FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1960740641> ?p ?o ?g. }

• W1960740641 endingPage "1145" @default.
• W1960740641 startingPage "1135" @default.
• W1960740641 abstract "A 600-bp oriT-containing DNA fragment from the Rhodobacter sphaeroides 2.4.1 S factor (oriTs) (A. Suwanto and S. Kaplan, J. Bacteriol. 174:1124-1134, 1992) was shown to promote polarized chromosomal transfer when provided in cis. A Kmr-oriTs-sacR-sacB (KTS) DNA cassette was constructed by inserting oriTs-sacR-sacB into a pUTmini-Tn5 Km1 derivative. With this delivery system, KTS appeared to be randomly inserted into the genome of R. sphaeroides, generating mutant strains which also gained the ability to act as Hfr donors. An AseI site in the Kmr cartridge (from Tn903) and DraI and SnaBI sites in sacR-sacB (the levansucrase gene from Bacillus subtilis) were employed to localize the KTS insertion definitively by pulsed-field gel electrophoresis. The orientation of oriTs at the site of insertion was determined by Southern hybridization analysis. Interrupted mating experiments performed with some of the Hfr strains exhibited a gradient of marker transfer and further provided genetic evidence for the circularity and presence of two chromosomal linkage groups in this bacterium. The genetic and environmental conditions for optimized mating between R. sphaeroides strains were also defined. The results presented here and our physical map of the R. sphaeroides 2.4.1 genome are discussed in light of the presence of two chromosomes." @default.
• W1960740641 created "2016-06-24" @default.
• W1960740641 creator A5020456363 @default.
• W1960740641 creator A5075539611 @default.
• W1960740641 date "1992-02-01" @default.
• W1960740641 modified "2023-09-26" @default.
• W1960740641 title "Chromosome transfer in Rhodobacter sphaeroides: Hfr formation and genetic evidence for two unique circular chromosomes" @default.
• W1960740641 cites W1493961769 @default.
• W1960740641 cites W1497026696 @default.
• W1960740641 cites W1513441571 @default.
• W1960740641 cites W1518825157 @default.
• W1960740641 cites W1548594428 @default.
• W1960740641 cites W1607350177 @default.
• W1960740641 cites W1636002209 @default.
• W1960740641 cites W1694199808 @default.
• W1960740641 cites W1742959348 @default.
• W1960740641 cites W1745792303 @default.
• W1960740641 cites W1819455144 @default.
• W1960740641 cites W1824614244 @default.
• W1960740641 cites W1852145520 @default.
• W1960740641 cites W1905789791 @default.
• W1960740641 cites W1916826035 @default.
• W1960740641 cites W1952076072 @default.
• W1960740641 cites W1965334884 @default.
• W1960740641 cites W1967164846 @default.
• W1960740641 cites W1987355768 @default.
• W1960740641 cites W1988711630 @default.
• W1960740641 cites W2027229485 @default.
• W1960740641 cites W2028796767 @default.
• W1960740641 cites W2037751240 @default.
• W1960740641 cites W2045712913 @default.
• W1960740641 cites W2050925099 @default.
• W1960740641 cites W2054805561 @default.
• W1960740641 cites W2064539276 @default.
• W1960740641 cites W2069595322 @default.
• W1960740641 cites W2071193620 @default.
• W1960740641 cites W2090282828 @default.
• W1960740641 cites W2093072845 @default.
• W1960740641 cites W2101488790 @default.
• W1960740641 cites W2102407918 @default.
• W1960740641 cites W2112588210 @default.
• W1960740641 cites W2126102570 @default.
• W1960740641 cites W2161524167 @default.
• W1960740641 cites W2169921363 @default.
• W1960740641 cites W2219114844 @default.
• W1960740641 cites W2896859644 @default.
• W1960740641 cites W4211022623 @default.
• W1960740641 doi "https://doi.org/10.1128/jb.174.4.1135-1145.1992" @default.
• W1960740641 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/206406" @default.
• W1960740641 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1735708" @default.
• W1960740641 hasPublicationYear "1992" @default.
• W1960740641 type Work @default.
• W1960740641 sameAs 1960740641 @default.
• W1960740641 citedByCount "63" @default.
• W1960740641 countsByYear W19607406412012 @default.
• W1960740641 countsByYear W19607406412014 @default.
• W1960740641 countsByYear W19607406412022 @default.
• W1960740641 crossrefType "journal-article" @default.
• W1960740641 hasAuthorship W1960740641A5020456363 @default.
• W1960740641 hasAuthorship W1960740641A5075539611 @default.
• W1960740641 hasBestOaLocation W19607406412 @default.
• W1960740641 hasConcept C104317684 @default.
• W1960740641 hasConcept C109297637 @default.
• W1960740641 hasConcept C141231307 @default.
• W1960740641 hasConcept C143065580 @default.
• W1960740641 hasConcept C2777850835 @default.
• W1960740641 hasConcept C2777897970 @default.
• W1960740641 hasConcept C30481170 @default.
• W1960740641 hasConcept C523546767 @default.
• W1960740641 hasConcept C54355233 @default.
• W1960740641 hasConcept C547475151 @default.
• W1960740641 hasConcept C86803240 @default.
• W1960740641 hasConceptScore W1960740641C104317684 @default.
• W1960740641 hasConceptScore W1960740641C109297637 @default.
• W1960740641 hasConceptScore W1960740641C141231307 @default.
• W1960740641 hasConceptScore W1960740641C143065580 @default.
• W1960740641 hasConceptScore W1960740641C2777850835 @default.
• W1960740641 hasConceptScore W1960740641C2777897970 @default.
• W1960740641 hasConceptScore W1960740641C30481170 @default.
• W1960740641 hasConceptScore W1960740641C523546767 @default.
• W1960740641 hasConceptScore W1960740641C54355233 @default.
• W1960740641 hasConceptScore W1960740641C547475151 @default.
• W1960740641 hasConceptScore W1960740641C86803240 @default.
• W1960740641 hasIssue "4" @default.
• W1960740641 hasLocation W19607406411 @default.
• W1960740641 hasLocation W19607406412 @default.
• W1960740641 hasLocation W19607406413 @default.
• W1960740641 hasLocation W19607406414 @default.
• W1960740641 hasOpenAccess W1960740641 @default.
• W1960740641 hasPrimaryLocation W19607406411 @default.
• W1960740641 hasRelatedWork W1550062366 @default.
• W1960740641 hasRelatedWork W2031436818 @default.
• W1960740641 hasRelatedWork W2047294529 @default.
• W1960740641 hasRelatedWork W2069184423 @default.
• W1960740641 hasRelatedWork W2117110650 @default.
• W1960740641 hasRelatedWork W2122720419 @default.
• W1960740641 hasRelatedWork W2125841156 @default.
• W1960740641 hasRelatedWork W2137635963 @default.

• next