FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1963537728> ?p ?o ?g. }

• W1963537728 endingPage "395" @default.
• W1963537728 startingPage "395" @default.
• W1963537728 abstract "2-Chloro-10-[3(-dimethylamino)propyl]phenothiazinemonohydrochloride (chlorpromazine) is a phenothiazine derivative used clinically to control psychotic disorders. It also exhibits an anticancer activity. Treatment with chlorpromazine (CPZ) results in cell-cycle arrest at the G2/M phase in rat C6 glioma cells. CPZ reduces the expression of cell cycle-related proteins, such as cyclin D1, cyclin A, and cyclin B1, but causes an increase in the p21Waf1/Cip1 level. The molecular mechanism by which CPZ regulates p21Waf1/Cip1 expression is unknown. Here, we provide evidence that CPZ activates the p21Waf1/Cip1 gene promoter via induction of the transcription factor early growth response-1 (Egr-1) independently of p53 in C6 cells. A point mutation in the Egr-1-binding motif within the p21Waf1/Cip1 promoter abrogated promoter inducibility due to CPZ. Forced expression of Egr-1 enhanced p21Waf1/Cip1 promoter activity. In contrast, knockdown of endogenous Egr-1 by small interference RNA attenuated CPZ-induced p21Waf1/Cip1 promoter activity. A chromatin immunoprecipitation assay demonstrated that Egr-1 binds to the p21Waf1/Cip1 gene promoter. Further analysis showed that the ERK and JNK MAP kinases are required for induction of Egr-1 by CPZ. Finally, stable silencing of Egr-1 expression lead to attenuated CPZ-inducible p21Waf1/Cip1 expression and inhibited G2/M phase cell-cycle arrest. These results demonstrate that a functional link between ERK and JNK MAP kinase pathways and p21Waf1/Cip1 induction via Egr-1 contributes to CPZ-induced anticancer activity in C6 glioma cells." @default.
• W1963537728 created "2016-06-24" @default.
• W1963537728 creator A5002034433 @default.
• W1963537728 creator A5013171066 @default.
• W1963537728 creator A5022736152 @default.
• W1963537728 creator A5045747630 @default.
• W1963537728 creator A5057530985 @default.
• W1963537728 creator A5063587278 @default.
• W1963537728 date "2010-01-01" @default.
• W1963537728 modified "2023-10-11" @default.
• W1963537728 title "Chlorpromazine activates p21^Waf1/Cip1gene transcription via early growth response-1 (Egr-1) in C6 glioma cells" @default.
• W1963537728 cites W1921003572 @default.
• W1963537728 cites W1967714756 @default.
• W1963537728 cites W1978283073 @default.
• W1963537728 cites W1978618811 @default.
• W1963537728 cites W1979742413 @default.
• W1963537728 cites W1987957628 @default.
• W1963537728 cites W1990340849 @default.
• W1963537728 cites W1996068610 @default.
• W1963537728 cites W1997135088 @default.
• W1963537728 cites W2003448771 @default.
• W1963537728 cites W2008117987 @default.
• W1963537728 cites W2015519364 @default.
• W1963537728 cites W2017907368 @default.
• W1963537728 cites W2027070465 @default.
• W1963537728 cites W2033692899 @default.
• W1963537728 cites W2033967764 @default.
• W1963537728 cites W2035568149 @default.
• W1963537728 cites W2048759700 @default.
• W1963537728 cites W2056778760 @default.
• W1963537728 cites W2061583132 @default.
• W1963537728 cites W2062968089 @default.
• W1963537728 cites W2068661207 @default.
• W1963537728 cites W2072088194 @default.
• W1963537728 cites W2073941950 @default.
• W1963537728 cites W2075514051 @default.
• W1963537728 cites W2083129294 @default.
• W1963537728 cites W2087610816 @default.
• W1963537728 cites W2089218510 @default.
• W1963537728 cites W2090343947 @default.
• W1963537728 cites W2117761646 @default.
• W1963537728 cites W2118457425 @default.
• W1963537728 cites W2138414621 @default.
• W1963537728 cites W2152118923 @default.
• W1963537728 cites W2152158740 @default.
• W1963537728 cites W2152913480 @default.
• W1963537728 cites W2315691109 @default.
• W1963537728 cites W4251000934 @default.
• W1963537728 doi "https://doi.org/10.3858/emm.2010.42.5.041" @default.
• W1963537728 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2877254" @default.
• W1963537728 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20368687" @default.
• W1963537728 hasPublicationYear "2010" @default.
• W1963537728 type Work @default.
• W1963537728 sameAs 1963537728 @default.
• W1963537728 citedByCount "51" @default.
• W1963537728 countsByYear W19635377282012 @default.
• W1963537728 countsByYear W19635377282013 @default.
• W1963537728 countsByYear W19635377282014 @default.
• W1963537728 countsByYear W19635377282015 @default.
• W1963537728 countsByYear W19635377282016 @default.
• W1963537728 countsByYear W19635377282017 @default.
• W1963537728 countsByYear W19635377282018 @default.
• W1963537728 countsByYear W19635377282019 @default.
• W1963537728 countsByYear W19635377282020 @default.
• W1963537728 countsByYear W19635377282021 @default.
• W1963537728 countsByYear W19635377282022 @default.
• W1963537728 countsByYear W19635377282023 @default.
• W1963537728 crossrefType "journal-article" @default.
• W1963537728 hasAuthorship W1963537728A5002034433 @default.
• W1963537728 hasAuthorship W1963537728A5013171066 @default.
• W1963537728 hasAuthorship W1963537728A5022736152 @default.
• W1963537728 hasAuthorship W1963537728A5045747630 @default.
• W1963537728 hasAuthorship W1963537728A5057530985 @default.
• W1963537728 hasAuthorship W1963537728A5063587278 @default.
• W1963537728 hasBestOaLocation W19635377281 @default.
• W1963537728 hasConcept C101762097 @default.
• W1963537728 hasConcept C104317684 @default.
• W1963537728 hasConcept C119056186 @default.
• W1963537728 hasConcept C134320426 @default.
• W1963537728 hasConcept C150194340 @default.
• W1963537728 hasConcept C153911025 @default.
• W1963537728 hasConcept C16438837 @default.
• W1963537728 hasConcept C166703698 @default.
• W1963537728 hasConcept C173396325 @default.
• W1963537728 hasConcept C184235292 @default.
• W1963537728 hasConcept C199835354 @default.
• W1963537728 hasConcept C29537977 @default.
• W1963537728 hasConcept C502942594 @default.
• W1963537728 hasConcept C55493867 @default.
• W1963537728 hasConcept C57074206 @default.
• W1963537728 hasConcept C67705224 @default.
• W1963537728 hasConcept C86339819 @default.
• W1963537728 hasConcept C86803240 @default.
• W1963537728 hasConcept C95444343 @default.
• W1963537728 hasConceptScore W1963537728C101762097 @default.
• W1963537728 hasConceptScore W1963537728C104317684 @default.
• W1963537728 hasConceptScore W1963537728C119056186 @default.
• W1963537728 hasConceptScore W1963537728C134320426 @default.

• next