FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1959660762> ?p ?o ?g. }

• W1959660762 abstract "PARP inhibitors (PARPi) are a novel class of drugs with activity in patients with acquired or germline homologous recombination (HR) deficiency-associated high-grade serous ovarian cancer (HGSOC). We hypothesized that measuring γH2AX as an indicator of DNA double-strand breaks (DSB), and MRE11 or RAD51 as an indicator of DSB repair, would reflect HR status and predict response to PARPi-based therapy. Our aim was to develop and use high-throughput multiparametric flow cytometry to quantify γH2AX with MRE11 or RAD51 in PBMCs as a readily available surrogate.Healthy donor PBMCs were used for assay development and optimization. We validated induction of γH2AX, MRE11 and RAD51 by staining with fluorophore-conjugated antibodies. The multiparameter flow cytometric method was applied to PBMC samples from recurrent HGSOC patients who were treated with PARPi, olaparib and carboplatin.Stimulation was necessary for quantification of a DNA damage response to olaparib/carboplatin in healthy donor PBMCs. The flow cytometric protocol could not distinguish between cytoplasmic and nuclear RAD51, erroneously indicating activation in response to injury. Thus, MRE11 was selected as the marker of DSB repair. PBMCs from 15 recurrent HGSOC patients were then examined. Patients who did not respond to PARPi therapy had a significantly higher pre-treatment level of γH2AX (p = 0.01), and a higher ratio of γH2AX/MRE11 (11.0 [3.5-13.2] v. 3.3 [2.8-9.9], p < 0.03) compared with responders.We successfully developed and applied a multiparameter flow cytometry assay to measure γH2AX and MRE11 in PBMCs. Prospective studies will be required to validate this surrogate biomarker assay as a potential predictive biomarker of PARPi-based therapy." @default.
• W1959660762 created "2016-06-24" @default.
• W1959660762 creator A5005621340 @default.
• W1959660762 creator A5008300019 @default.
• W1959660762 creator A5024336775 @default.
• W1959660762 creator A5040289016 @default.
• W1959660762 creator A5057129475 @default.
• W1959660762 creator A5059967384 @default.
• W1959660762 date "2015-07-22" @default.
• W1959660762 modified "2023-10-07" @default.
• W1959660762 title "Development of a multiparameter flow cytometric assay as a potential biomarker for homologous recombination deficiency in women with high-grade serous ovarian cancer" @default.
• W1959660762 cites W1638124759 @default.
• W1959660762 cites W1963673805 @default.
• W1959660762 cites W1969839636 @default.
• W1959660762 cites W1978510751 @default.
• W1959660762 cites W1979269833 @default.
• W1959660762 cites W1981961534 @default.
• W1959660762 cites W1987335009 @default.
• W1959660762 cites W1989362916 @default.
• W1959660762 cites W2007865556 @default.
• W1959660762 cites W2012917573 @default.
• W1959660762 cites W2017275209 @default.
• W1959660762 cites W2019405732 @default.
• W1959660762 cites W2021147271 @default.
• W1959660762 cites W2027375553 @default.
• W1959660762 cites W2027630134 @default.
• W1959660762 cites W2030256991 @default.
• W1959660762 cites W2037694031 @default.
• W1959660762 cites W2044271955 @default.
• W1959660762 cites W2058812851 @default.
• W1959660762 cites W2059326356 @default.
• W1959660762 cites W2060436524 @default.
• W1959660762 cites W2064377499 @default.
• W1959660762 cites W2068769666 @default.
• W1959660762 cites W2074064462 @default.
• W1959660762 cites W2077442319 @default.
• W1959660762 cites W2081260575 @default.
• W1959660762 cites W2093463836 @default.
• W1959660762 cites W2096530939 @default.
• W1959660762 cites W2106951948 @default.
• W1959660762 cites W2107208323 @default.
• W1959660762 cites W2109001339 @default.
• W1959660762 cites W2114280096 @default.
• W1959660762 cites W2114572046 @default.
• W1959660762 cites W2117639046 @default.
• W1959660762 cites W2119186872 @default.
• W1959660762 cites W2123696077 @default.
• W1959660762 cites W2130078242 @default.
• W1959660762 cites W2137432910 @default.
• W1959660762 cites W2139511888 @default.
• W1959660762 cites W2144277176 @default.
• W1959660762 cites W2145200068 @default.
• W1959660762 cites W2145623483 @default.
• W1959660762 cites W2145676287 @default.
• W1959660762 cites W2147111123 @default.
• W1959660762 cites W2147476574 @default.
• W1959660762 cites W2157962151 @default.
• W1959660762 cites W2161405893 @default.
• W1959660762 cites W2170804283 @default.
• W1959660762 cites W2170926194 @default.
• W1959660762 doi "https://doi.org/10.1186/s12967-015-0604-z" @default.
• W1959660762 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4508767" @default.
• W1959660762 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26198537" @default.
• W1959660762 hasPublicationYear "2015" @default.
• W1959660762 type Work @default.
• W1959660762 sameAs 1959660762 @default.
• W1959660762 citedByCount "15" @default.
• W1959660762 countsByYear W19596607622016 @default.
• W1959660762 countsByYear W19596607622017 @default.
• W1959660762 countsByYear W19596607622018 @default.
• W1959660762 countsByYear W19596607622019 @default.
• W1959660762 countsByYear W19596607622020 @default.
• W1959660762 countsByYear W19596607622021 @default.
• W1959660762 countsByYear W19596607622022 @default.
• W1959660762 crossrefType "journal-article" @default.
• W1959660762 hasAuthorship W1959660762A5005621340 @default.
• W1959660762 hasAuthorship W1959660762A5008300019 @default.
• W1959660762 hasAuthorship W1959660762A5024336775 @default.
• W1959660762 hasAuthorship W1959660762A5040289016 @default.
• W1959660762 hasAuthorship W1959660762A5057129475 @default.
• W1959660762 hasAuthorship W1959660762A5059967384 @default.
• W1959660762 hasBestOaLocation W19596607621 @default.
• W1959660762 hasConcept C102744134 @default.
• W1959660762 hasConcept C126322002 @default.
• W1959660762 hasConcept C134935766 @default.
• W1959660762 hasConcept C137061746 @default.
• W1959660762 hasConcept C143425029 @default.
• W1959660762 hasConcept C143998085 @default.
• W1959660762 hasConcept C153911025 @default.
• W1959660762 hasConcept C182979987 @default.
• W1959660762 hasConcept C202751555 @default.
• W1959660762 hasConcept C203014093 @default.
• W1959660762 hasConcept C2776694085 @default.
• W1959660762 hasConcept C2778239845 @default.
• W1959660762 hasConcept C2779012473 @default.
• W1959660762 hasConcept C2779138821 @default.
• W1959660762 hasConcept C2779962180 @default.
• W1959660762 hasConcept C2781451048 @default.
• W1959660762 hasConcept C502942594 @default.
• W1959660762 hasConcept C54355233 @default.

• next