Matches in SemOpenAlex for { <https://semopenalex.org/work/W1963514012> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W1963514012 endingPage "457" @default.
- W1963514012 startingPage "450" @default.
- W1963514012 abstract "A microscope which can measure the mean fluorescence lifetimes of small fluorescent samples is described. Special emphasis is given to fluorochromes dissolved in biological solutions and bound to thin polymeric substances, such as the desoxyribonucleic acid complexes present in the nuclei of cells. Fundamentally, the method is similar to that described by physicists and physicochemists for solutions of fluorochrome only. A diffraction modulator is used to sinusoidally modulate the intensity of a high-pressure mercury-vapor lamp. The phase shift between the unabsorbed exciting light, passing through the microscopic sample, and the fluorescent light is related to the fluorescence lifetime by tanΔφ=ωτ. The phase shift Δφ is obtained differentially from the phase angles φ1 and φ2 of each microscopic light signal with respect to a third less noisy signal, the tracer signal. The principal effort has been directed toward studying the various sources of error in the fluorometer and variabilities in lifetime measurements. The data indicate that the mean fluorescence lifetime (2.7 msec) of proflavine, bound to nuclei of tumor cells and observed with a 130× oil immersion lens, can be determined with a precision of 4×10−10 sec." @default.
- W1963514012 created "2016-06-24" @default.
- W1963514012 creator A5044130415 @default.
- W1963514012 date "1959-06-01" @default.
- W1963514012 modified "2023-09-28" @default.
- W1963514012 title "Microscope Phase Fluorometer for Determining the Fluorescence Lifetimes of Fluorochromes" @default.
- W1963514012 cites W2083892970 @default.
- W1963514012 cites W2281067309 @default.
- W1963514012 doi "https://doi.org/10.1063/1.1716652" @default.
- W1963514012 hasPublicationYear "1959" @default.
- W1963514012 type Work @default.
- W1963514012 sameAs 1963514012 @default.
- W1963514012 citedByCount "46" @default.
- W1963514012 countsByYear W19635140122012 @default.
- W1963514012 countsByYear W19635140122014 @default.
- W1963514012 countsByYear W19635140122015 @default.
- W1963514012 countsByYear W19635140122017 @default.
- W1963514012 countsByYear W19635140122018 @default.
- W1963514012 countsByYear W19635140122020 @default.
- W1963514012 countsByYear W19635140122021 @default.
- W1963514012 countsByYear W19635140122022 @default.
- W1963514012 countsByYear W19635140122023 @default.
- W1963514012 crossrefType "journal-article" @default.
- W1963514012 hasAuthorship W1963514012A5044130415 @default.
- W1963514012 hasConcept C113196181 @default.
- W1963514012 hasConcept C120665830 @default.
- W1963514012 hasConcept C121332964 @default.
- W1963514012 hasConcept C147080431 @default.
- W1963514012 hasConcept C169274487 @default.
- W1963514012 hasConcept C177634673 @default.
- W1963514012 hasConcept C178790620 @default.
- W1963514012 hasConcept C185592680 @default.
- W1963514012 hasConcept C192562407 @default.
- W1963514012 hasConcept C199360897 @default.
- W1963514012 hasConcept C202800777 @default.
- W1963514012 hasConcept C26771246 @default.
- W1963514012 hasConcept C2779843651 @default.
- W1963514012 hasConcept C41008148 @default.
- W1963514012 hasConcept C43617362 @default.
- W1963514012 hasConcept C44280652 @default.
- W1963514012 hasConcept C67649825 @default.
- W1963514012 hasConcept C77017923 @default.
- W1963514012 hasConcept C91881484 @default.
- W1963514012 hasConceptScore W1963514012C113196181 @default.
- W1963514012 hasConceptScore W1963514012C120665830 @default.
- W1963514012 hasConceptScore W1963514012C121332964 @default.
- W1963514012 hasConceptScore W1963514012C147080431 @default.
- W1963514012 hasConceptScore W1963514012C169274487 @default.
- W1963514012 hasConceptScore W1963514012C177634673 @default.
- W1963514012 hasConceptScore W1963514012C178790620 @default.
- W1963514012 hasConceptScore W1963514012C185592680 @default.
- W1963514012 hasConceptScore W1963514012C192562407 @default.
- W1963514012 hasConceptScore W1963514012C199360897 @default.
- W1963514012 hasConceptScore W1963514012C202800777 @default.
- W1963514012 hasConceptScore W1963514012C26771246 @default.
- W1963514012 hasConceptScore W1963514012C2779843651 @default.
- W1963514012 hasConceptScore W1963514012C41008148 @default.
- W1963514012 hasConceptScore W1963514012C43617362 @default.
- W1963514012 hasConceptScore W1963514012C44280652 @default.
- W1963514012 hasConceptScore W1963514012C67649825 @default.
- W1963514012 hasConceptScore W1963514012C77017923 @default.
- W1963514012 hasConceptScore W1963514012C91881484 @default.
- W1963514012 hasIssue "6" @default.
- W1963514012 hasLocation W19635140121 @default.
- W1963514012 hasOpenAccess W1963514012 @default.
- W1963514012 hasPrimaryLocation W19635140121 @default.
- W1963514012 hasRelatedWork W1038967776 @default.
- W1963514012 hasRelatedWork W1598789855 @default.
- W1963514012 hasRelatedWork W1602037374 @default.
- W1963514012 hasRelatedWork W1999333779 @default.
- W1963514012 hasRelatedWork W2001993321 @default.
- W1963514012 hasRelatedWork W2010819146 @default.
- W1963514012 hasRelatedWork W2017240297 @default.
- W1963514012 hasRelatedWork W2032380809 @default.
- W1963514012 hasRelatedWork W2046252844 @default.
- W1963514012 hasRelatedWork W2048413443 @default.
- W1963514012 hasVolume "30" @default.
- W1963514012 isParatext "false" @default.
- W1963514012 isRetracted "false" @default.
- W1963514012 magId "1963514012" @default.
- W1963514012 workType "article" @default.