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- W1963036077 abstract "We tested the utility of a 230 base pair intron fragment of the highly conserved nuclear gene Elongation Factor 1-alpha (EF1-α) as a proper marker to reconstruct the phylogeography of the marine amphipod Pontogammarus maeoticus (Sowinsky, 1894) from the Caspian and Black Seas. As a prerequisite for further analysis, we confirmed by Southern blot analysis that EF1-α is encoded at a single locus in P. maeoticus. We included 15 populations and 60 individuals in the study. Both the phylogeny of the 27 unique alleles found and population genetic analyses revealed a significant differentiation between populations from the aforementioned sea basins. Our results are in remarkable agreement with recent studies on a variety of species from the same area, which invariably support a major phylogeographic break between the Caspian and Black Seas. We thus conclude that our EF1-α intron is an informative marker for phylogeographic studies in amphipods at the shallow population level." @default.
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- W1963036077 date "2012-01-01" @default.
- W1963036077 modified "2023-09-25" @default.
- W1963036077 title "Intron structure of the Elongation Factor 1-Alpha gene in the Ponto-Caspian amphipod Pontogammarus maeoticus (Sowinsky, 1894) and its phylogeographic utility" @default.
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- W1963036077 doi "https://doi.org/10.1163/193724012x626584" @default.
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