Matches in SemOpenAlex for { <https://semopenalex.org/work/W1963563485> ?p ?o ?g. }
- W1963563485 endingPage "935" @default.
- W1963563485 startingPage "929" @default.
- W1963563485 abstract "RNA interference (RNAi) has been successfully employed for specific inhibition of gene expression; however, safety and delivery of RNAi remain critical issues. We investigated the combinatorial use of RNAi and U1 interference (U1i). U1i is a gene-silencing technique that acts on the pre-mRNA by preventing polyadenylation. RNAi and U1i have distinct mechanisms of action in different cellular compartments and their combined effect allows usage of minimal doses, thereby avoiding toxicity while retaining high target inhibition. As a proof of concept, we investigated knockdown of the firefly luciferase reporter gene by combinatorial use of RNAi and U1i, and evaluated their inhibitory potential both in vitro and in vivo. Co-transfection of RNAi and U1i constructs showed additive reduction of luciferase expression up to 95% in vitro. We attained similar knockdown when RNAi and U1i constructs were hydrodynamically transfected into murine liver, demonstrating for the first time successful in vivo application of U1i. Moreover, we demonstrated long-term gene silencing by AAV-mediated transduction of murine muscle with RNAi/U1i constructs targeting firefly luciferase. In conclusion, these results provide a proof of principle for the combinatorial use of RNAi and U1i to enhance target gene knockdown in vivo." @default.
- W1963563485 created "2016-06-24" @default.
- W1963563485 creator A5006717752 @default.
- W1963563485 creator A5011351945 @default.
- W1963563485 creator A5029353825 @default.
- W1963563485 creator A5032183121 @default.
- W1963563485 creator A5038524181 @default.
- W1963563485 creator A5040613970 @default.
- W1963563485 creator A5055789630 @default.
- W1963563485 creator A5067045436 @default.
- W1963563485 creator A5072847313 @default.
- W1963563485 creator A5089477827 @default.
- W1963563485 date "2011-04-07" @default.
- W1963563485 modified "2023-10-17" @default.
- W1963563485 title "AAV-mediated in vivo knockdown of luciferase using combinatorial RNAi and U1i" @default.
- W1963563485 cites W1499318130 @default.
- W1963563485 cites W1573066622 @default.
- W1963563485 cites W1647075334 @default.
- W1963563485 cites W1771985274 @default.
- W1963563485 cites W1967944402 @default.
- W1963563485 cites W1967959947 @default.
- W1963563485 cites W1971643509 @default.
- W1963563485 cites W1982690424 @default.
- W1963563485 cites W1989454102 @default.
- W1963563485 cites W1992517046 @default.
- W1963563485 cites W1994262262 @default.
- W1963563485 cites W2000729283 @default.
- W1963563485 cites W2006213560 @default.
- W1963563485 cites W2014821738 @default.
- W1963563485 cites W2016412275 @default.
- W1963563485 cites W2029971664 @default.
- W1963563485 cites W2030621748 @default.
- W1963563485 cites W2037495678 @default.
- W1963563485 cites W2037639054 @default.
- W1963563485 cites W2047178946 @default.
- W1963563485 cites W2051887414 @default.
- W1963563485 cites W2078035839 @default.
- W1963563485 cites W2099517096 @default.
- W1963563485 cites W2101098226 @default.
- W1963563485 cites W2101627876 @default.
- W1963563485 cites W2111941264 @default.
- W1963563485 cites W2114741749 @default.
- W1963563485 cites W2116660518 @default.
- W1963563485 cites W2122162055 @default.
- W1963563485 cites W2131947999 @default.
- W1963563485 cites W2144325780 @default.
- W1963563485 cites W2151488213 @default.
- W1963563485 cites W2153074755 @default.
- W1963563485 cites W2157279213 @default.
- W1963563485 cites W2160005534 @default.
- W1963563485 cites W2166903671 @default.
- W1963563485 doi "https://doi.org/10.1038/gt.2011.41" @default.
- W1963563485 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3169806" @default.
- W1963563485 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21472008" @default.
- W1963563485 hasPublicationYear "2011" @default.
- W1963563485 type Work @default.
- W1963563485 sameAs 1963563485 @default.
- W1963563485 citedByCount "14" @default.
- W1963563485 countsByYear W19635634852012 @default.
- W1963563485 countsByYear W19635634852013 @default.
- W1963563485 countsByYear W19635634852014 @default.
- W1963563485 countsByYear W19635634852015 @default.
- W1963563485 countsByYear W19635634852017 @default.
- W1963563485 countsByYear W19635634852019 @default.
- W1963563485 countsByYear W19635634852020 @default.
- W1963563485 countsByYear W19635634852022 @default.
- W1963563485 crossrefType "journal-article" @default.
- W1963563485 hasAuthorship W1963563485A5006717752 @default.
- W1963563485 hasAuthorship W1963563485A5011351945 @default.
- W1963563485 hasAuthorship W1963563485A5029353825 @default.
- W1963563485 hasAuthorship W1963563485A5032183121 @default.
- W1963563485 hasAuthorship W1963563485A5038524181 @default.
- W1963563485 hasAuthorship W1963563485A5040613970 @default.
- W1963563485 hasAuthorship W1963563485A5055789630 @default.
- W1963563485 hasAuthorship W1963563485A5067045436 @default.
- W1963563485 hasAuthorship W1963563485A5072847313 @default.
- W1963563485 hasAuthorship W1963563485A5089477827 @default.
- W1963563485 hasBestOaLocation W19635634851 @default.
- W1963563485 hasConcept C104317684 @default.
- W1963563485 hasConcept C111335760 @default.
- W1963563485 hasConcept C119056186 @default.
- W1963563485 hasConcept C153911025 @default.
- W1963563485 hasConcept C166703698 @default.
- W1963563485 hasConcept C173396325 @default.
- W1963563485 hasConcept C190232843 @default.
- W1963563485 hasConcept C207001950 @default.
- W1963563485 hasConcept C22615655 @default.
- W1963563485 hasConcept C54009773 @default.
- W1963563485 hasConcept C54355233 @default.
- W1963563485 hasConcept C67705224 @default.
- W1963563485 hasConcept C86803240 @default.
- W1963563485 hasConcept C95444343 @default.
- W1963563485 hasConceptScore W1963563485C104317684 @default.
- W1963563485 hasConceptScore W1963563485C111335760 @default.
- W1963563485 hasConceptScore W1963563485C119056186 @default.
- W1963563485 hasConceptScore W1963563485C153911025 @default.
- W1963563485 hasConceptScore W1963563485C166703698 @default.
- W1963563485 hasConceptScore W1963563485C173396325 @default.