FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1963578252> ?p ?o ?g. }

• W1963578252 endingPage "253" @default.
• W1963578252 startingPage "241" @default.
• W1963578252 abstract "Neutrophil (PMN) contributions to the acute inflammatory process and host defense include generation of bioreactive oxygen metabolites and secretion of granule enzymes. We assessed equine PMN secretion using several PMN stimuli, singly and in combination with bacterial lipopolysaccharide (LPS). LPS avidly associated with equine PMN, as shown by strong PMN labeling with FITC-conjugated LPS. LPS alone (1 or 10 micrograms ml-1) was a weak stimulus for PMN superoxide anion (O2-) generation, but preincubation with LPS followed by phorbol ester (PMA, 10 ng ml-1) significantly augmented (P less than 0.01) secretion of O2- (19.38 nmol O2- per 2 x 10(6) PMN per 5 min) over the amount generated by PMA stimulation alone (13.75 nmol O2-). A qualitatively similar, but smaller O2(-)-generation response occurred when either opsonized zymosan or recombinant human C5a was used as the PMN stimulus. Arachidonic acid (ArA; 50-200 microM) was a potent stimulus, with secreted O2- levels similar to those from PMA-stimulated PMN. Preincubation of PMN with either the formyl peptide, fMLP, or platelet-activating factor before stimulation with ArA did not significantly increase O2- generation over levels obtained using ArA alone. Release of PMN granule enzymes was also quantitated. A small amount of lysozyme secretion resulted when PMN were exposed to LPS alone (8.20% of total cell content), and PMA stimulation caused marked release of PMN lysozyme (44.45%). Non-specific proteolytic activity in PMN supernatants, assessed by cleavage of a collagen-rich substrate, was minimal with LPS as a sole stimulus (5.08%). There was significant proteolytic activity (P less than 0.01) in supernatants from PMA-stimulated PMN (27.21%), and preincubation with LPS followed by PMA stimulation slightly enhanced (P less than 0.05) the release of PMN proteases (34.62%). The activities of beta-glucuronidase, acid phosphatase, and alkaline phosphatase were minimal in PMN supernatants when using LPS and PMA as stimuli. The activity of PMN granule enzymes was found to be sensitive to the presence of normal equine serum, and proteolytic activity was markedly reduced (80.13% reduction) in the presence of 10% pooled serum." @default.
• W1963578252 created "2016-06-24" @default.
• W1963578252 creator A5039976043 @default.
• W1963578252 creator A5048763292 @default.
• W1963578252 creator A5086509371 @default.
• W1963578252 date "1992-03-01" @default.
• W1963578252 modified "2023-09-27" @default.
• W1963578252 title "Secretory activity of equine polymorphonuclear leukocytes: stimulus specificity and priming effects of bacterial lipopolysaccharide" @default.
• W1963578252 cites W1480655900 @default.
• W1963578252 cites W1598208909 @default.
• W1963578252 cites W1925642872 @default.
• W1963578252 cites W1967110335 @default.
• W1963578252 cites W1981442508 @default.
• W1963578252 cites W1984678934 @default.
• W1963578252 cites W2006767145 @default.
• W1963578252 cites W2026721162 @default.
• W1963578252 cites W2038583951 @default.
• W1963578252 cites W2038748367 @default.
• W1963578252 cites W2092528394 @default.
• W1963578252 cites W2120227455 @default.
• W1963578252 cites W2145090129 @default.
• W1963578252 cites W2168887400 @default.
• W1963578252 cites W2181624522 @default.
• W1963578252 cites W2260018086 @default.
• W1963578252 cites W2337579403 @default.
• W1963578252 cites W2395452535 @default.
• W1963578252 cites W2397172104 @default.
• W1963578252 cites W4235505198 @default.
• W1963578252 cites W4248387811 @default.
• W1963578252 doi "https://doi.org/10.1016/0165-2427(92)90012-f" @default.
• W1963578252 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1317072" @default.
• W1963578252 hasPublicationYear "1992" @default.
• W1963578252 type Work @default.
• W1963578252 sameAs 1963578252 @default.
• W1963578252 citedByCount "16" @default.
• W1963578252 countsByYear W19635782522012 @default.
• W1963578252 crossrefType "journal-article" @default.
• W1963578252 hasAuthorship W1963578252A5039976043 @default.
• W1963578252 hasAuthorship W1963578252A5048763292 @default.
• W1963578252 hasAuthorship W1963578252A5086509371 @default.
• W1963578252 hasConcept C126322002 @default.
• W1963578252 hasConcept C134018914 @default.
• W1963578252 hasConcept C151730666 @default.
• W1963578252 hasConcept C181199279 @default.
• W1963578252 hasConcept C198677920 @default.
• W1963578252 hasConcept C202751555 @default.
• W1963578252 hasConcept C203014093 @default.
• W1963578252 hasConcept C24998067 @default.
• W1963578252 hasConcept C2776966407 @default.
• W1963578252 hasConcept C2777983669 @default.
• W1963578252 hasConcept C2778754761 @default.
• W1963578252 hasConcept C2778870864 @default.
• W1963578252 hasConcept C2780795997 @default.
• W1963578252 hasConcept C49039625 @default.
• W1963578252 hasConcept C49760210 @default.
• W1963578252 hasConcept C55493867 @default.
• W1963578252 hasConcept C71924100 @default.
• W1963578252 hasConcept C86803240 @default.
• W1963578252 hasConcept C99971728 @default.
• W1963578252 hasConceptScore W1963578252C126322002 @default.
• W1963578252 hasConceptScore W1963578252C134018914 @default.
• W1963578252 hasConceptScore W1963578252C151730666 @default.
• W1963578252 hasConceptScore W1963578252C181199279 @default.
• W1963578252 hasConceptScore W1963578252C198677920 @default.
• W1963578252 hasConceptScore W1963578252C202751555 @default.
• W1963578252 hasConceptScore W1963578252C203014093 @default.
• W1963578252 hasConceptScore W1963578252C24998067 @default.
• W1963578252 hasConceptScore W1963578252C2776966407 @default.
• W1963578252 hasConceptScore W1963578252C2777983669 @default.
• W1963578252 hasConceptScore W1963578252C2778754761 @default.
• W1963578252 hasConceptScore W1963578252C2778870864 @default.
• W1963578252 hasConceptScore W1963578252C2780795997 @default.
• W1963578252 hasConceptScore W1963578252C49039625 @default.
• W1963578252 hasConceptScore W1963578252C49760210 @default.
• W1963578252 hasConceptScore W1963578252C55493867 @default.
• W1963578252 hasConceptScore W1963578252C71924100 @default.
• W1963578252 hasConceptScore W1963578252C86803240 @default.
• W1963578252 hasConceptScore W1963578252C99971728 @default.
• W1963578252 hasIssue "3-4" @default.
• W1963578252 hasLocation W19635782521 @default.
• W1963578252 hasLocation W19635782522 @default.
• W1963578252 hasOpenAccess W1963578252 @default.
• W1963578252 hasPrimaryLocation W19635782521 @default.
• W1963578252 hasRelatedWork W1963578252 @default.
• W1963578252 hasRelatedWork W1983297645 @default.
• W1963578252 hasRelatedWork W1987367775 @default.
• W1963578252 hasRelatedWork W2040323505 @default.
• W1963578252 hasRelatedWork W2400393202 @default.
• W1963578252 hasRelatedWork W2406744790 @default.
• W1963578252 hasRelatedWork W2410419920 @default.
• W1963578252 hasRelatedWork W2429147379 @default.
• W1963578252 hasRelatedWork W2726028516 @default.
• W1963578252 hasRelatedWork W4252625852 @default.
• W1963578252 hasVolume "31" @default.
• W1963578252 isParatext "false" @default.
• W1963578252 isRetracted "false" @default.
• W1963578252 magId "1963578252" @default.
• W1963578252 workType "article" @default.