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• W1958333654 startingPage "1511" @default.
• W1958333654 abstract "N 6 -methyladenosine (m 6 A), the most abundant internal RNA modification, functions in diverse biological processes, including regulation of embryonic stem cell self-renewal and differentiation. As yet, methods to detect m 6 A in the transcriptome rely on the availability and quality of an m 6 A antibody and are often associated with a high rate of false positives. Here, based on our observation that m 6 A interferes with A–T/U pairing, we report a microarray-based technology to map m 6 A sites in mouse embryonic stem cells. We identified 72 unbiased sites exhibiting high m 6 A levels from 66 PolyA RNAs. Bioinformatics analyses suggest identified sites are enriched on developmental regulators and may in some contexts modulate microRNA/mRNA interactions. Overall, we have developed microarray-based technology to capture highly enriched m 6 A sites in the mammalian transcriptome. This method provides an alternative means to identify m 6 A sites for certain applications." @default.
• W1958333654 created "2016-06-24" @default.
• W1958333654 creator A5009847434 @default.
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• W1958333654 date "2015-06-19" @default.
• W1958333654 modified "2023-10-14" @default.
• W1958333654 title "Genome-wide detection of high abundance <i>N</i>⁶-methyladenosine sites by microarray" @default.
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• W1958333654 doi "https://doi.org/10.1261/rna.051474.115" @default.
• W1958333654 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4509940" @default.
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• W1958333654 hasPublicationYear "2015" @default.
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