Matches in SemOpenAlex for { <https://semopenalex.org/work/W1963530359> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W1963530359 endingPage "331" @default.
- W1963530359 startingPage "324" @default.
- W1963530359 abstract "Acetylcholine (ACh), synthesized in the pituitary, can act locally to modulate pituitary function. We used rat primary anterior pituitary (AP) cells to investigate how ACh affects pituitary prolactin (PRL) secretion in the presence or absence of known PRL regulators: thyrotropin-releasing hormone (TRH), 17beta-estradiol (E(2)) and triiodothyronine (T(3)). Cultured AP cells were prepared from ovariectomized rats and pretreated with diluent, 0.6 nM E(2), 10 nM T(3), or E(2) plus T(3) for 5 days, then challenged with various doses of ACh or muscarinic receptor agonists (oxotremorine or carbachol) and TRH (100 nM) for 20 min. Significant ACh (10(-5) M) suppression of both basal and TRH-induced PRL secretion was not evident in diluent-, E(2)- or T(3)-pretreated cells, but observed only in cells pretreated with both E(2) and T(3). Moreover, in E(2) plus T(3)-pretreated cells, oxotremorine and carbachol, like ACh (10(-7)-10(-5) M), suppressed both responses in a dose- related manner. Pertussis toxin (PTX; 100 ng/ml) as well as atropine (a muscarinic receptor antagonist; 1 mM) blocked these effects of cholinomimetics. ACh also inhibited both PRL responses elicited by drugs elevating intracellular cAMP (10 microM forskolin) or Ca(2+) (1 microM Bay K-8644) in a PTX-sensitive manner. ACh inhibition of basal PRL secretion was unaltered by intracellular Ca(2+) mobilization blockers, TMB-8 (100 microM) and thapsigargin (1 microM), but abrogated by the nitric oxide synthase inhibitor (300 microM L-NAME). ACh inhibition of TRH-induced PRL secretion was accentuated by TMB-8 and alleviated by thapsigargin or L-NAME. In summary, muscarinic inhibition of either basal or TRH-induced PRL secretion was augmented by E(2) and T(3), and involved the PTX-sensitive cAMP/Ca(2+) pathways. Furthermore, nitric oxide mediated the basal rather than TRH-induced PRL response to ACh, whereas the intracellular Ca(2+) mobilization concerned the TRH-induced rather than the basal PRL response to ACh. Thus, ACh synthesized in the AP appears to inhibit basal vs. TRH-induced PRL secretion via different mechanisms." @default.
- W1963530359 created "2016-06-24" @default.
- W1963530359 creator A5008522874 @default.
- W1963530359 creator A5043283337 @default.
- W1963530359 creator A5057366774 @default.
- W1963530359 creator A5076564117 @default.
- W1963530359 creator A5089948999 @default.
- W1963530359 date "1999-01-01" @default.
- W1963530359 modified "2023-10-14" @default.
- W1963530359 title "Muscarinic Regulation of Basal versus Thyrotropin-Releasing Hormone-Induced Prolactin Secretion in Rat AnteriorPituitary Cells" @default.
- W1963530359 cites W1986784184 @default.
- W1963530359 cites W2035452415 @default.
- W1963530359 cites W2040433201 @default.
- W1963530359 cites W2044113041 @default.
- W1963530359 cites W2050432697 @default.
- W1963530359 cites W2077927873 @default.
- W1963530359 cites W2084791647 @default.
- W1963530359 cites W2094504936 @default.
- W1963530359 cites W4235966843 @default.
- W1963530359 doi "https://doi.org/10.1159/000054493" @default.
- W1963530359 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10567858" @default.
- W1963530359 hasPublicationYear "1999" @default.
- W1963530359 type Work @default.
- W1963530359 sameAs 1963530359 @default.
- W1963530359 citedByCount "5" @default.
- W1963530359 countsByYear W19635303592012 @default.
- W1963530359 countsByYear W19635303592018 @default.
- W1963530359 crossrefType "journal-article" @default.
- W1963530359 hasAuthorship W1963530359A5008522874 @default.
- W1963530359 hasAuthorship W1963530359A5043283337 @default.
- W1963530359 hasAuthorship W1963530359A5057366774 @default.
- W1963530359 hasAuthorship W1963530359A5076564117 @default.
- W1963530359 hasAuthorship W1963530359A5089948999 @default.
- W1963530359 hasConcept C116289061 @default.
- W1963530359 hasConcept C126322002 @default.
- W1963530359 hasConcept C134018914 @default.
- W1963530359 hasConcept C170493617 @default.
- W1963530359 hasConcept C185592680 @default.
- W1963530359 hasConcept C207723603 @default.
- W1963530359 hasConcept C24998067 @default.
- W1963530359 hasConcept C2775910092 @default.
- W1963530359 hasConcept C2776216936 @default.
- W1963530359 hasConcept C2778824757 @default.
- W1963530359 hasConcept C2778988552 @default.
- W1963530359 hasConcept C2779064019 @default.
- W1963530359 hasConcept C2779600325 @default.
- W1963530359 hasConcept C2780841807 @default.
- W1963530359 hasConcept C33789571 @default.
- W1963530359 hasConcept C71315377 @default.
- W1963530359 hasConcept C71924100 @default.
- W1963530359 hasConcept C86803240 @default.
- W1963530359 hasConceptScore W1963530359C116289061 @default.
- W1963530359 hasConceptScore W1963530359C126322002 @default.
- W1963530359 hasConceptScore W1963530359C134018914 @default.
- W1963530359 hasConceptScore W1963530359C170493617 @default.
- W1963530359 hasConceptScore W1963530359C185592680 @default.
- W1963530359 hasConceptScore W1963530359C207723603 @default.
- W1963530359 hasConceptScore W1963530359C24998067 @default.
- W1963530359 hasConceptScore W1963530359C2775910092 @default.
- W1963530359 hasConceptScore W1963530359C2776216936 @default.
- W1963530359 hasConceptScore W1963530359C2778824757 @default.
- W1963530359 hasConceptScore W1963530359C2778988552 @default.
- W1963530359 hasConceptScore W1963530359C2779064019 @default.
- W1963530359 hasConceptScore W1963530359C2779600325 @default.
- W1963530359 hasConceptScore W1963530359C2780841807 @default.
- W1963530359 hasConceptScore W1963530359C33789571 @default.
- W1963530359 hasConceptScore W1963530359C71315377 @default.
- W1963530359 hasConceptScore W1963530359C71924100 @default.
- W1963530359 hasConceptScore W1963530359C86803240 @default.
- W1963530359 hasIssue "5" @default.
- W1963530359 hasLocation W19635303591 @default.
- W1963530359 hasLocation W19635303592 @default.
- W1963530359 hasOpenAccess W1963530359 @default.
- W1963530359 hasPrimaryLocation W19635303591 @default.
- W1963530359 hasRelatedWork W1968051627 @default.
- W1963530359 hasRelatedWork W2002021490 @default.
- W1963530359 hasRelatedWork W203838852 @default.
- W1963530359 hasRelatedWork W2042321847 @default.
- W1963530359 hasRelatedWork W2043607792 @default.
- W1963530359 hasRelatedWork W2085804130 @default.
- W1963530359 hasRelatedWork W2090550284 @default.
- W1963530359 hasRelatedWork W2121872275 @default.
- W1963530359 hasRelatedWork W2141515004 @default.
- W1963530359 hasRelatedWork W2146001153 @default.
- W1963530359 hasVolume "70" @default.
- W1963530359 isParatext "false" @default.
- W1963530359 isRetracted "false" @default.
- W1963530359 magId "1963530359" @default.
- W1963530359 workType "article" @default.