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- W195930969 abstract "Phenotypic identification of pre-Bötzinger complex (pre-BötC) neurons is vital to the delineation of endogenous cellular properties involved in respiratory rhythm generation and neuromodulation of breathing. A subpopulation of pre-BötC neurons endowed with intrinsic rhythmicity is proposed as the kernel for inspiratory rhythm generation in vitro. In this study, the pre-BötC island preparation was excised from medullary slices and reduced to its heterogeneous cellular constituents using primary cell culture techniques. Cultured neurons were labeled with tetramethylrhodamine conjugated to Substance P (TMR-SP) to identify individual NK-1R expressing neurons. Data from this study provide initial evidence that the pre-BötC neurons living in culture remain functionally capable of binding and internalizing the fluorescently-tagged ligand and TMR-SP tagging is a useful experimental tool that reliably identifies the NK-1R phenotype." @default.
- W195930969 created "2016-06-24" @default.
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- W195930969 date "2008-01-01" @default.
- W195930969 modified "2023-09-26" @default.
- W195930969 title "Pre-Bötzinger Complex Neurokinin-1 Receptor Expressing Neurons in Primary Cell Culture" @default.
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- W195930969 doi "https://doi.org/10.1007/978-0-387-73693-8_16" @default.
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