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- W1963551132 abstract "A combination of high-performance-liquid-chromatography (HPLC), sensitive radioimmunoassays and a homologous in vitro bioassay was used to characterise human parathyroid hormone (hPTH)-peptides in human parathyroid adenoma and plasma. Chromatography of several synthetic hPTH-peptides allows the calibration of the HPLC column. On the basis of sequence hydrophobicity the elution position of peptides can be predicted. A model for the determination of the minimal peptide sequence of each peptide has been developed which based on immunological and physico-chemical properties allows the characterisation of unknown hPTH-peptides. Using this technique the heterogeneity of circulating hPTH-peptides in human plasma has been examined. Plasma extracts from healthy individuals, osteoporotic, hyperparathyroid and pseudo-hyperparathyroid patients were investigated. A uniform pattern in the heterogeneity of hPTH-peptides was detected. Using parathyroid adenoma as reference disease specific changes were characterised." @default.
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- W1963551132 date "1984-09-01" @default.
- W1963551132 modified "2023-10-14" @default.
- W1963551132 title "Analysis of immunoreactive and biologically active human parathyroid hormone-peptides by high-performance-liquid-chromatography" @default.
- W1963551132 doi "https://doi.org/10.1530/acta.0.1070060" @default.
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