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• W1963516708 abstract "Epidermal cells from pigmented areas of a patient with vitiligo were cultured in MCDB-153 medium, which supports the clonal growth of undifferentiated keratinocytes and melanocytes. The cells were grown on collagen-coated substrata. After the cells reached semiconfluence, the composite of substratum and cells was emplaced onto dermabraded vitiliginous areas as a graft. Re-epithelialization of the grafted areas was complete after 2 weeks. Repigmentation was evident after 1 month and continued over the observation period of several months. There was complete and normal differentiation of the graft, including a normal distribution of melanocytes in the basal layer. Ultrastructural studies showed a normal distribution of melanosomes in the melanocytes and showed keratinocytes that were indistinguishable from the uninvolved skin." @default.
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• W1963516708 date "1989-05-01" @default.
• W1963516708 modified "2023-10-14" @default.
• W1963516708 title "Repigmentation of Vitiliginous Skin by Cultured Cells" @default.
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• W1963516708 doi "https://doi.org/10.1111/j.1600-0749.1989.tb00186.x" @default.
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