FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1963510459> ?p ?o ?g. }

• W1963510459 abstract "Prostate-specific antigen (PSA) is the most extensively used biomarker in cancer. There continues to be intense debate regarding the use of PSA in screening for prostate cancer (PCa) and how it may be used for optimal benefit. The role PSA plays in reproductive biology and PCa has been an area of ongoing interest. As a serine protease with novel substrate preferences, PSA continues to be secreted at high levels (mg/mL) even by the majority of advanced prostatic carcinomas. This protease has been shown to affect the growth or viability of a variety of cell types, including epithelial cells, osteoclasts, T-cells, and human PCa cell lines. In this study, we stably reduced endogenous PSA gene expression using lentiviral short hairpin loop RNA (shRNA) technology in the LNCaP human PCa cell line. We then investigated any resulting changes to LNCaP growth rates in vitro and ex vivo. Acting on the hypothesis that any PSA-induced changes to LNCaP behavior are the result of the enzyme9s extracellular activity, we used a high-throughput shot-gun mass spectrometry-based (MS) approach to analyze the in vitro extracellular LNCaP proteome in control and PSA-targeted shRNA populations. Having demonstrated that our shRNA strategy targeted to PSA caused as much as a 90% reduction in PSA protein production compared to control cells, we next showed that in vitro doubling times are slowed from 2 days to 11 days. Subcutaneous tumors displayed an almost 50% reduction in take rate, with the final weights of those remaining only reaching 10% of controls after 8 weeks of growth. Serum-free media conditioned for 24 hours by control or PSA-targeted LNCaP populations were then subjected to global semi-quantitative proteomics analysis using the high resolution LTQ-Orbitrap mass spectrometer (Thermo Electron, San Jose, CA). In one 200 minute LTQ-Orbitrap analysis, in conjunction with the Mascot Search algorithm (Matrix Science, Boston, MA), we were able to identify 1784 proteins in the LNCaP conditioned media. Of these, 686 were specific to the control populations, 527 were specific to the reduced PSA populations, and 571 were common to both. These results demonstrate the utility of high-throughput proteomic strategies as an unbiased approach for the identification of candidate mechanisms behind novel observations in cancer biology. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2326." @default.
• W1963510459 created "2016-06-24" @default.
• W1963510459 creator A5001771354 @default.
• W1963510459 creator A5043265453 @default.
• W1963510459 creator A5079988714 @default.
• W1963510459 creator A5084043720 @default.
• W1963510459 creator A5088662632 @default.
• W1963510459 date "2010-04-15" @default.
• W1963510459 modified "2023-10-03" @default.
• W1963510459 title "Abstract 2326: Loss of prostate-specific antigen slows growth and alters the extracellular proteome of LNCaP human prostate cancer cells" @default.
• W1963510459 doi "https://doi.org/10.1158/1538-7445.am10-2326" @default.
• W1963510459 hasPublicationYear "2010" @default.
• W1963510459 type Work @default.
• W1963510459 sameAs 1963510459 @default.
• W1963510459 citedByCount "0" @default.
• W1963510459 crossrefType "proceedings-article" @default.
• W1963510459 hasAuthorship W1963510459A5001771354 @default.
• W1963510459 hasAuthorship W1963510459A5043265453 @default.
• W1963510459 hasAuthorship W1963510459A5079988714 @default.
• W1963510459 hasAuthorship W1963510459A5084043720 @default.
• W1963510459 hasAuthorship W1963510459A5088662632 @default.
• W1963510459 hasConcept C104317684 @default.
• W1963510459 hasConcept C121608353 @default.
• W1963510459 hasConcept C126322002 @default.
• W1963510459 hasConcept C145059251 @default.
• W1963510459 hasConcept C173396325 @default.
• W1963510459 hasConcept C185592680 @default.
• W1963510459 hasConcept C190232843 @default.
• W1963510459 hasConcept C20518536 @default.
• W1963510459 hasConcept C2779723316 @default.
• W1963510459 hasConcept C2780192828 @default.
• W1963510459 hasConcept C2781406297 @default.
• W1963510459 hasConcept C2908689518 @default.
• W1963510459 hasConcept C502942594 @default.
• W1963510459 hasConcept C54355233 @default.
• W1963510459 hasConcept C55493867 @default.
• W1963510459 hasConcept C71924100 @default.
• W1963510459 hasConcept C81885089 @default.
• W1963510459 hasConcept C86803240 @default.
• W1963510459 hasConceptScore W1963510459C104317684 @default.
• W1963510459 hasConceptScore W1963510459C121608353 @default.
• W1963510459 hasConceptScore W1963510459C126322002 @default.
• W1963510459 hasConceptScore W1963510459C145059251 @default.
• W1963510459 hasConceptScore W1963510459C173396325 @default.
• W1963510459 hasConceptScore W1963510459C185592680 @default.
• W1963510459 hasConceptScore W1963510459C190232843 @default.
• W1963510459 hasConceptScore W1963510459C20518536 @default.
• W1963510459 hasConceptScore W1963510459C2779723316 @default.
• W1963510459 hasConceptScore W1963510459C2780192828 @default.
• W1963510459 hasConceptScore W1963510459C2781406297 @default.
• W1963510459 hasConceptScore W1963510459C2908689518 @default.
• W1963510459 hasConceptScore W1963510459C502942594 @default.
• W1963510459 hasConceptScore W1963510459C54355233 @default.
• W1963510459 hasConceptScore W1963510459C55493867 @default.
• W1963510459 hasConceptScore W1963510459C71924100 @default.
• W1963510459 hasConceptScore W1963510459C81885089 @default.
• W1963510459 hasConceptScore W1963510459C86803240 @default.
• W1963510459 hasLocation W19635104591 @default.
• W1963510459 hasOpenAccess W1963510459 @default.
• W1963510459 hasPrimaryLocation W19635104591 @default.
• W1963510459 hasRelatedWork W1026215867 @default.
• W1963510459 hasRelatedWork W1485208459 @default.
• W1963510459 hasRelatedWork W2004123655 @default.
• W1963510459 hasRelatedWork W2017135494 @default.
• W1963510459 hasRelatedWork W2022657920 @default.
• W1963510459 hasRelatedWork W2040829845 @default.
• W1963510459 hasRelatedWork W2136160453 @default.
• W1963510459 hasRelatedWork W2167891683 @default.
• W1963510459 hasRelatedWork W2290591211 @default.
• W1963510459 hasRelatedWork W2314955586 @default.
• W1963510459 hasRelatedWork W2325289331 @default.
• W1963510459 hasRelatedWork W2329477774 @default.
• W1963510459 hasRelatedWork W2334177911 @default.
• W1963510459 hasRelatedWork W2371500577 @default.
• W1963510459 hasRelatedWork W2372172309 @default.
• W1963510459 hasRelatedWork W2801433010 @default.
• W1963510459 hasRelatedWork W2979163445 @default.
• W1963510459 hasRelatedWork W3045818159 @default.
• W1963510459 hasRelatedWork W3081539624 @default.
• W1963510459 hasRelatedWork W3125761770 @default.
• W1963510459 isParatext "false" @default.
• W1963510459 isRetracted "false" @default.
• W1963510459 magId "1963510459" @default.
• W1963510459 workType "article" @default.