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- W1958388479 abstract "Summary Recent methylome analyses of the entire Arabidopsis thaliana genome using various mutants have provided detailed information about the DNA methylation pattern and its function. However, information about DNA methylation in other plants is limited, partly because of the lack of mutants. To study DNA methylation in rice ( Oryza sativa ) we applied homologous recombination‐mediated gene targeting to generate targeted disruptants of OsDRM2 , a rice orthologue of DOMAINS REARRANGED METHYLASE 1 and 2 ( DRM1/2 ), which encode DNA methyltransferases responsible for de novo and non‐CG methylation in Arabidopsis. Whereas Arabidopsis drm1 drm2 double mutants showed no morphological alterations, targeted disruptants of rice OsDRM2 displayed pleiotropic developmental phenotypes in both vegetative and reproductive stages, including growth defects, semi‐dwarfed stature, reductions in tiller number, delayed heading or no heading, abnormal panicle and spikelet morphology, and complete sterility. In these osdrm2 disruptants, a 13.9% decrease in 5‐methylcytosine was observed by HPLC analysis. The CG and non‐CG methylation levels were reduced in RIRE7/CRR1 retrotransposons, and in 5S rDNA repeats. Associated transcriptional activation was detected in RIRE7/CRR1. Furthermore, de novo methylation by an RNA‐directed DNA methylation (RdDM) process involving transgene‐derived exogenous small interfering RNA (siRNA) was deficient in osdrm2 ‐disrupted cells. Impaired growth and abnormal DNA methylation of osdrm2 disruptants were restored by the complementation of wild‐type OsDRM2 cDNA. Our results suggest that OsDRM2 is responsible for de novo , CG and non‐CG methylation in rice genomic sequences, and that DNA methylation regulated by OsDRM2 is essential for proper rice development in both vegetative and reproductive stages." @default.
- W1958388479 created "2016-06-24" @default.
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- W1958388479 date "2012-04-26" @default.
- W1958388479 modified "2023-10-16" @default.
- W1958388479 title "Targeted disruption of an orthologue of DOMAINS REARRANGED METHYLASE 2, OsDRM2, impairs the growth of rice plants by abnormal DNA methylation" @default.
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- W1958388479 doi "https://doi.org/10.1111/j.1365-313x.2012.04974.x" @default.
- W1958388479 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22380881" @default.
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