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• W1961656476 abstract "Post-transcriptional methylation of RNA cytosine residues to 5-methylcytosine (m(5)C) is an important modification that regulates RNA metabolism and occurs in both eukaryotes and prokaryotes. Yet, to date, no transcriptome-wide identification of m(5)C sites has been undertaken in plants. Plants provide a unique comparative system for investigating the origin and evolution of m(5)C as they contain three different genomes, the nucleus, mitochondria and chloroplast. Here we use bisulfite conversion of RNA combined with high-throughput IIlumina sequencing (RBS-seq) to identify single-nucleotide resolution of m(5)C sites in non-coding ribosomal RNAs and transfer RNAs of all three sub-cellular transcriptomes across six diverse species that included, the single-celled algae Nannochloropsis oculata, the macro algae Caulerpa taxifolia and multi-cellular higher plants Arabidopsis thaliana, Brassica rapa, Triticum durum and Ginkgo biloba.Using the plant model Arabidopsis thaliana, we identified a total of 39 highly methylated m(5)C sites in predicted structural positions of nuclear tRNAs and 7 m(5)C sites in rRNAs from nuclear, chloroplast and mitochondrial transcriptomes. Both the nucleotide position and percent methylation of tRNAs and rRNAs m(5)C sites were conserved across all species analysed, from single celled algae N. oculata to multicellular plants. Interestingly the mitochondrial and chloroplast encoded tRNAs were devoid of m(5)C in A. thaliana and this is generally conserved across Plantae. This suggests independent evolution of organelle methylation in animals and plants, as animal mitochondrial tRNAs have m(5)C sites. Here we characterize 5 members of the RNA 5-methylcytosine family in Arabidopsis and extend the functional characterization of TRDMT1 and NOP2A/OLI2. We demonstrate that nuclear tRNA methylation requires two evolutionarily conserved methyltransferases, TRDMT1 and TRM4B. trdmt1 trm4b double mutants are hypersensitive to the antibiotic hygromycin B, demonstrating the function of tRNA methylation in regulating translation. Additionally we demonstrate that nuclear large subunit 25S rRNA methylation requires the conserved RNA methyltransferase NSUN5. Our results also suggest functional redundancy of at least two of the NOP2 paralogs in Arabidopsis.Our data demonstrates widespread occurrence and conservation of non-coding RNA methylation in the kingdom Plantae, suggesting important and highly conserved roles of this post-transcriptional modification." @default.
• W1961656476 created "2016-06-24" @default.
• W1961656476 creator A5045940475 @default.
• W1961656476 creator A5065873112 @default.
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• W1961656476 date "2015-08-14" @default.
• W1961656476 modified "2023-10-07" @default.
• W1961656476 title "Conservation of tRNA and rRNA 5-methylcytosine in the kingdom Plantae" @default.
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• W1961656476 cites W1984451904 @default.
• W1961656476 cites W1990536696 @default.
• W1961656476 cites W1992773451 @default.
• W1961656476 cites W2005311687 @default.
• W1961656476 cites W2006710298 @default.
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• W1961656476 cites W2028018746 @default.
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• W1961656476 cites W2044954993 @default.
• W1961656476 cites W2045352663 @default.
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• W1961656476 doi "https://doi.org/10.1186/s12870-015-0580-8" @default.
• W1961656476 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4535395" @default.
• W1961656476 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26268215" @default.
• W1961656476 hasPublicationYear "2015" @default.
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