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- W1962144974 abstract "Abstract The cosQ site of bacteriophage λ is required for DNA packaging termination. Previous studies have shown that cosQ mutations can be suppressed in three ways: by a local suppressor within cosQ, an increase in the length of the λ chromosome, and missense mutations affecting the prohead’s portal protein, gpB. In the present work, revertants of a set of lethal cosQ mutants were screened for suppressors. Seven new cosQ suppressors affected gene B, which encodes the portal protein of the prohead. All seven were allelenonspecific suppressors of cosQ mutations. Experiments with several phages having two cosQ suppressors showed that the suppression effects were additive. Furthermore, these double suppressors had minimal effects on the growth of cosQ+ phages. These trans-acting suppressors affecting the portal protein are proposed to allow the mutant cosQ site to be more efficiently recognized, due to the slowing of the rate of translocation." @default.
- W1962144974 created "2016-06-24" @default.
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- W1962144974 creator A5076483391 @default.
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- W1962144974 date "2002-05-01" @default.
- W1962144974 modified "2023-09-26" @default.
- W1962144974 title "Alterations of the Portal Protein, gpB, of Bacteriophage λ Suppress Mutations in <i>cosQ</i>, the Site Required for Termination of DNA Packaging" @default.
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- W1962144974 doi "https://doi.org/10.1093/genetics/161.1.21" @default.
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