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- W1963562106 abstract "Rat pituitary tumour cells in monolayer culture (GH3 cells) respond to oestradiol-17β and testosterone with increased synthesis of prolactin. The present study was undertaken to establish whether this effect of testosterone could be mediated via conversion to oestradiol-17β. Cell cultures were incubated with 400 μCi of [7α-3H]-testosterone at a concentration of 10−6 M for 96 h. The metabolites were isolated and identified by repeated paper chromatography, derivative formation and final crystallization to constant specific activity from different solvents.Testosterone was metabolized to a great extent, 88–96%, but no conversion (<0.0001%) of testosterone to oestradiol-17β or oestrone could be detected. 5α-Dihydrotestosterone and 5α-androstane-3β,17β-diol were found as the major metabolites, isolated in a radiochemically pure form. 5α-Androstane-3α,17β-diol and 4-androstene-3,17-dione were also isolated and identified, and they constituted a minor fraction, 5 and 0.2%, respectively.The kinetics of the metabolism of [3H]- testosterone were studied by harvesting the cell cultures after 6, 12, 24, 48, and 96 h. The data suggested that 5α-androstane-3β,17β-diol was formed via 5α-dihydro-testosterone, that was subsequently shown to be a good substrate for the formation of the former.It is concluded that the stimulatory effect of testosterone on prolactin secretion by these cells is probably not mediated via conversion to oestradiol-17β. The extensive metabolism of testosterone opens the possibility that the effect of testosterone is in some way related to the formation of a metabolite." @default.
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- W1963562106 date "1979-10-01" @default.
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- W1963562106 title "Metabolism of [3H]-testosterone and [1,2-3H]-dihydrotestosterone by prolactin secreting rat pituitary tumour cells in culture" @default.
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- W1963562106 doi "https://doi.org/10.1016/0022-4731(79)90104-3" @default.
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