FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1962991321> ?p ?o ?g. }

• W1962991321 endingPage "1855" @default.
• W1962991321 startingPage "1849" @default.
• W1962991321 abstract "Complement plays a key role in phagocyte recognition and killing of Blastomyces dermatitidis, but little is known about how complement components interact with the yeast. We report the characteristics of activation, binding, and processing of C3 by B. dermatitidis. In pooled normal human serum (NHS), deposition of C3 on the yeast was detectable within 2 min, whereas in NHS containing MgEGTA, deposition was delayed by 6 min, indicating that the yeast activates C3 by both classical and alternative pathways. When both pathways were operative, maximal binding of 4.5 x 10(6) C3 molecules/cell was achieved in less than 30 min. In the absence of the classical pathway, yeast cells bound 80% of the maximum C3, indicating that the yeast intrinsically activates the alternative pathway. Delayed deposition of C3 in NHS-MgEGTA was similar to that in NHS preabsorbed by the yeast or by immobilized protein A/G to remove serum immunoglobulin. Purified immunoglobulin restored C3 binding to NHS preabsorbed by the yeast, suggesting that antibody in nonimmune serum initiates the classical pathway. beta-Glucan absorption of NHS abolished the classical pathway, suggesting that cell wall beta-glucan is a target of initiating antibodies. Hydroxylamine treatment of NHS-opsonized yeast cells showed that 76% of C3 was bound to yeast cells by ester linkage, supporting a role for hydroxyl groups on cell wall polysaccharides. Hydroxylamine-cleaved fragments were chiefly C3b and iC3b; 70% of hydroxylamine-sensitive C3b was converted to iC3b within 1 min of opsonization, and the ratio was stable over 1 h. Our data predict that C3b and iC3b on opsonized yeast cells direct binding to CR1 and CR3 receptors on human phagocytes, which, in turn, may influence the fate of this host-pathogen interaction." @default.
• W1962991321 created "2016-06-24" @default.
• W1962991321 creator A5010768212 @default.
• W1962991321 creator A5056626048 @default.
• W1962991321 date "1997-05-01" @default.
• W1962991321 modified "2023-09-24" @default.
• W1962991321 title "Activation, binding, and processing of complement component 3 (C3) by Blastomyces dermatitidis" @default.
• W1962991321 cites W112826064 @default.
• W1962991321 cites W1498261686 @default.
• W1962991321 cites W1522572101 @default.
• W1962991321 cites W1524025995 @default.
• W1962991321 cites W1527643370 @default.
• W1962991321 cites W1528847106 @default.
• W1962991321 cites W1561646576 @default.
• W1962991321 cites W1780395944 @default.
• W1962991321 cites W1783226291 @default.
• W1962991321 cites W1892422202 @default.
• W1962991321 cites W1915040721 @default.
• W1962991321 cites W1945415624 @default.
• W1962991321 cites W1946723375 @default.
• W1962991321 cites W1979379453 @default.
• W1962991321 cites W1987216284 @default.
• W1962991321 cites W2017520388 @default.
• W1962991321 cites W2020469064 @default.
• W1962991321 cites W203146259 @default.
• W1962991321 cites W2039677737 @default.
• W1962991321 cites W2097521148 @default.
• W1962991321 cites W2125033177 @default.
• W1962991321 cites W2126216740 @default.
• W1962991321 cites W2129047172 @default.
• W1962991321 cites W2143988227 @default.
• W1962991321 cites W2153309282 @default.
• W1962991321 cites W2170411697 @default.
• W1962991321 cites W2205167540 @default.
• W1962991321 cites W1486232015 @default.
• W1962991321 doi "https://doi.org/10.1128/iai.65.5.1849-1855.1997" @default.
• W1962991321 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/175230" @default.
• W1962991321 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9125571" @default.
• W1962991321 hasPublicationYear "1997" @default.
• W1962991321 type Work @default.
• W1962991321 sameAs 1962991321 @default.
• W1962991321 citedByCount "26" @default.
• W1962991321 countsByYear W19629913212013 @default.
• W1962991321 countsByYear W19629913212014 @default.
• W1962991321 countsByYear W19629913212018 @default.
• W1962991321 countsByYear W19629913212020 @default.
• W1962991321 countsByYear W19629913212021 @default.
• W1962991321 countsByYear W19629913212022 @default.
• W1962991321 crossrefType "journal-article" @default.
• W1962991321 hasAuthorship W1962991321A5010768212 @default.
• W1962991321 hasAuthorship W1962991321A5056626048 @default.
• W1962991321 hasBestOaLocation W19629913211 @default.
• W1962991321 hasConcept C111684460 @default.
• W1962991321 hasConcept C153911025 @default.
• W1962991321 hasConcept C159654299 @default.
• W1962991321 hasConcept C168507396 @default.
• W1962991321 hasConcept C171279029 @default.
• W1962991321 hasConcept C189446657 @default.
• W1962991321 hasConcept C191590540 @default.
• W1962991321 hasConcept C202751555 @default.
• W1962991321 hasConcept C203014093 @default.
• W1962991321 hasConcept C2777576037 @default.
• W1962991321 hasConcept C2779222958 @default.
• W1962991321 hasConcept C2779299957 @default.
• W1962991321 hasConcept C2779602430 @default.
• W1962991321 hasConcept C55493867 @default.
• W1962991321 hasConcept C86803240 @default.
• W1962991321 hasConcept C89372155 @default.
• W1962991321 hasConcept C89423630 @default.
• W1962991321 hasConceptScore W1962991321C111684460 @default.
• W1962991321 hasConceptScore W1962991321C153911025 @default.
• W1962991321 hasConceptScore W1962991321C159654299 @default.
• W1962991321 hasConceptScore W1962991321C168507396 @default.
• W1962991321 hasConceptScore W1962991321C171279029 @default.
• W1962991321 hasConceptScore W1962991321C189446657 @default.
• W1962991321 hasConceptScore W1962991321C191590540 @default.
• W1962991321 hasConceptScore W1962991321C202751555 @default.
• W1962991321 hasConceptScore W1962991321C203014093 @default.
• W1962991321 hasConceptScore W1962991321C2777576037 @default.
• W1962991321 hasConceptScore W1962991321C2779222958 @default.
• W1962991321 hasConceptScore W1962991321C2779299957 @default.
• W1962991321 hasConceptScore W1962991321C2779602430 @default.
• W1962991321 hasConceptScore W1962991321C55493867 @default.
• W1962991321 hasConceptScore W1962991321C86803240 @default.
• W1962991321 hasConceptScore W1962991321C89372155 @default.
• W1962991321 hasConceptScore W1962991321C89423630 @default.
• W1962991321 hasIssue "5" @default.
• W1962991321 hasLocation W19629913211 @default.
• W1962991321 hasLocation W19629913212 @default.
• W1962991321 hasLocation W19629913213 @default.
• W1962991321 hasOpenAccess W1962991321 @default.
• W1962991321 hasPrimaryLocation W19629913211 @default.
• W1962991321 hasRelatedWork W1562223438 @default.
• W1962991321 hasRelatedWork W1572830361 @default.
• W1962991321 hasRelatedWork W1962991321 @default.
• W1962991321 hasRelatedWork W1968740939 @default.
• W1962991321 hasRelatedWork W2061388543 @default.
• W1962991321 hasRelatedWork W2086671273 @default.

• next