FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1957788286> ?p ?o ?g. }

• W1957788286 endingPage "145" @default.
• W1957788286 startingPage "139" @default.
• W1957788286 abstract "NADPH:protochlorophyllide oxidoreductase (POR) catalyses the light-dependent reduction of protochlorophyllide to chlorophyllide, a key reaction in the chlorophyll biosynthetic pathway. To facilitate structure-function studies, POR from pea (Pisum sativum L.) has been overexpressed in Escherichia coli as a fusion with maltose-binding protein (MBP) at 5-10% of the total soluble cell protein. The fusion protein (MBP-POR) has been purified to greater than 90% homogeneity by a two-step affinity-purification procedure. This represents the first successful overexpression and purification of a plant POR. MBP-POR was found to be active, and the kinetic properties were determined using a continuous assay in which the rate of chlorophyllide formation was measured. The Vmax was 20.6+/-0.9 nmol.min-1.mg-1 and the Km values for NADPH and protochlorophyllide were 8.7+/-1.9 microM and 0.27+/-0.04 microM respectively. These results represent the first determination of the kinetic properties of a pure POR and the first report on the kinetics of POR from a dicotyledenous plant. The experiments described here demonstrate that the enzyme is not a 'suicide' enzyme, and the only components required for catalysis are NADPH, protochlorophyllide and light. Size-exclusion chromatography on a Superose 6 HR column indicated that MBP-POR has a molecular mass of 155 kDa (compared with the molecular mass of 80 kDa estimated by SDS/PAGE), indicating that it behaves as a dimer in solution. This is the first direct determination of the oligomerization state of POR." @default.
• W1957788286 created "2016-06-24" @default.
• W1957788286 creator A5012429018 @default.
• W1957788286 creator A5058490241 @default.
• W1957788286 creator A5068638453 @default.
• W1957788286 date "1997-07-01" @default.
• W1957788286 modified "2023-09-25" @default.
• W1957788286 title "Purification and kinetic analysis of pea (Pisum sativum L.) NADPH:protochlorophyllide oxidoreductase expressed as a fusion with maltose-binding protein in Escherichia coli" @default.
• W1957788286 cites W104110300 @default.
• W1957788286 cites W1527191727 @default.
• W1957788286 cites W1547856877 @default.
• W1957788286 cites W161128883 @default.
• W1957788286 cites W1716042016 @default.
• W1957788286 cites W1793491524 @default.
• W1957788286 cites W1849836964 @default.
• W1957788286 cites W1860444002 @default.
• W1957788286 cites W2003067923 @default.
• W1957788286 cites W2013036004 @default.
• W1957788286 cites W2018289835 @default.
• W1957788286 cites W2025191038 @default.
• W1957788286 cites W2052619518 @default.
• W1957788286 cites W2065552173 @default.
• W1957788286 cites W2082497364 @default.
• W1957788286 cites W2084231554 @default.
• W1957788286 cites W2100837269 @default.
• W1957788286 cites W2117194968 @default.
• W1957788286 cites W2122307913 @default.
• W1957788286 cites W2127943529 @default.
• W1957788286 cites W2161642448 @default.
• W1957788286 cites W2169454844 @default.
• W1957788286 cites W2407450780 @default.
• W1957788286 cites W75650367 @default.
• W1957788286 doi "https://doi.org/10.1042/bj3250139" @default.
• W1957788286 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1218538" @default.
• W1957788286 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9224639" @default.
• W1957788286 hasPublicationYear "1997" @default.
• W1957788286 type Work @default.
• W1957788286 sameAs 1957788286 @default.
• W1957788286 citedByCount "59" @default.
• W1957788286 countsByYear W19577882862012 @default.
• W1957788286 countsByYear W19577882862014 @default.
• W1957788286 countsByYear W19577882862015 @default.
• W1957788286 countsByYear W19577882862016 @default.
• W1957788286 countsByYear W19577882862019 @default.
• W1957788286 countsByYear W19577882862020 @default.
• W1957788286 countsByYear W19577882862021 @default.
• W1957788286 countsByYear W19577882862022 @default.
• W1957788286 crossrefType "journal-article" @default.
• W1957788286 hasAuthorship W1957788286A5012429018 @default.
• W1957788286 hasAuthorship W1957788286A5058490241 @default.
• W1957788286 hasAuthorship W1957788286A5068638453 @default.
• W1957788286 hasBestOaLocation W19577882862 @default.
• W1957788286 hasConcept C104317684 @default.
• W1957788286 hasConcept C123894998 @default.
• W1957788286 hasConcept C178790620 @default.
• W1957788286 hasConcept C179247698 @default.
• W1957788286 hasConcept C181199279 @default.
• W1957788286 hasConcept C185592680 @default.
• W1957788286 hasConcept C2779268744 @default.
• W1957788286 hasConcept C2779520027 @default.
• W1957788286 hasConcept C2779535332 @default.
• W1957788286 hasConcept C2779671804 @default.
• W1957788286 hasConcept C2780676925 @default.
• W1957788286 hasConcept C2781323092 @default.
• W1957788286 hasConcept C40767141 @default.
• W1957788286 hasConcept C41183919 @default.
• W1957788286 hasConcept C547475151 @default.
• W1957788286 hasConcept C55493867 @default.
• W1957788286 hasConcept C56856141 @default.
• W1957788286 hasConcept C86803240 @default.
• W1957788286 hasConceptScore W1957788286C104317684 @default.
• W1957788286 hasConceptScore W1957788286C123894998 @default.
• W1957788286 hasConceptScore W1957788286C178790620 @default.
• W1957788286 hasConceptScore W1957788286C179247698 @default.
• W1957788286 hasConceptScore W1957788286C181199279 @default.
• W1957788286 hasConceptScore W1957788286C185592680 @default.
• W1957788286 hasConceptScore W1957788286C2779268744 @default.
• W1957788286 hasConceptScore W1957788286C2779520027 @default.
• W1957788286 hasConceptScore W1957788286C2779535332 @default.
• W1957788286 hasConceptScore W1957788286C2779671804 @default.
• W1957788286 hasConceptScore W1957788286C2780676925 @default.
• W1957788286 hasConceptScore W1957788286C2781323092 @default.
• W1957788286 hasConceptScore W1957788286C40767141 @default.
• W1957788286 hasConceptScore W1957788286C41183919 @default.
• W1957788286 hasConceptScore W1957788286C547475151 @default.
• W1957788286 hasConceptScore W1957788286C55493867 @default.
• W1957788286 hasConceptScore W1957788286C56856141 @default.
• W1957788286 hasConceptScore W1957788286C86803240 @default.
• W1957788286 hasIssue "1" @default.
• W1957788286 hasLocation W19577882861 @default.
• W1957788286 hasLocation W19577882862 @default.
• W1957788286 hasLocation W19577882863 @default.
• W1957788286 hasLocation W19577882864 @default.
• W1957788286 hasOpenAccess W1957788286 @default.
• W1957788286 hasPrimaryLocation W19577882861 @default.
• W1957788286 hasRelatedWork W181294179 @default.
• W1957788286 hasRelatedWork W1957788286 @default.
• W1957788286 hasRelatedWork W1981819223 @default.

• next