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• W1963573234 abstract "Subsurface injection of oxygen-releasing materials (ORMs) is frequently performed at petroleum-contaminated sites to stimulate aerobic bioremediation of benzene, toluene, ethylbenzene, and xylenes (BTEX). In this study, qPCR enumeration of aromatic oxygenase genes and PCR-DGGE profiles of bacterial 16S rRNA genes were combined with groundwater monitoring to determine the impact of ORM injection on BTEX bioremediation at a gasoline-contaminated site. Prior to injection, BTEX concentrations were greater than 3 mg/L and DO levels were typically lessthan 2 mg/L, butphenol hydroxylase (PHE) and ring-hydroxylating toluene monooxygenase (RMO) genes were detected in impacted wells indicating the potential for aerobic BTEX biodegradation. Following injection, DO increased, BTEX concentrations decreased substantially, and PHE and RMO genes copies increased by 1-3 orders of magnitude. In addition, naphthalene dioxygenase (NAH) and xylene monooxygenase (TOL) genes were intermittently detected during periods of increased DO. Following depletion of the ORM, DO decreased, BTEX concentrations rebounded, and oxygenase genes were no longer detected. Temporal changes in PCR-DGGE microbial community profiles reflected the dynamic changes in subsurface conditions. Overall, the combination of chemical and geochemical analyses with quantification of aromatic oxygenase genes demonstrated that injection stimulated BTEX biodegradation until the ORM was depleted." @default.
• W1963573234 created "2016-06-24" @default.
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• W1963573234 date "2009-02-13" @default.
• W1963573234 modified "2023-10-16" @default.
• W1963573234 title "Quantification of Aromatic Oxygenase Genes to Evaluate Enhanced Bioremediation by Oxygen Releasing Materials at a Gasoline-Contaminated Site" @default.
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• W1963573234 doi "https://doi.org/10.1021/es900146f" @default.
• W1963573234 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19368209" @default.
• W1963573234 hasPublicationYear "2009" @default.
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