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• W1963525308 abstract "<i>Brettanomyces/Dekkera</i> is considered as one of the main causes of microbial spoilage and sensory deviations of red wines. This work compares the sensitivity and effectiveness of conventional microbiological culture and real-time polymerase chain reaction (Q-PCR) methods for <i>Brettanomyces/Dekkera</i> detection and quantification and demonstrates a positive correlation between both methods. Moreover, an improved DNA extraction protocol enabled quantification of <i>Brettanomyces/Dekkera</i> cells by Q-PCR down to 20 cells/mL in turbid wines in a total of 324 red wine samples. The conventional culture analysis is time-consuming but has lower cost than Q-PCR, and it is simple and efficient in quantifying viable <i>Brettanomyces/Dekkera</i> cells." @default.
• W1963525308 created "2016-06-24" @default.
• W1963525308 creator A5023224657 @default.
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• W1963525308 date "2012-10-22" @default.
• W1963525308 modified "2023-10-17" @default.
• W1963525308 title "Comparison of Specific Real-Time PCR and Conventional Culture for Detection and Enumeration of Brettanomyces in Red Wines" @default.
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• W1963525308 doi "https://doi.org/10.5344/ajev.2012.12047" @default.
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