FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1963498172> ?p ?o ?g. }

• W1963498172 abstract "Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FLProstate-specific antigen (PSA or kallikrein-related peptidase 3, KLK3) is a chymotrypsin-like serine protease expressed in the prostate and secreted into seminal fluid. Several studies have demonstrated that PSA exerts anti-angiogenic activity in vitro and in vivo. Using a human umbilical vein endothelial cell (HUVEC) tube formation model, where the cells were grown on Matrigel™ basement membrane preparation, we recently showed that the enzymatic activity of PSA is essential for its anti-angiogenic function. Previously, PSA has been shown to cleave components of extracellular matrix (ECM), e.g., laminin and fibronectin, as well as unidentified proteins in Matrigel™. Proteolytic products of some ECM proteins, such as collagen XVIII-fragment endostatin, are known to be anti-angiogenic. Cleavage of ECM is also crucial for cancer cell invasion. We studied the PSA-generated protein fragments derived from Matrigel™ and aimed to characterize their anti-angiogenic activity.PSA was purified from seminal fluid and incubated with Matrigel™ for 24 h at 37°C. The resulting proteolytic fragments were analyzed by SDS-PAGE and mass spectrometry. Recombinant human nidogen-1 (also known as entactin) was incubated with PSA for 18 h at 37°C and the fragments were analyzed by SDS-PAGE followed by silver staining. The effect of nidogen-1 fragments was studied in the HUVEC tube formation model.We found that PSA cleaves nidogen-1 in Matrigel™. This was confirmed by using recombinant nidogen-1. The nidogen-1 fragments, however, did not have any anti-angiogenic effect on HUVECs in the tube formation model.We conclude that the cleavage of nidogen-1 by PSA may lead to disruption of basement membrane structure and, thus, may inhibit HUVEC tube formation as well as facilitate cancer cell invasion. The nidogen-1 fragments per se do not mediate the anti-angiogenic function of PSA.Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 1501. doi:10.1158/1538-7445.AM2011-1501" @default.
• W1963498172 created "2016-06-24" @default.
• W1963498172 creator A5000531928 @default.
• W1963498172 creator A5004015044 @default.
• W1963498172 creator A5065451296 @default.
• W1963498172 creator A5072489409 @default.
• W1963498172 date "2011-04-15" @default.
• W1963498172 modified "2023-09-25" @default.
• W1963498172 title "Abstract 1501: Prostate-specific antigen cleaves nidogen-1" @default.
• W1963498172 doi "https://doi.org/10.1158/1538-7445.am2011-1501" @default.
• W1963498172 hasPublicationYear "2011" @default.
• W1963498172 type Work @default.
• W1963498172 sameAs 1963498172 @default.
• W1963498172 citedByCount "0" @default.
• W1963498172 crossrefType "proceedings-article" @default.
• W1963498172 hasAuthorship W1963498172A5000531928 @default.
• W1963498172 hasAuthorship W1963498172A5004015044 @default.
• W1963498172 hasAuthorship W1963498172A5065451296 @default.
• W1963498172 hasAuthorship W1963498172A5072489409 @default.
• W1963498172 hasConcept C153911025 @default.
• W1963498172 hasConcept C181199279 @default.
• W1963498172 hasConcept C182220744 @default.
• W1963498172 hasConcept C185592680 @default.
• W1963498172 hasConcept C189165786 @default.
• W1963498172 hasConcept C202751555 @default.
• W1963498172 hasConcept C2778608917 @default.
• W1963498172 hasConcept C2779814568 @default.
• W1963498172 hasConcept C2780394083 @default.
• W1963498172 hasConcept C502942594 @default.
• W1963498172 hasConcept C55493867 @default.
• W1963498172 hasConcept C86492073 @default.
• W1963498172 hasConcept C86803240 @default.
• W1963498172 hasConcept C95444343 @default.
• W1963498172 hasConceptScore W1963498172C153911025 @default.
• W1963498172 hasConceptScore W1963498172C181199279 @default.
• W1963498172 hasConceptScore W1963498172C182220744 @default.
• W1963498172 hasConceptScore W1963498172C185592680 @default.
• W1963498172 hasConceptScore W1963498172C189165786 @default.
• W1963498172 hasConceptScore W1963498172C202751555 @default.
• W1963498172 hasConceptScore W1963498172C2778608917 @default.
• W1963498172 hasConceptScore W1963498172C2779814568 @default.
• W1963498172 hasConceptScore W1963498172C2780394083 @default.
• W1963498172 hasConceptScore W1963498172C502942594 @default.
• W1963498172 hasConceptScore W1963498172C55493867 @default.
• W1963498172 hasConceptScore W1963498172C86492073 @default.
• W1963498172 hasConceptScore W1963498172C86803240 @default.
• W1963498172 hasConceptScore W1963498172C95444343 @default.
• W1963498172 hasLocation W19634981721 @default.
• W1963498172 hasOpenAccess W1963498172 @default.
• W1963498172 hasPrimaryLocation W19634981721 @default.
• W1963498172 hasRelatedWork W1531738684 @default.
• W1963498172 hasRelatedWork W1581797720 @default.
• W1963498172 hasRelatedWork W1941829554 @default.
• W1963498172 hasRelatedWork W1977874890 @default.
• W1963498172 hasRelatedWork W1983703392 @default.
• W1963498172 hasRelatedWork W2024656166 @default.
• W1963498172 hasRelatedWork W2027700219 @default.
• W1963498172 hasRelatedWork W2054037466 @default.
• W1963498172 hasRelatedWork W2066572325 @default.
• W1963498172 hasRelatedWork W2067901911 @default.
• W1963498172 hasRelatedWork W2071293631 @default.
• W1963498172 hasRelatedWork W2074715582 @default.
• W1963498172 hasRelatedWork W2074751736 @default.
• W1963498172 hasRelatedWork W2077505981 @default.
• W1963498172 hasRelatedWork W2082627308 @default.
• W1963498172 hasRelatedWork W2140406778 @default.
• W1963498172 hasRelatedWork W2141488374 @default.
• W1963498172 hasRelatedWork W2413116377 @default.
• W1963498172 hasRelatedWork W2802125325 @default.
• W1963498172 hasRelatedWork W2978691818 @default.
• W1963498172 isParatext "false" @default.
• W1963498172 isRetracted "false" @default.
• W1963498172 magId "1963498172" @default.
• W1963498172 workType "article" @default.