Matches in SemOpenAlex for { <https://semopenalex.org/work/W1963501900> ?p ?o ?g. }
- W1963501900 endingPage "6655" @default.
- W1963501900 startingPage "6655" @default.
- W1963501900 abstract "Purpose.: The ubiquitin–proteasome pathway (UPP) is an important protein quality control mechanism for selective degradation of abnormal proteins. The objective of this study was to test the hypothesis that enhancement of the UPP capacity could attenuate the accumulation and aggregation of misfolded proteins using V76D-γD-crystallin as a model substrate. Methods.: Wild type (wt) and V76D mutant γD-crystallin were fused to red fluorescence protein (RFP) and expressed in human lens epithelial cells. The cellular distribution of the expressed proteins was compared by fluorescence microscopy. The solubility of wt- and V76D-γD-crystallin was determined by cellular fractionation and Western blotting. Wt-γD-RFP and V76D-γD-RFP were also cotransfected along with a ubiquitin ligase (CHIP) or a ubiquitin-conjugating enzyme (Ubc5) into cells. Levels of wt- and V76D-γD-crystallin, the percentage of transfected cells with aggregates, and aggregate size were quantified and compared among different groups. Results.: Wt-γD-crystallin was evenly distributed in cells, whereas V76D-γD-crystallin formed intracellular aggregates. Eighty percent of wt-γD-crystallin was detected in the soluble fraction, whereas only 7% of V76D-γD-crystallin was soluble. CHIP or Ubc5 coexpression reduced the protein level of V76D-γD and concomitantly its aggregation in transfected cells; these effects could be attenuated by proteasome inhibitor. Mutant CHIP with defect TPR (tetratricopeptide repeat) or U-box domain failed to reduce levels of V76D-γD-crystallin. Conclusions.: Enhancing ubiquitin conjugation activity reduces accumulation and aggregation of V76D-γD-crystallin by promoting its degradation. Upregulation of ubiquitin-conjugating activity could be an effective strategy to maintain lens transparency by eliminating other forms of misfolded proteins." @default.
- W1963501900 created "2016-06-24" @default.
- W1963501900 creator A5003459470 @default.
- W1963501900 creator A5024915953 @default.
- W1963501900 creator A5027371446 @default.
- W1963501900 creator A5049594879 @default.
- W1963501900 creator A5068775517 @default.
- W1963501900 creator A5077006581 @default.
- W1963501900 date "2012-09-25" @default.
- W1963501900 modified "2023-09-22" @default.
- W1963501900 title "Enhancement of Ubiquitin Conjugation Activity Reduces Intracellular Aggregation of V76D Mutant γD-Crystallin" @default.
- W1963501900 cites W1520171546 @default.
- W1963501900 cites W1549578642 @default.
- W1963501900 cites W1552448470 @default.
- W1963501900 cites W1574357584 @default.
- W1963501900 cites W1582124175 @default.
- W1963501900 cites W1592407422 @default.
- W1963501900 cites W1851814176 @default.
- W1963501900 cites W1964021682 @default.
- W1963501900 cites W1975996615 @default.
- W1963501900 cites W1976985900 @default.
- W1963501900 cites W1980824786 @default.
- W1963501900 cites W1983959956 @default.
- W1963501900 cites W1985549353 @default.
- W1963501900 cites W1986906983 @default.
- W1963501900 cites W1992341964 @default.
- W1963501900 cites W2002104809 @default.
- W1963501900 cites W2002559713 @default.
- W1963501900 cites W2004285146 @default.
- W1963501900 cites W2006625206 @default.
- W1963501900 cites W2008264362 @default.
- W1963501900 cites W2008726342 @default.
- W1963501900 cites W2008751067 @default.
- W1963501900 cites W2009890105 @default.
- W1963501900 cites W2010950084 @default.
- W1963501900 cites W2012263859 @default.
- W1963501900 cites W2012296420 @default.
- W1963501900 cites W2013339424 @default.
- W1963501900 cites W2013344195 @default.
- W1963501900 cites W2014765226 @default.
- W1963501900 cites W2015357935 @default.
- W1963501900 cites W2016686667 @default.
- W1963501900 cites W2018462246 @default.
- W1963501900 cites W2021605503 @default.
- W1963501900 cites W2024794859 @default.
- W1963501900 cites W2031259748 @default.
- W1963501900 cites W2034137333 @default.
- W1963501900 cites W2034537039 @default.
- W1963501900 cites W2035191977 @default.
- W1963501900 cites W2037279407 @default.
- W1963501900 cites W2040743789 @default.
- W1963501900 cites W2057033170 @default.
- W1963501900 cites W2058469726 @default.
- W1963501900 cites W2059617452 @default.
- W1963501900 cites W2060051476 @default.
- W1963501900 cites W2060631752 @default.
- W1963501900 cites W2060834185 @default.
- W1963501900 cites W2063641892 @default.
- W1963501900 cites W2067852794 @default.
- W1963501900 cites W2068550859 @default.
- W1963501900 cites W2068940242 @default.
- W1963501900 cites W2069183580 @default.
- W1963501900 cites W2072979868 @default.
- W1963501900 cites W2095134835 @default.
- W1963501900 cites W2097094654 @default.
- W1963501900 cites W2098076627 @default.
- W1963501900 cites W2107442883 @default.
- W1963501900 cites W2113214792 @default.
- W1963501900 cites W2113461670 @default.
- W1963501900 cites W2113850239 @default.
- W1963501900 cites W2125774668 @default.
- W1963501900 cites W2132292213 @default.
- W1963501900 cites W2143624016 @default.
- W1963501900 cites W2143659623 @default.
- W1963501900 cites W2144385780 @default.
- W1963501900 cites W2145101544 @default.
- W1963501900 cites W2146149989 @default.
- W1963501900 cites W2150123812 @default.
- W1963501900 cites W2150368867 @default.
- W1963501900 cites W2150462237 @default.
- W1963501900 cites W2161898117 @default.
- W1963501900 cites W2166987747 @default.
- W1963501900 cites W2176799918 @default.
- W1963501900 cites W69243547 @default.
- W1963501900 doi "https://doi.org/10.1167/iovs.12-9744" @default.
- W1963501900 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3460391" @default.
- W1963501900 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22915036" @default.
- W1963501900 hasPublicationYear "2012" @default.
- W1963501900 type Work @default.
- W1963501900 sameAs 1963501900 @default.
- W1963501900 citedByCount "7" @default.
- W1963501900 countsByYear W19635019002017 @default.
- W1963501900 countsByYear W19635019002018 @default.
- W1963501900 countsByYear W19635019002020 @default.
- W1963501900 countsByYear W19635019002021 @default.
- W1963501900 countsByYear W19635019002023 @default.
- W1963501900 crossrefType "journal-article" @default.
- W1963501900 hasAuthorship W1963501900A5003459470 @default.