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- W1963625848 abstract "The quantitative analysis of 2-keto-L-gulonic acid (2-KLG) produced by microbial fermentation is described. 2-KLG is separated from other aldonic and ketoaldonic acids by high-performance liquid chromatography on an Aminex anion exchange column with ammonium formate or potassium phosphate as the eluant. This is a rapid and simple method for routine analysis of a large number of samples generated by fermentation studies. Gas chromatography--mass spectrometry permits the qualitative and quantitative analysis of nanogram levels of 2-keto-L-gulonate in complex media and provides confirmation of the HPLC results. The methodologies presented are useful for the analysis of a number of aldonic and ketoaldonic acids." @default.
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- W1963625848 date "1986-09-01" @default.
- W1963625848 modified "2023-09-25" @default.
- W1963625848 title "Determination of 2-keto-l-gulonic and other ketoaldonic and aldonic acids produced by ketogenic bacterial fermentation" @default.
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- W1963625848 doi "https://doi.org/10.1016/0003-2697(86)90638-x" @default.
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