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- W1963483977 abstract "The localization of acid mucopolysaccharide in tissues was studied by both the ruthenium red method by Luft and its modified method by Handley, and the penetration of ruthenium red into tissues investigated by above two methods was compared. Fetal mouse calvaria were cultured in plastic dishes containing α-MEM supplemented with 10% newborn bovine serum for about 10 days and submitted to the electron microscope. When the cultured tissue was fixed with ruthenium red-OsO4 solution which was designated as a postfixative prepared 3 hours prior to use, according to Handley's method, the cell surface was stained with ruthenium red even in the area where osteoblastic cells adjacent to the bone matrix were in close contact. The tissue penetration of ruthenium red, when prepared 3 hours previously, appears to be superior to one fixed with ruthenium red-OsO4 solution prepared just before use. This indicates that Handley's modified ruthenium red method is useful not only in the localization of mucopolysaccharide, but also in studies on the communication of the intracellular membrane system with the, outside, and as an intercellular tracer." @default.
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- W1963483977 date "1984-01-01" @default.
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- W1963483977 title "An application of modified ruthenium red staining for mouse calvaria." @default.
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- W1963483977 doi "https://doi.org/10.2330/joralbiosci1965.26.941" @default.
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