FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1963529285> ?p ?o ?g. }

• W1963529285 endingPage "837" @default.
• W1963529285 startingPage "829" @default.
• W1963529285 abstract "Background The major house dust mite allergen Der p 2 has been regarded as an important allergen involved in the immunopathogenesis of allergic asthma and eczema. Objectives To determine the degree of sequence polymorphism exists in Der p 2 at both the genomic DNA and cDNA levels. Methods Isolation of cDNA clones was performed by screening the cDNA libraries with Der p 2 cDNA probe and the genomic sequences for Der p 2 were obtained by PCR amplification from environmental dust mites with Der p 2-specific primers. DNA sequencing was carried out by the dideoxynucleotide chain termination method. The study of Der p 2 isoforms was performed by 2-D gel immunoblot analysis using mouse anti-Der p 2 serum. Results In this study, we have characterized the seqtiences of three difierent genealleles coding for the major house dust mite allergen Der p 2 at the cDNA level. The translated polypeptides from these clones differed from each other by 3–4 amino-acid residues. These polymorphic residues determined were also found in Der f 2 and they were located in regions containing T-epitopes. In addition, the genomic DNA sequences of Der p 2 which were obtained by PCR amplification using the environmental mites isolated from Taiwan and Australia have helped to confirm the authenticity of the polymorphisms detected in the cDNA clones generated from CSL cultured mites. Furthermore, 2D- immunoblot analysis indicated that there were at least 10 different isoforms (p 1 values range from greater than 7.0–5.9) of Der p 2 proteins produced by CSL cultured mites. Conclusion The results showed that there was a small but significant degree of sequence polymorphisms exists in Der p 2 gene alleles. Interestingly, the polymorphic residues were found in regions containing previously determined T-epitopes. The polymorphism data reported here will be important for the understanding of the immune responses of mite allergens as well as for the development of the peptide-based immunotherapeutic reagents for mite allergy." @default.
• W1963529285 created "2016-06-24" @default.
• W1963529285 creator A5007496033 @default.
• W1963529285 creator A5012185249 @default.
• W1963529285 creator A5046205006 @default.
• W1963529285 creator A5065961454 @default.
• W1963529285 date "1996-07-01" @default.
• W1963529285 modified "2023-10-16" @default.
• W1963529285 title "Analysis of sequence polymorphism of a major mite allergen, Der p 2" @default.
• W1963529285 cites W1509108153 @default.
• W1963529285 cites W1601687245 @default.
• W1963529285 cites W1925788296 @default.
• W1963529285 cites W1965074258 @default.
• W1963529285 cites W1965584649 @default.
• W1963529285 cites W1983217968 @default.
• W1963529285 cites W1983714532 @default.
• W1963529285 cites W2001718785 @default.
• W1963529285 cites W2001951316 @default.
• W1963529285 cites W2005310437 @default.
• W1963529285 cites W2024249282 @default.
• W1963529285 cites W2036759647 @default.
• W1963529285 cites W2066203499 @default.
• W1963529285 cites W2068088837 @default.
• W1963529285 cites W2077307767 @default.
• W1963529285 cites W2090687924 @default.
• W1963529285 cites W2111583006 @default.
• W1963529285 cites W2124620276 @default.
• W1963529285 cites W2138270253 @default.
• W1963529285 cites W2169581200 @default.
• W1963529285 cites W2462890753 @default.
• W1963529285 doi "https://doi.org/10.1111/j.1365-2222.1996.tb00615.x" @default.
• W1963529285 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8842558" @default.
• W1963529285 hasPublicationYear "1996" @default.
• W1963529285 type Work @default.
• W1963529285 sameAs 1963529285 @default.
• W1963529285 citedByCount "47" @default.
• W1963529285 countsByYear W19635292852012 @default.
• W1963529285 countsByYear W19635292852013 @default.
• W1963529285 countsByYear W19635292852014 @default.
• W1963529285 countsByYear W19635292852015 @default.
• W1963529285 countsByYear W19635292852017 @default.
• W1963529285 countsByYear W19635292852019 @default.
• W1963529285 countsByYear W19635292852022 @default.
• W1963529285 crossrefType "journal-article" @default.
• W1963529285 hasAuthorship W1963529285A5007496033 @default.
• W1963529285 hasAuthorship W1963529285A5012185249 @default.
• W1963529285 hasAuthorship W1963529285A5046205006 @default.
• W1963529285 hasAuthorship W1963529285A5065961454 @default.
• W1963529285 hasConcept C104317684 @default.
• W1963529285 hasConcept C153911025 @default.
• W1963529285 hasConcept C17757408 @default.
• W1963529285 hasConcept C187882448 @default.
• W1963529285 hasConcept C203014093 @default.
• W1963529285 hasConcept C207480886 @default.
• W1963529285 hasConcept C2778166374 @default.
• W1963529285 hasConcept C2779673145 @default.
• W1963529285 hasConcept C2779711295 @default.
• W1963529285 hasConcept C2780510475 @default.
• W1963529285 hasConcept C49105822 @default.
• W1963529285 hasConcept C54355233 @default.
• W1963529285 hasConcept C552990157 @default.
• W1963529285 hasConcept C59822182 @default.
• W1963529285 hasConcept C61053724 @default.
• W1963529285 hasConcept C86803240 @default.
• W1963529285 hasConceptScore W1963529285C104317684 @default.
• W1963529285 hasConceptScore W1963529285C153911025 @default.
• W1963529285 hasConceptScore W1963529285C17757408 @default.
• W1963529285 hasConceptScore W1963529285C187882448 @default.
• W1963529285 hasConceptScore W1963529285C203014093 @default.
• W1963529285 hasConceptScore W1963529285C207480886 @default.
• W1963529285 hasConceptScore W1963529285C2778166374 @default.
• W1963529285 hasConceptScore W1963529285C2779673145 @default.
• W1963529285 hasConceptScore W1963529285C2779711295 @default.
• W1963529285 hasConceptScore W1963529285C2780510475 @default.
• W1963529285 hasConceptScore W1963529285C49105822 @default.
• W1963529285 hasConceptScore W1963529285C54355233 @default.
• W1963529285 hasConceptScore W1963529285C552990157 @default.
• W1963529285 hasConceptScore W1963529285C59822182 @default.
• W1963529285 hasConceptScore W1963529285C61053724 @default.
• W1963529285 hasConceptScore W1963529285C86803240 @default.
• W1963529285 hasIssue "7" @default.
• W1963529285 hasLocation W19635292851 @default.
• W1963529285 hasLocation W19635292852 @default.
• W1963529285 hasOpenAccess W1963529285 @default.
• W1963529285 hasPrimaryLocation W19635292851 @default.
• W1963529285 hasRelatedWork W2016348400 @default.
• W1963529285 hasRelatedWork W2027547758 @default.
• W1963529285 hasRelatedWork W2036914727 @default.
• W1963529285 hasRelatedWork W2043741683 @default.
• W1963529285 hasRelatedWork W2048349747 @default.
• W1963529285 hasRelatedWork W2054600043 @default.
• W1963529285 hasRelatedWork W2174587448 @default.
• W1963529285 hasRelatedWork W2316813213 @default.
• W1963529285 hasRelatedWork W1975653399 @default.
• W1963529285 hasRelatedWork W3152185625 @default.
• W1963529285 hasVolume "26" @default.
• W1963529285 isParatext "false" @default.
• W1963529285 isRetracted "false" @default.

• next