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• W1963545723 abstract "We present a pulse sequence for the simultaneous measurement of N-H and Calpha-Halpha couplings in double-labeled proteins from 2D spectra. The proposed sequence, a modification of the HN(CO)CA experiment, combines the J-modulation method and the IPAP scheme. The couplings can be readily retrieved from a series of 2D (15)N-(1)H correlation spectra, differing in the time point at which a (1)H 180 degrees pulse is applied. This induces an intensity modulation of the (15)N-(1)H correlation peaks with the Calpha-Halpha coupling. The Calpha-Halpha coupling is then obtained by fitting the observed intensities to the modulation equation. The N-H coupling is measured in each member of the set from peak-to-peak separations in the IPAP subspectra. The pulse sequence is experimentally verified with a sample of (15)N/(13)C-enriched ubiquitin." @default.
• W1963545723 created "2016-06-24" @default.
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• W1963545723 date "2009-01-01" @default.
• W1963545723 modified "2023-10-17" @default.
• W1963545723 title "Simultaneous measurement of NH and CαHα coupling constants in proteins" @default.
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• W1963545723 doi "https://doi.org/10.1002/mrc.2532" @default.
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