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- W1958596549 abstract "Biologically-active β-peptides and pharmaceuticals that contain key β-amino acids are emerging as target therapeutics; thus, synthetic strategies to make substituted, enantiopure β-amino acids are increasing. Here, we use whole-cell Escherichia coli (OD600 ∼35) engineered to express a Pantoea agglomerans phenylalanine aminomutase (PaPAM) biocatalyst. In either 5 mL, 100 mL, or 1 L of M9 minimal medium containing α-phenylalanine (20 mM), the cells produced ∼1.4 mg mL−1 of β-phenylalanine in each volume. Representative pilot-scale 5-mL cultures, fermentation reactions converted 18 variously substituted α-arylalanines to their (S)-β-aryl-β-amino acids in vivo and were not toxic to cells at mid- to late-stage growth. The β-aryl-β-amino acids made ranged from 0.043 mg (p-nitro-β-phenylalanine, 4% converted yield) to 1.2 mg (m-bromo-β-phenylalanine, 96% converted yield) over 6 h in 5 mL. The substituted β-amino acids made herein can be used in redox and Stille-coupling reactions to make synthetic building blocks, or as bioisosteres in drug design." @default.
- W1958596549 created "2016-06-24" @default.
- W1958596549 creator A5006888491 @default.
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- W1958596549 date "2016-01-01" @default.
- W1958596549 modified "2023-10-16" @default.
- W1958596549 title "Whole-cell biocatalytic production of variously substituted β-aryl- and β-heteroaryl-β-amino acids" @default.
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- W1958596549 doi "https://doi.org/10.1016/j.jbiotec.2015.10.012" @default.
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