957496223 countsByYear W19574962232020 @default.
• W1957496223 countsByYear W19574962232021 @default.
• W1957496223 countsByYear W19574962232022 @default.
• W1957496223 countsByYear W19574962232023 @default.
• W1957496223 crossrefType "journal-article" @default.
• W1957496223 hasAuthorship W1957496223A5023772503 @default.
• W1957496223 hasAuthorship W1957496223A5053005768 @default.
• W1957496223 hasAuthorship W1957496223A5058762818 @default.
• W1957496223 hasAuthorship W1957496223A5061963434 @default.
• W1957496223 hasAuthorship W1957496223A5069573028 @default.
• W1957496223 hasAuthorship W1957496223A5084701895 @default.
• W1957496223 hasConcept C101762097 @default.
• W1957496223 hasConcept C104317684 @default.
• W1957496223 hasConcept C141280058 @default.
• W1957496223 hasConcept C142613039 @default.
• W1957496223 hasConcept C150194340 @default.
• W1957496223 hasConcept C181440489 @default.
• W1957496223 hasConcept C191908910 @default.
• W1957496223 hasConcept C2779669040 @default.
• W1957496223 hasConcept C2780115692 @default.
• W1957496223 hasConcept C2780238584 @default.
• W1957496223 hasConcept C523546767 @default.
• W1957496223 hasConcept C54355233 @default.

• next